Marco Francesco Ortoffi

ORCID: 0000-0002-8069-107X
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About
Contact & Profiles
Research Areas
  • Listeria monocytogenes in Food Safety
  • Probiotics and Fermented Foods
  • Meat and Animal Product Quality
  • Identification and Quantification in Food
  • Helicobacter pylori-related gastroenterology studies
  • Aquaculture Nutrition and Growth
  • Breastfeeding Practices and Influences
  • Mycobacterium research and diagnosis
  • Enterobacteriaceae and Cronobacter Research
  • Infant Nutrition and Health
  • Diverse academic and cultural studies
  • Herpesvirus Infections and Treatments
  • HIV Research and Treatment
  • Veterinary medicine and infectious diseases
  • Biosensors and Analytical Detection
  • Tea Polyphenols and Effects
  • Ruminant Nutrition and Digestive Physiology
  • Microbial Inactivation Methods
  • Genomics and Phylogenetic Studies
  • Spectroscopy and Chemometric Analyses
  • Protein Hydrolysis and Bioactive Peptides
  • Plant Pathogenic Bacteria Studies
  • Phytochemicals and Antioxidant Activities
  • Rabbits: Nutrition, Reproduction, Health
  • Agriculture and Rural Development Research

Istituto Superiore di Sanità
2003-2025

National Research Council
2013-2018

Institute of Sciences of Food Production
2013-2018

University of Turin
2004-2016

National Academies of Sciences, Engineering, and Medicine
2012

Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta
2003

Centro Ricerche Produzioni Animali
2003

Listeria monocytogenes is an important foodborne pathogen, markedly persistent even in harsh environments and responsible for high hospitalization mortality rates. The aim of the present study was to detect strains circulating Sicily over a five-year period characterize their antimicrobial resistance profiles. key element this sharing data among various entities involved food control clinical surveillance listeriosis order develop integrated approach pathogen. A total 128 isolates were...

10.3390/pathogens14030294 article EN cc-by Pathogens 2025-03-18

To investigate the genetic relatedness between Lactococcus garvieae strains isolated from fish and dairy samples collected in northern Italy, using random-amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR), Sau-PCR amplified fragment length polymorphism (AFLP).Eighty-one isolates bovine caprine products (n = 53) diseased rainbow trouts other fishes 28) were examined. All methods showed a typeability of 100%, repeatability ranging 84.4% to 97.5% discriminatory powers 0.798...

10.1111/j.1365-2672.2008.03780.x article EN Journal of Applied Microbiology 2008-03-12

The effects of three concentrations (0.2%, 1% and 3%) rosemary oil (RO) on the freshness indicators, oxidative stability, fatty acid biogenic amine (BA) contents minced rainbow trout muscle (MTM) were investigated after different periods storage (three nine days) at 4 ± 1 °C. Moreover, terpene sesquiterpene in treated MTM also measured. RO treatment improves pH, stability lipids FA profile, which resulted a significant extension shelf-life. Storage time influenced all with exception...

10.3390/foods1010028 article EN cc-by Foods 2012-12-04

Changing eating habits and rising demand of food have increased the incidence foodborne diseases, particularly in industrialized countries. In this context, contaminated ready-to-eat (RTE) may be a vehicle for transmission Listeria monocytogenes (L. monocytogenes), pathogen responsible listeriosis, severe infectious disease involving humans animals. It would useful to rapid detection methods screen presence L. food. study, colorimetric Loop-mediated isothermal amplification (LAMP) assay was...

10.3390/foods12010193 article EN cc-by Foods 2023-01-01

The presence of enteric Helicobacter species was investigated in poultry (n=130) and pet ornamental birds (n=50) using a PCR sequencing method which permits the differentiation many Helicobhacter derived from animal tissues. All samples were Italian origin, except for 21 Guinea fowl French flock. About 80% (chickens, laying hens, fowl) positive to DNA. H. pullorum most frequently (62.1%) identified whereas pylori 3 sp. hamster B strains seen only cases each. Pet all negative. canadensis...

10.1024/0036-7281.149.9.403 article EN Schweizer Archiv für Tierheilkunde 2007-08-29

ABSTRACT Three recombinant antigens of Leishmania chagasi (= L. infantum ) were expressed in prokaryotic systems and evaluated (using a panel dog sera characterized by parasitological serological immunofluorescent antibody test [IFAT] techniques) as diagnostic markers infection. The whole open reading frame encoding K9, the gene fragment repetitive sequence K26, 3′-terminal single 39-amino-acid subunit kinesin-related protein K39 (K39sub) amplified from DNA cloned into pGEX-2T expression...

10.1128/cdli.10.6.1153-1156.2003 article EN Clinical and Vaccine Immunology 2003-11-01

The use of naturally derived antioxidants to produce ready-to-cook fish-based foods is a growing trend. Grape pomace rich source phenolic compounds with antioxidant and antimicrobial effects. effects red grape (RGP) extracts on the shelf life minced rainbow trout muscles were evaluated. Extracts added patties give final concentration 0, 1, 3%. RGP effective in delaying lipid oxidation cadaverine formation muscle after 6 days refrigerated storage. It concluded that extract can enhance quality...

10.1080/10498850.2013.789094 article EN Journal of Aquatic Food Product Technology 2014-02-21

This study was aimed at collecting data on presence, dissemination and persistence of <em>Pseudomonas</em> in small-scale dairy farms. Six farms (located Piedmont) were visited three times over 2014: 116 waters (wells different faucets/pipes) 117 environmental samples (milking equipments drains) collected. Enumeration <em>Pseudomonadaceae</em> performed, 3-5 colonies/samples selected for identification via 16SrDNA/oprI polymerase chain reaction (PCR), typed by...

10.4081/ijfs.2016.5652 article EN cc-by-nc Italian Journal of Food Safety 2016-05-02

The study investigated the effects of two levels green tea extract (GTE) on common quality parameters and lipid oxidation tench fillets for up to 7 days refrigerated (+2°C) 45 frozen storage (−25°C). Sixty-eight (93.2 ± 25.7 g) were killed by live chilling (water : ice ratio = 1 1; T 2–4°C) then filleted. Four set aside (not treated, NT) while remaining divided into four groups which treated with a sprayed-on solution of: distilled water/ethanol 95% v/v mixture (control group, C); ascorbic...

10.1111/jai.12427 article EN Journal of Applied Ichthyology 2014-05-24

Listeria monocytogenes is one of the most important foodborne pathogens responsible for listeriosis, a severe disease with symptoms ranging from septicemia, meningoencephalitis, and abortion. Given strong impact listeriosis on human health difficulty controlling L. along food production chain, has become priority subjected to molecular surveillance in European Union/European Economic Area since 2007. From 2018, based whole-genome sequence using core genome multilocus type. The complete...

10.3389/fmicb.2022.816880 article EN cc-by Frontiers in Microbiology 2022-04-01

Abstract BACKGROUND The quality of marinated ready‐to‐eat (RTE) swordfish fillets, with or without inoculation the probiotic strain Lactobacillus paracasei IMPC 2.1, was assessed over 3 months refrigerated storage at 4 °C. RTE and control fish fillets were sampled after 7, 14, 30, 60, 90 days storage. Microbiological tests performed, fatty acid (FA) profiles malondialdehyde content examined. counts, including total viable count, lactic bacteria (LAB), yeasts, moulds, Enterobacteriaceae,...

10.1002/jsfa.9161 article EN Journal of the Science of Food and Agriculture 2018-05-31
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