A5007222205- Salmonella and Campylobacter epidemiology
- Biosensors and Analytical Detection
- Identification and Quantification in Food
- Advanced biosensing and bioanalysis techniques
- Listeria monocytogenes in Food Safety
- Yersinia bacterium, plague, ectoparasites research
- Botulinum Toxin and Related Neurological Disorders
- Advanced Chemical Sensor Technologies
- Food Safety and Hygiene
- Diphtheria, Corynebacterium, and Tetanus
- Viral gastroenteritis research and epidemiology
- Advanced Biosensing Techniques and Applications
- Vibrio bacteria research studies
- Streptococcal Infections and Treatments
- Bacillus and Francisella bacterial research
- Molecular Biology Techniques and Applications
- Molecular Communication and Nanonetworks
- Probiotics and Fermented Foods
- Bacterial Identification and Susceptibility Testing
- Microbial Inactivation Methods
- Monoclonal and Polyclonal Antibodies Research
- Meat and Animal Product Quality
- Escherichia coli research studies
- Zoonotic diseases and public health
- Hepatitis Viruses Studies and Epidemiology
Istituto Superiore di Sanità
2015-2024
University of Rome Tor Vergata
2004
Abstract The RNA programmed non-specific (trans) nuclease activity of CRISPR-Cas Type V and VI systems has opened a new era in the field nucleic acid-based detection. Here, we report on enhancement trans-cleavage Cas12a enzymes using hairpin DNA sequences as FRET-based reporters. We discover faster rate due to its improved affinity (Km) for structures, provide mechanistic insights our findings through Molecular Dynamics simulations. Using probes significantly enhance signal transduction...
ABSTRACT The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, the extraction purification DNA from preenrichment culture, four methods (boiling, alkaline lysis, Nucleospin, Dynabeads Direct System I) were compared. most effective then combined with real-time PCR based on double-stranded binding dye SYBR Green I used ABI Prism 7700 system. specificity reaction determined by melting...
ABSTRACT Botulinum neurotoxin (BoNT), the most toxic substance known, is produced by spore-forming bacterium Clostridium botulinum and, in rare cases, also some strains of butyricum and baratii . The standard procedure for definitive detection BoNT-producing clostridia a culture method combined with using mouse bioassay (SMB). SMB highly sensitive specific, but it expensive time-consuming there are ethical concerns due to use laboratory animals. PCR provides rapid alternative initial...
An electrochemical enzyme-linked immunosorbent assay (ELISA) coupled with flow injection analysis (ELISA-FIA) and a PCR-based method using ST11 ST15 primers for detecting salmonellae in meat were evaluated comparison the International Organization Standardization (ISO) culture method. The methods applied to experimentally contaminated naturally samples. results showed that both ELISA-FIA PCR allowed detection of salmonella product low number microorganisms (1 10 salmonellae/25 g) after only...
Botulinum toxins (BoNTs) are classically produced by Clostridium botulinum but rarely also from neurotoxigenic strains of baratii and butyricum. BoNT type A (BoNT/A), BoNT/B, BoNT/E, very BoNT/F mainly responsible for human botulism. Standard microbiological methods take into consideration only the detection C. botulinum. The presumptive identification toxigenic together with typing has to be performed mouse bioassay. development PCR-based BoNT-producing clostridia would an ideal alternative...
Colorectal cancer (CRC) is a leading cause of death worldwide, and its incidence correlated with infections, chronic inflammation, diet, genetic factors. An emerging aspect that microbial dysbiosis infections triggered by certain bacteria can be risk factors for tumor progression. Recent data suggest bacterial toxins implicated in DNA attack or proliferation, replication, insurgence progression CRC. In this study, we recruited more than 300 biopsy specimens from people undergoing...
Abstract We report here the rational design and optimization of an antibody‐responsive, DNA‐based device that enables communication between pairs otherwise non‐interacting proteins. The is designed to recognize bind a specific antibody and, in response, undergo conformational change leads release DNA strand, termed “translator,” regulates activity downstream target protein. As proof principle, we demonstrate antibody‐induced control proteins thrombin Taq polymerase. resulting strategy...
Salmonella is one of the main organisms causing outbreaks foodborne illness, and meat major vehicles salmonellosis throughout world. A novel analytical immunosensor array, based on a 96-well electrochemical plate coupled with immunomagnetic beads (ELIME array), proposed for detection in samples. After an optimization study, using enterica serotype Enteritidis as reference antigen, ability method to interact large number serovars commonly present food was evaluated. The assay then used...
Abstract A sandwich ELISA assay using monoclonal and polyclonal antibodies against salmonella has been proposed. The activity of the label enzyme (horseradish peroxidase) is measured electrochemically 3,3′,5,5′ tetramethylbenzidine as substrate. efficiency specificity method were verified during preliminary studies on various serotypes Salmonella other bacteria commonly present in food (E. coli, Klebsiella pneumoniae, Morganella morganii, Citrobacter freundii, Yersinia enterocolitica). was...
Listeria monocytogenes is frequently found as a contaminant in raw and ready-to-eat foods. The ability of L. to multiply at refrigeration temperatures grow wide range pH values particular concern for food safety. According the European Union regulation on microbiological criteria foodstuffs, must be absent some categories standard method detection foods (ISO 11290-1: 1996 (ISO, International Organization Standardization)) cost time consuming. Developments rapid, cost-effective automated...
Abstract Glucose‐6‐phosphate dehydrogenase (G6PD) deficiency is one of the most well‐known human genetic defects, identified in more than 400 million individuals world. To date, no commercial kits are available for mutation screening this disease. Seventy G6PD‐deficient Italian admitted to Laboratory Clinical Molecular Biology Hospital “Agostino Gemelli” Rome were screened frequent mutations, by means allele‐specific PCR, followed restriction fragment length electrophoresis. The present...