A5041043882- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Food Safety and Hygiene
- Antibiotic Resistance in Bacteria
- Microbial Inactivation Methods
- Identification and Quantification in Food
- Bacteriophages and microbial interactions
- Meat and Animal Product Quality
- Animal Nutrition and Physiology
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Enterobacteriaceae and Cronobacter Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Plasma Applications and Diagnostics
- Bacterial Identification and Susceptibility Testing
- Probiotics and Fermented Foods
- Essential Oils and Antimicrobial Activity
- Helicobacter pylori-related gastroenterology studies
- Molecular Biology Techniques and Applications
- Vibrio bacteria research studies
- Antibiotics Pharmacokinetics and Efficacy
- Infections and bacterial resistance
- Aquaculture disease management and microbiota
- Spectroscopy and Chemometric Analyses
University of Bologna
2015-2024
Agriculture and Food
2018
Defense Information Systems Agency
2012
A prototype decision support IT-tool for the food industry was developed in STARTEC project. Typical processes and steps were mapped using real life production scenarios of participating companies manufacturing complex ready-to-eat foods. Companies looked a more integrated approach when making safety decisions that would align with existing HACCP systems. The tool designed shelf assessments data on safety, quality, costs, pasta salad meal as case product. process flow chart used starting...
Listeria monocytogenes is a foodborne pathogen adapted to survive and persist in multiple environments. Following two previous studies on prevalence virulence of L. ST121 ST14 repeatedly collected the same rabbit-meat processing plant, research questions present study were to: 1) assess persistence isolates from rabbit-plant; 2) select genes associated physiological adaptation food-processing environment; 3) compare presence/absence/truncation these newly sequenced publicly available...
The massive and irrational use of antibiotics in livestock productions has fostered the occurrence spread resistance to "old class antimicrobials." To cope with that phenomenon, some regulations have been already enforced member states European Union. However, a role animals relatively recent alerts on rapid worldwide increase last-choice antimicrobials as carbapenems is very unlikely. Conversely, these are increasingly administered veterinary hospitals whose spreading bacteria or mobile...
Sewage metagenomics has risen to prominence in urban population surveillance of pathogens and antimicrobial resistance (AMR). Unknown species with similarity known genomes cause database bias reference-based metagenomics. To improve surveillance, we seek recover sewage develop a quantification correlation workflow for these AMR over time. We use longitudinal sampling seven treatment plants from five major European cities explore the utility catch-all sequencing population-level samples....
The COVID-19 pandemic has significantly impacted human society at many levels, from public health to economics and transports, highlighting the need of approaches integrating all available information better understand model similar phenomena, also in order develop early detection responses. In this paper we show result an analysis metropolitan area Bologna, Italy, epidemiological mathematical model, SARS-CoV-2 virus quantification wastewater, clinical hospitalization, vaccination campaign,...
Salmonella enterica ser. Typhimurium monophasic variant 4,[5],12:i:- has been associated with food-borne epidemics worldwide and swine appeared to be the main reservoir in most of countries isolation. However, monomorphic nature this serovar has, so far, hindered identification source due expansion clonal lineages multiple hosts food producing systems. Since geographically structured genetic signals can shape bacterial populations, biogeographical markers S. 1,4,[5],12:i:- genomes contribute...
Due to the increasing number of studies reporting detection antimicrobial-resistant isolates
Abstract Increasing reports on K. pneumoniae strains with antimicrobial resistance and virulence traits from food farm animals are raising concerns about the potential role of Klebsiella spp. as a foodborne pathogen. This study aimed to report characterize isolates two artisanal ready-to-eat (soft cheese salami) producing facilities, track similar genotypes in different ecological niches. Over 1170 samples were collected during whole production chain batches. The overall prevalence was 6%....
The complete nucleotide sequence of the 12 656 bp plasmid pFPTB1 from Salmonella enterica subsp. serovar Typhimurium, which mediates resistance to tetracyclines and ampicillin, was determined. analysed for potential reading frames structural features indicative transposons transposon relics.Plasmid transformed into Escherichia coli JM109, overlapping restriction fragments were cloned E. vectors sequenced. In vitro susceptibility testing carried out confirm phenotype mediated by this...
Klebsiella pneumoniae is the most common species infecting animals and one of causing agents mastitis in cows. The rise antimicrobial resistance K. pneumoniae, particularly strains producing extended-spectrum β-lactamases (ESBLs) and/or carbapenemases, concern worldwide. Recently (Regulation UE No 2022/1255), carbapenems cephalosporins combination with β-lactamase inhibitors have been reserved only to human treatments European Union. aim this study was investigate role cattle as carrier...
Sewage metagenomics has risen to prominence in urban population surveillance of pathogens and antimicrobial resistance (AMR). Unknown species with similarity known genomes cause database bias reference-based metagenomics. To improve surveillance, we designed this study recover sewage develop a quantification correlation workflow for these AMR over time. We used longitudinal sampling seven treatment plants from five major European cities explore the utility catch-all sequencing...
The growth and survival of Arcobacter butzleri cryaerophilus in milk were investigated at different storage temperatures. Three strains each species inoculated into ultrahigh-temperature (UHT), pasteurized, raw cow's stored 4, 10, 20°C for 6 days. spp. during was evaluated by a culture method. Results clearly showed that A. remained viable when 4°C 10°C period When UHT pasteurized 20°C, the count increased, with longer lag-phase milk, whereas increased first 48 h then rapidly decreased to...
Several strategies have been in place food animal production to reduce the unnecessary use of antimicrobial agents. Beyond monitoring their use, evaluation effect these on occurrence and types resistance (AMR) associated genes is crucial untangle potential emergence spread AMR humans through chain. In present study, was evaluated commensal Escherichia coli isolated from broiler carcasses "produced without antibiotics" 3 antibiotic-free (AB-free) farms Italy 2019. Sequenced data were analyzed...
Artisanal cheeses are produced in small-scale production plants, where the lack of full automation and control environmental processing parameters suggests a potential risk microbial contamination. The aim this study was to perform longitudinal survey an Italian artisanal factory producing spreadable soft cheese with no rind evaluate inter- intra-batch variability physicochemical on total 720 samples. Specifically samples, evaluation additionally performed parameters. Cheese samples were...
Listeria monocytogenes is a food-borne pathogen able to survive and grow in different environments including food processing plants where it can persist for month or years. In the present study discriminatory power of Whole Genome Sequencing (WGS)-based analysis (cgMLST) was compared that molecular typing methods on 34 L. isolates collected over one year same rabbit meat plant belonging three genotypes (ST14, ST121, ST224). Each genotype included indistinguishable by standard methods. The...