A5016150684- Parasite Biology and Host Interactions
- Marine Ecology and Invasive Species
- Identification and Quantification in Food
- Mercury impact and mitigation studies
- Wheat and Barley Genetics and Pathology
- Mycotoxins in Agriculture and Food
- Parasitic infections in humans and animals
- Parasites and Host Interactions
- Listeria monocytogenes in Food Safety
- Plant Pathogens and Fungal Diseases
- Isotope Analysis in Ecology
- Myxozoan Parasites in Aquatic Species
- Polysaccharides and Plant Cell Walls
- Polysaccharides Composition and Applications
- Bird parasitology and diseases
- Marine and fisheries research
- Heavy metals in environment
- Helminth infection and control
- Pesticide Residue Analysis and Safety
- Mollusks and Parasites Studies
- Fish Biology and Ecology Studies
- Environmental Toxicology and Ecotoxicology
- Heavy Metal Exposure and Toxicity
Istituto Zooprofilattico Sperimentale Della Sicilia
2016-2024
We developed and validated a screening method for mycotoxin analysis in cereal products spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) was used the analysis. Dispersive solid-phase extractions (d-SPEs) were extraction of samples. Ochratoxin A (OTA), zearalenone (ZEA), aflatoxins (AFLA; AFB1, AFB2, AFG1, AFG2), deoxynivalenol (DON), fumonisin (FUMO; FB1, FB2, FB3), T2, HT2 maize. AFLA DON black pepper. The satisfies requirements...
The aim of this study was to investigate mercury accumulation in some species, caught Mediterranean Sea, the period between May and December 2015, compare it presence Anisakis parasites. samples were examined by direct analyzer (DMA-80) for their Hg levels. metal concentration compared or absence Significant differences analysed observed. low-infested fishes contained 1–6 larvae parasites whereas high-infested one had 7–83 larvae.
In this study, we investigate the role of microwave cooking in reducing mycotoxin contamination plant-based food matrices, with a focus on veggie burgers (purchased and home-made) their ingredients (soybean, potatoes, zucchini, carrots). Two different conditions were studied (Max–Min) that 800 W for 60 s 90 s, respectively. The degradation patterns aflatoxins (AFB1, AFB2, AFG1, AFG2), fumonisins (FB1, FB2, FB3), trichothecenes (T2, HT2, ZEA), ochratoxin A (OTA) studied. extraction procedures...
In this work a total of 949 fish samples were analysed for the identification nematode larvae belonging to Anisakidae family. Biomolecular application can be an optimal instrument traceability products, described on Reg. EC 178/2002. Results confirm correlation between geographical distribution fishes and presence specific Anisakid larvae. FAO 37 zone (Mediterranean sea) showed prevailing <em>Anisakis pegreffii</em> minimal A. simplex s.s. in hybrid form with...
A total of 1104 fish samples from markets Sicily were analysed for the detection and species identification Anisakidae nematodes. The preliminary analysis showed presence 2459 larvae. All revealed different prevalence infestation, with a maximum 100% Lepidopus caudatus minimum 4.5% in Sardina pilchardus. 80% larvae examined by PCR-RFLP belonged to Anisakis pegreffii species. seasonal infestation trend was evaluated all sample examined. results marked connection ecological aspects As far as...
Background. Diagnosis of anisakis allergy (AA) is based on the skin prick test (SPT) and specific IgE (sIgE) determination.Anyway, false positivity cases are due to cross reactivity with numerous allergens.The aim study was evaluate reliability a comprehensive diagnostic algorithm for AA.Methods.An observational conducted sample consecutive subjects accessing allergology outpatient ambulatories two hospitals located in Western Sicily.All recruited outpatients were tested by Skin Prick Test...
Samples obtained from 11 teleost fish species collected in waters off of the Mediterranean coasts Sicily, Italy, were examined for presence Hysterothylacium spp. larvae. In total, 3,017 samples examined, and larvae recovered used subsequent phylogenetic studies. Fifty-eight raphidascaridid parasitic nematodes found fish, with prevalence values ranging 0.2% anchovies to 60% forkbeard samples. Twenty-seven parasites identified as fabri aduncum by sequencing following regions: nuclear ribosomal...
In this study, four dead great cormorant Phalacrocorax carbo sinensis (Blumenbach, 1978) specimens, collected from the coasts and lakes of Southern Italy, were examined by necropsy for detection Contraceacum sp. The adults larvae found subjected to morphological analysis molecular identification PCR-RFLP. A total 181 Contracaecum specimens detected in all cormorants (prevalence = 100%), showing an intensity infestation between nine ninety-two. co-infestation adult larval forms rudolphii was...
The presence of polyphenols was examined in carob pods (
Aim of the present paper was to report and identify by morphological molecular methods presence anisakid L3 larvae found in 7 specimens Sphoeroides pachygaster caught off Strait Sicily from 2012 2015.Nematode (n=9) were collected three fish samples: morphologically identified as belonging genus Anisakis Type I and, stored 70% ethanol, underwent identification at species level PCR-RFLP analysis rDNA (ITS-1, 5.8S gene, ITS-2) region.Sequencing ITS regions comparison with sequences GenBank also...
In this work a total of 1331 fish samples belonging to 15 species (Engraulis encrasicolus, Alaccia aurita, Loligo vulgaris, Trigla lyra, Conger conger, Merluccius merluccius, Zeus faber, Lophius piscatorius, Sardina pilchardus, Lepidopus caudatus, Scorpaena scrofa, Scomber scombrus, Trachurus trachurus, Todarodes sagittatus, Trachinus draco) from Sicilian commercialized in Sicily (FAO 37, FAO 37.1.3,FAO 37.1.1,FAO 37.2.2, 37.3, FAO27,FAO 41, FAO87), were examined for the detection Anisakidae...
The present work, carried out at the Istituto Zooprofilattico Sperimentale della Sicilia "A.Mirri", Department of Food, Laboratory C.Re.NA (National Reference Center for Anisakiasis), aims to identify species larvae found in different fish from pelagic and benthic environment through molecular analysis (PCR, PCR-RFLP sequencing) evaluate possible ecological relationship between parasites guests two marine environments.
Heavy metals can be essential, benefits and toxic.The 246 samples of swordfish, tuna bivalve molluscs examined coming from the request private individuals at Control Authorities in order to carry out routine checks or inspections food safety.All elements were determined by an ICP-MS.The level contamination heavy fish species sampled Mediterranean Sea has been investigated as indicator water pollution.This study highlighted presence lead cadmium, especially swordfish samples, but all within...