A5085897348- Probiotics and Fermented Foods
- Listeria monocytogenes in Food Safety
- Meat and Animal Product Quality
- Salmonella and Campylobacter epidemiology
- Microbial Inactivation Methods
- Protein Hydrolysis and Bioactive Peptides
- Food Safety and Hygiene
- Fermentation and Sensory Analysis
- Identification and Quantification in Food
- Radiation Effects and Dosimetry
- Antibiotic Resistance in Bacteria
- Aquaculture disease management and microbiota
- Food Supply Chain Traceability
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Botanical Research and Applications
- Animal Nutrition and Physiology
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Milk Quality and Mastitis in Dairy Cows
- Sustainable Urban and Rural Development
- Sensory Analysis and Statistical Methods
- Viral gastroenteritis research and epidemiology
- Antioxidant Activity and Oxidative Stress
Istituto Zooprofilattico Sperimentale Della Sicilia
2015-2025
Institute for Veterinary Medical Research
2015
ABSTRACT The main hypothesis of this work was that Sicilian forestry resources are suitable for the production equipment to be used in cheese making and indigenous milk lactic acid bacteria (LAB) able develop stable biofilms providing starter nonstarter cultures necessary curd fermentation ripening, respectively. Hence, present carried out with deproteinized whey evaluate LAB biofilm formation on different woods derived from tree species grown Sicily. Microbiological scanning electron...
Three Lactococcus lactis subsp. cremoris strains were used to develop ad hoc biofilms on the surfaces of virgin wooden vats for cheese production. Two (TZ) tested under controlled conditions (pilot plant), and two (TA) uncontrolled (industrial plant). In each plant, one vat (TA1 TZ1) was control, traditional production PDO Vastedda della Valle del Belìce (Vastedda) cheese, (TA2 TZ2) experimental performed after lactococcal biofilm activation daily addition a natural whey starter culture...
Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence antimicrobial resistance profile of isolates recovered from different aquatic sources. Besides, searched seaweeds corresponding seawater samples. Bacteriological molecular methods applied to 100 samples led isolation 28 27 The highest prevalence was detected rivers followed by artificial ponds, streams, well waters, spring waters....
Listeria monocytogenes is an important foodborne pathogen, markedly persistent even in harsh environments and responsible for high hospitalization mortality rates. The aim of the present study was to detect strains circulating Sicily over a five-year period characterize their antimicrobial resistance profiles. key element this sharing data among various entities involved food control clinical surveillance listeriosis order develop integrated approach pathogen. A total 128 isolates were...
A case of staphylococcal food poisoning was observed in two individuals the same family after consumption primosale, a semiripened sheep cheese produced Sicily. Staphylococcus aureus isolated from enterotoxin C (SEC) and carried both (sec) toxic shock syndrome toxin (tsst-1) gene. Following this case, an extensive survey conducted on 971 samples (raw milk, cheese, meat, preparations). S. detected 102 samples, all types with exception ricotta cheese. The tsst-1 gene present 42% strains,...
Fresh produce occupies an increasingly important place in the human food supply because of its health-promoting nutritional properties. Most fresh is eaten raw or after minimal processing and, consequently, pathogen contamination can represent a serious health risk. There has been increase foodborne outbreaks and cases associated with produce, but literature data about prevalence are inconsistent. This study was undertaken to assess hygienic quality most common bacterial pathogens sold...
<em>Listeria monocytogenes</em> is a pathogen frequently found in dairy products, and its growth difficult to control. Bacteriocinlike inhibitory substances (BLIS), produced by lactic acid bacteria (LAB), having proven <em>in vitro</em> anti-<em>Listeria</em> activity, could provide an innovative approach control <em>L. monocytogenes</em>; however, this application needs be evaluated vivo</em>. In study, twenty LAB strains isolated from...
spp. are among the most frequent causes of foodborne diseases, and increasing occurrence MDR strains is an additional cause for concern. In three-year period 2019-2021, we collected
Seafood can vehiculate foodborne illnesses from water to humans. Climate changes, increasing contamination and coastlines anthropization, favor the global spread of Vibrio spp. occurrence antibiotic-resistant isolates. The aim this study was evaluate potentially pathogenic in fishery products collected Sicily assess their antibiotic resistance. Bacteriological molecular methods were applied 603 seafood samples detect V. parahaemolyticus, cholerae, vulnificus, alginolyticus order...
