A5015115733- Botulinum Toxin and Related Neurological Disorders
- Streptococcal Infections and Treatments
- Diphtheria, Corynebacterium, and Tetanus
- Hereditary Neurological Disorders
- Neurological disorders and treatments
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Plant-based Medicinal Research
- Microbial Inactivation Methods
- Listeria monocytogenes in Food Safety
- Biosensors and Analytical Detection
- Microbial Metabolism and Applications
- Neurological diseases and metabolism
- Global Energy and Sustainability Research
- Healthcare and Venom Research
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Agricultural safety and regulations
- Complementary and Alternative Medicine Studies
- Hepatitis Viruses Studies and Epidemiology
- Vibrio bacteria research studies
- Molecular Biology Techniques and Applications
- Parkinson's Disease Mechanisms and Treatments
- Bacterial biofilms and quorum sensing
Istituto Superiore di Sanità
2010-2021
Swedish Veterinary Agency
2013
Istituto Zooprofilattico Sperimentale delle Venezie
2013
Swedish University of Agricultural Sciences
2013
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2013
Botulism is a rare but severe neuroparalytic disease caused by botulinum toxins. Because of its high potential impact on public health, botulism closely monitored communicable in Europe. In Italy, which has one the highest incidence rates Europe (0.03 cases per 100,000 population), through case-based passive surveillance system: front-line physician who diagnoses suspected case must notify Local Health Units immediately, and Ministry Health's office within 12 hours. From 1986 to 2015, 466...
Botulism is a severe neuroparalytic disease that affects humans, all warm-blooded animals, and some fishes. The caused by exposure to toxins produced Clostridium botulinum other toxin-producing clostridia. in animals represents environmental economic concern because of its high mortality rate. Moreover, meat or products from affected entering the food chain may result public health problem. To this end, early diagnosis crucial define apply appropriate veterinary measures. Clinical based on...
Listeria monocytogenes is frequently found as a contaminant in raw and ready-to-eat foods. The ability of L. to multiply at refrigeration temperatures grow wide range pH values particular concern for food safety. According the European Union regulation on microbiological criteria foodstuffs, must be absent some categories standard method detection foods (ISO 11290-1: 1996 (ISO, International Organization Standardization)) cost time consuming. Developments rapid, cost-effective automated...
Of the seven currently known botulinum neurotoxin-producing species of Clostridium , C. parabotulinum, or Group I, is associated with majority human botulism cases worldwide. Phylogenetic analysis these bacteria reveals a diverse multiple genomic clades. The neurotoxins they produce are also diverse, over 20 subtypes represented. existence different bont genes within very similar genomes and same genes/gene clusters bacterial variants/species indicates that have evolved independently....
Botulism is a neuroparalytic disease that can occur in all warm-blooded animals, birds, and fishes. The animals mainly caused by toxins produced Clostridium botulinum strains belonging to group III, although outbreaks due I II organisms have been recognized. Group III are capable of producing type C, D, C/D D/C mosaic variants. Definitive diagnosis animal botulism made combining clinical findings with laboratory investigations. Detection specimens feed the gold standard for diagnosis. Since...
We report the development of real-time PCR assays for genotyping Clostridium botulinum group III targeting newly defined C. novyi sensu lato group; nontoxic nonhemagglutinin (NTNH)-encoding gene ntnh; neurotoxin (BoNT)-encoding genes bont/C, bont/C/D, bont/D, and bont/D/C; flagellin (fliC) gene. The genetic diversity fliC among strains resulted in definition five major subgroups named fliC-I to fliC-V. Investigation subtypes 560 samples, with various European origins, showed that was...
Sufficient quality and quantity of extracted DNA is critical to detecting performing genotyping Clostridium botulinum by means PCR-based methods. An ideal extraction method has optimize yield, minimize degradation, allow multiple samples be extracted, efficient in terms cost, time, labor, supplies. Eleven toxin-producing clostridia strains 25 (10 food, 13 clinical, 2 environmental samples) naturally contaminated with were used compare 4 procedures: Chelex(®) 100 matrix,...
Botulism in cattle is rarely reported Italy. This study describes an outbreak of botulism a dairy herd Central Italy September 2012, and the notably high mortality rate it caused. Differential diagnoses involving toxicology bacteriology, electrolyte imbalances, all proved negative. A multiplex polymerase chain reaction (PCR) for detecting BoNT gene led to identification causative agent as Clostridium botulinum type DC. The presence toxin was confirmed subsequently via mouse bioassay....
In Italy, foodborne botulism is a rare disease mainly due to home-preserved food. the case reported here, clinical diagnosis was performed on basis of signs and referred consumption turnip tops in oil. Definitive by detection botulinum toxin sera neuro-toxigenic organisms stools leftover This report highlights need high medical awareness, prompt diagnosis, synergic collaboration among health authorities for correct management as well containment.
Here, we report the draft genome sequence of Clostridium botulinum B2 450, responsible for first reported case wound botulism in a drug user Italy.