A5034328234- Analytical Chemistry and Chromatography
- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- Hormonal and reproductive studies
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects and Assays
- Antibiotics Pharmacokinetics and Efficacy
- Mass Spectrometry Techniques and Applications
- Muscle metabolism and nutrition
- Pulmonary Hypertension Research and Treatments
- Microfluidic and Capillary Electrophoresis Applications
- Botulinum Toxin and Related Neurological Disorders
- Drug-Induced Hepatotoxicity and Protection
- Analytical Methods in Pharmaceuticals
- Veterinary Equine Medical Research
- Microbial Natural Products and Biosynthesis
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer, Lipids, and Metabolism
- Pharmacology and Obesity Treatment
- Drug Solubulity and Delivery Systems
- Veterinary Pharmacology and Anesthesia
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mycorrhizal Fungi and Plant Interactions
- Metabolism and Genetic Disorders
- Pharmacological Effects and Toxicity Studies
Uppsala University
2015-2024
Swedish Veterinary Agency
2012-2021
Medical Products Agency
2019
Istituto Superiore di Sanità
2013
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2013
Botulism is a severe neuroparalytic disease that affects humans, all warm-blooded animals, and some fishes. The caused by exposure to toxins produced Clostridium botulinum other toxin-producing clostridia. in animals represents environmental economic concern because of its high mortality rate. Moreover, meat or products from affected entering the food chain may result public health problem. To this end, early diagnosis crucial define apply appropriate veterinary measures. Clinical based on...
Cytostatic effects of doxorubicin in clinically applied doses are often inadequate and limited by systemic toxicity. The main objective this vitro study was to determine the anti-tumoral effect (IC50) intracellular accumulation free liposomal (DOX) four human cancer cell lines (HepG2, Huh7, SNU449 MCF7). results showed a correlation between longer DOX exposure time lower IC50 values, which can be attributed an increased cellular uptake DOX, ultimately leading death. We found that total...
Chemically induced, targeted protein degradation with proteolysis targeting chimeras (PROTACs) has shown to be a promising pharmacological strategy circumvent the poor "druggability" of intracellular targets. However, favorable pharmacology comes complex molecular properties limiting oral bioavailability these drugs. To foster translation PROTACs into clinics it is high importance establish sensitive bioanalytical methods that enable assessment absorption, bioavailability, and disposition...
Selective androgen receptor modulators, SARMs, constitute a class of compounds with anabolic properties but few androgenic side‐effects. This makes them possible substances abuse and the World Anti‐Doping Agency (WADA) has banned entire substances. There have been several cases illicit use aryl propionamide SARMs in human sports 2013, 13 were reported. These found to be extensively metabolized humans, making detection metabolites necessary for doping control. are also great interest equine...
β-N-Methylamino-l-alanine (BMAA) is a potential neurotoxin associated with the aquatic environment. Validated analytical methods for quantification of both free and total concentrations BMAA were used in an investigation seafood purchased from different grocery stores Uppsala, Sweden. The analysis was performed using ultra high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI–MS/MS) detection as dansyl derivate. determined (after simple...
Rationale Potentially performance‐enhancing agents, particularly anabolic are advertised and distributed by Internet‐based suppliers to a substantial extent. Among these substance referred as LGD‐4033 has been made available, comprising the core structure of class selective androgen receptor modulators (SARMs). Methods In order provide comprehensive analytical data for doping controls, was obtained characterized nuclear magnetic resonance spectroscopy (NMR) liquid chromatography/electrospray...
Doxorubicin (DOX) delivered in a lipiodol-based emulsion (LIPDOX) or drug-eluting beads (DEBDOX) is used as palliative treatment patients with intermediate-stage hepatocellular carcinoma (HCC). The primary objective of this study was to evaluate the vivo delivery performance DOX from LIPDOX DEBDOX HCC using local and systemic pharmacokinetics its main metabolite doxorubicinol (DOXol). Urinary excretion DOXol their short-term safety antitumor effects were also evaluated. In open, prospective,...
A new metabolic scheme of flutamide is proposed in this article. Some patients treated with flutamide, a nonsteroidal antiandrogen, have developed severe hepatic dysfunction. Toxic metabolites been to be responsible for these negative effects. In study, the qualitative aspects vitro metabolism liver microsomes from human, dog, pig, and rat were evaluated. direct comparison prostate pig was made, vivo investigated urine orally cancer patients. Liquid chromatography/tandem mass spectrometry...
Transcellular permeation enhancers are known to increase the intestinal permeability of enalaprilat, a 349 Da peptide, but not hexarelin (887 Da). The primary aim this paper was investigate if paracellular affected two peptides. This investigated using rat single-pass perfusion model with concomitant blood sampling. These luminal compositions included enhancers, chitosan (5 mg/mL) and ethylenediaminetetraacetate (EDTA, 1 5 mg/mL), as well low tonicity (100 mOsm) or without lidocaine. Effects...
Nanocellulose–oligoproline hydrogels, able to respond high levels of ROS and protect cells from oxidative environments, are promising candidates for the treatment chronic wounds other clinical conditions associated with ROS.
The aim of this study in pigs was to investigate the local pharmacokinetics fexofenadine intestine and liver by using pig as a model for drug transport entero-hepatobiliary system. A parallel group design included seven (10–12 weeks, 22.2–29.5 kg) three groups (G1, G2, G3), jejunal single-pass perfusion combined with sampling from bile duct portal, hepatic, superior caval veins performed. Fexofenadine perfused through segment alone (G1: 120 mg/l, total dose 24 mg) or two different verapamil...
A workshop on animal botulism was held in Uppsala, Sweden, June 2012. Its purpose to explore the current status of disease Europe by gathering European experts and raise awareness among veterinarians others involved biopreparedness. Animal is underreported underdiagnosed, but an increasing number reports, as well information gathered from this workshop, show that it emerging problem Europe. The divided into 4 sessions: Europe, bacteria behind disease, detection diagnostics, collaboration...
There have been several outbreaks of botulism among poultry and wild birds in Sweden recent years. The National Veterinary Institute (SVA) has identified botulinum neurotoxin (BoNT)/C1 or the mosaic BoNT/C1D using mouse bioassay. This is believed to be first report on application Endopep mass spectrometry (Endopep-MS) method selected clinical animal (serum liver) samples a feed sample that had previously given positive test results with In bioassay eight eleven were found neutralized by both...
This paper describes the search for procedures through which xenobiotic pollutant diclofenac can be removed from non-sterile aquatic systems. Specifically, adsorption to solid supports (carriers) in combination with biodegradation by non-immobilized and immobilized white rot fungus Trametes versicolor were investigated. Batch experiments using polyurethane foam (PUF)-carriers resulted 99.9% removal after 4 h, monolayer of carrier glass surfaces accounting most decrease. Enzymatic reactions...