Notification of foodborne outbreaks has been mandatory in Europe since 2005, and surveillance is carried out along the entire food chain. Here we report results obtained from laboratory investigations about four cases that occurred Sicily between 2009 2016, deemed to be related staphylococcal enterotoxins (SEs) coagulase-positive Staphylococci (CPS) by Local Public Health Authority. Primosale cheese samples were processed culture methods for enumeration CPS immunoenzymatic assays detection...
Extra-intestinal E. coli are emerging as a global threat due to their diffusion opportunistic pathogens and, above all, wide set of antibiotic resistance determinants. There still many gaps in our knowledge origin and spread pathways, although food animals have been adjudicated vehicles for passing mult-drug resistant bacteria humans. This study analyzed 46 samples meat purchased from retail stores Palermo order obtain quinolone-resistant isolates. Strains were screened phylogenetic groups,...
In Sicily, the current increasing cultivation of Opuntia ficus-indica corresponds to an availability prickly pear by-product (PPB) that results from fruit processing for juice extraction. This investigation aims evaluate nutritional traits PPB ruminant feeding and its stability during a 21-day outdoor storage, using potassium metabisulfite (PMB) as preservative agent, added mass at different doses (0, 50, 100, 150 g/kg). The fractioning showed it included 28% peel pulp 72% seeds on dry...
This work was carried out with the aim to reduce transformation duration of Protected Designation Origin (PDO) Pecorino Siciliano cheese. To this purpose, cooking in hot water (experimental production, EXP) compared traditional cheese under whey permeate (control CTR). The microbiological composition rind (UR) and core (Co) section CTR EXP cheeses determined by a combined culture-dependent -independent approach. Total mesophilic microorganisms lactic acid bacteria (LAB) present raw ewes'...
Ovine ricotta cheese is a traditional Sicilian dairy product characterised by high humidity and short shelf life (2-4 days when refrigerated). The increasing demand for fresh food has prompted manufacturers to develop special packaging techniques, such as modified atmosphere (MAP), that can extend the maintain organoleptic characteristics of ovine cheese. aim present study was evaluate MAP-packed monitoring microbiological, chemical, physical product. Samples single batch were packed in MAP...
Traditional Sicilian cheese productions are carried out employing traditional wooden vats, called tina. Many studies have highlighted the beneficial role of dairy equipment by contributing to enriching milk microflora and improving acidification processes. The present work was undertaken evaluate safety vats used coagulate milk. To this purpose, different microbial populations hosted onto internal surfaces produce two stretched cheeses, namely Caciocavallo Palermitano Vastedda della valle...
The aim of the study was to evaluate occurrence Arcobacter spp. in food samples collected from Sicilia region. A total 91 products animal origin (41 meat, 17 fresh milk, 18 shellfish) and 15 vegetables, were examined by cultural method confirmed biochemical analysis PCR methods. detection performed, after selective enrichment, on two agar plates: mCCD (modified charcoal cefoperazone deoxycholate) supplemented with CAT (Cefoperazone, Amphotericin B Teicoplanin). species isolated using...
The physicochemical characteristics and sensory traits of Cinisara bresaola were investigated, to explore a new commercial opportunity for autochthonous dairy cattle farms. Semimembranosus, Semitendinosus Biceps brachii muscles, from adult cows (AC) grazing (GB) or housed (HB) young bulls breed, processed made bresaola. Differences due animal category muscle type observed. Bresaola AC was richer in fat volatile organic compounds. showed higher colorimetric parameters, and, when animals,...
The present work was carried out to investigate the microbiological profile of Sicilian ewes’ ricotta cheeses during fifteen years investigations (2002-2016). samples were collected between those conferred Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi) Adelmo Mirri, Palermo (Italy), by competent authority official control, food business operator in HACCP systems and research projects. Enterobacteriaceae, Escherichia coli coagulase-positive staphylococci (CPS) found only some...