A5042451483- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Antibiotic Resistance in Bacteria
- Listeria monocytogenes in Food Safety
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Marine Ecology and Invasive Species
- Aquaculture disease management and microbiota
- Milk Quality and Mastitis in Dairy Cows
- Radiation Effects and Dosimetry
- Biosensors and Analytical Detection
- Marine Biology and Environmental Chemistry
- Protein Hydrolysis and Bioactive Peptides
- Bacterial biofilms and quorum sensing
- Dermatological diseases and infestations
- Antimicrobial Resistance in Staphylococcus
- Garlic and Onion Studies
- Marine Toxins and Detection Methods
- Sustainable Urban and Rural Development
- Bartonella species infections research
Istituto Zooprofilattico Sperimentale Della Sicilia
2009-2024
Arcobacter spp. are emerging waterborne and foodborne zoonotic pathogens responsible for gastroenteritis in humans. In this work, we evaluated the occurrence antimicrobial resistance profile of isolates recovered from different aquatic sources. Besides, searched seaweeds corresponding seawater samples. Bacteriological molecular methods applied to 100 samples led isolation 28 27 The highest prevalence was detected rivers followed by artificial ponds, streams, well waters, spring waters....
Salmonella spp. is among the leading causes of foodborne infections in humans and a large number animals. pathogen involved dissemination antimicrobial resistance because it can accumulate antibiotic genes (ARGs). In this study, profile to 15 antibiotics, belonging six different classes, 60 strains collected from pets, farm animals, wildlife, food Sicily (Italy) was investigated by Kirby-Bauer method. Given that almost 33.3% were resistant tetracycline, Real-Time PCR analysis applied on all...
spp. are among the most frequent causes of foodborne diseases, and increasing occurrence MDR strains is an additional cause for concern. In three-year period 2019-2021, we collected
Seafood can vehiculate foodborne illnesses from water to humans. Climate changes, increasing contamination and coastlines anthropization, favor the global spread of Vibrio spp. occurrence antibiotic-resistant isolates. The aim this study was evaluate potentially pathogenic in fishery products collected Sicily assess their antibiotic resistance. Bacteriological molecular methods were applied 603 seafood samples detect V. parahaemolyticus, cholerae, vulnificus, alginolyticus order...
Notification of foodborne outbreaks has been mandatory in Europe since 2005, and surveillance is carried out along the entire food chain. Here we report results obtained from laboratory investigations about four cases that occurred Sicily between 2009 2016, deemed to be related staphylococcal enterotoxins (SEs) coagulase-positive Staphylococci (CPS) by Local Public Health Authority. Primosale cheese samples were processed culture methods for enumeration CPS immunoenzymatic assays detection...
Anisakis spp. and Hysterothylacium are nematodes that commonly parasitize several fish species. Nematode larvae can be recovered in coelomic cavity viscera, but also flesh have an important economic public health impact. A total of 1144 subjects wild teleosts, 340 samples cephalopods 128 specimens farmed collected from Apulia region were analysed for anisakid detection by visual inspection viscera digestion the flesh. No nematode found cephalopod molluscs. All examined wild-caught species...
Borrelia burgdorferi is a bacterial pathogen transmitted by Ixodes ticks and responsible for Lyme disease in both humans dogs. The aim of this work was to evaluate B. diffusion among stray dogs Palermo (Sicily, Italy) serological methods order study the risk factors associated with infection. Serum blood samples 316 were collected from shelter Palermo, analyzed presence antibodies against indirect immunofluorescence assay (IFA), ospA gene real-time PCR, respectively. Seventeen sera (5.4%)...
Bivalves can concentrate biological and chemical pollutants, causing foodborne outbreaks whose occurrence is increasing, due to climatic anthropic factors that are difficult reverse, hence the need for improved surveillance. This study aimed evaluate hygienic qualities of bivalves sampled along production distribution chain in Sicily collect useful data consumer safety. Bacteriological molecular analyses were performed on 254 samples detection enteropathogenic Vibrio, Arcobacter spp.,...
The aim of the study was to evaluate occurrence Arcobacter spp. in food samples collected from Sicilia region. A total 91 products animal origin (41 meat, 17 fresh milk, 18 shellfish) and 15 vegetables, were examined by cultural method confirmed biochemical analysis PCR methods. detection performed, after selective enrichment, on two agar plates: mCCD (modified charcoal cefoperazone deoxycholate) supplemented with CAT (Cefoperazone, Amphotericin B Teicoplanin). species isolated using...
A total of 1104 fish samples from markets Sicily were analysed for the detection and species identification Anisakidae nematodes. The preliminary analysis showed presence 2459 larvae. All revealed different prevalence infestation, with a maximum 100% Lepidopus caudatus minimum 4.5% in Sardina pilchardus. 80% larvae examined by PCR-RFLP belonged to Anisakis pegreffii species. seasonal infestation trend was evaluated all sample examined. results marked connection ecological aspects As far as...
The spread of antibiotic resistance (AR) among zoonotic pathogens is a serious health problem, especially because in the last decade massive use antibiotics has favored emergence Multidrug Resistance (MDR) strains. Some species Salmonella genus are major causes foodborne infections worldwide and could represent reservoirs AR. For these reasons, susceptibility to six classes 63 strains isolated from animals food was determined assess presence MDR In addition, detection genes done for that...
The present work was carried out to investigate the microbiological profile of Sicilian ewes’ ricotta cheeses during fifteen years investigations (2002-2016). samples were collected between those conferred Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi) Adelmo Mirri, Palermo (Italy), by competent authority official control, food business operator in HACCP systems and research projects. Enterobacteriaceae, Escherichia coli coagulase-positive staphylococci (CPS) found only some...
The presence of
Background: Consumption of raw cheese may be associated with different diseases. This study aimed to evaluate behavior four pathogenic bacteria during manufacture and ripening Protected Designation Origin (PDO) Pecorino Siciliano cheese. Methods: The experimental groups were inoculated bacteria, including Escherichia coli O157, Listeria monocytogenes, Salmonella Enteritidis, Staphylococcus aureus. making processes monitored from milk curdling until 3 months ripened cheeses the levels Lactic...
This study shows the frequency of seeds samples contaminated by Salmonella spp. collected randomly from local markets; on 30 black pepper sample no was found while detected in 3 36 (8.3%) analyzed sesame samples; three different serotypes were identified: S. Montevideo, Stanleyville e Tilene. The efficacy gamma irradiation to inactivate Montevideo and irradiated between 1 5 kGy evaluated. is sufficient reduce 3-4 log CFU/g; whereas have been need 5.5-6 CFU/g for sesame. No statistically...
Marine biotoxins can accumulate in filter-feeders bivalve molluscs, that may represent a source of potential health problems being vectors toxins, are transferred to humans through their consumption. Harmful Algal Blooms impact on aquaculture give also economic losses due temporary closures contaminated shellfish harvest and marketing. The presence toxic algae for Paralytic Shellfish Poisoning (PSP), with recurrent blooms dinoflagellates, such as several Alexandrium species, been known since...
Bartonella henselae is a slow growing and facultative intracellular pathogen mainly transmitted by arthropod vectors adapted to domestic wild mammalian reservoir hosts. Since cats are the major source of B. infection, this study aimed evaluate seroprevalence DNA presence in randomly sampled stray cats. Blood samples 429 were collected from shelter Palermo (Southern Italy) sera whole blood analyzed for antibodies against indirect immunofluorescence assay (IFA) real-time polymerase chain...
The traditional cultural method (PCR and Real-Time PCR) for Salmonella spp. detection identification is laborious time-consuming. A qualitative LAMP detecting was validated in compliance with ISO 16140:2016. results show a relative accuracy, sensitivity, specificity of 100% comparison the reference 6579-1:2017; LOD50 set as 0.4 CFU/g. Additionally, field study carried out comparing kit, commercially available PCR kit (FoodProof Salmonella, Biotecon Diagnostics), method. suitable reliable...
This study aimed at detecting the presence of antibiotic-resistant Gram-negatives in samples meals delivered University General Hospital Palermo, Italy. Antibiotic resistant Gram negatives were isolated July-September 2007 ffrom cold dishes and food contact surfaces utensils. Bacterial strains submitted to susceptibility test subtyped by random amplification polymorphic DNA (RAPD). Forty-six 55 (83.6%) 14 17 (82.3%) environmental swabs culture positive for negative bacilli least one group...