A5063509910- Salmonella and Campylobacter epidemiology
- Bacillus and Francisella bacterial research
- Viral gastroenteritis research and epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Botulinum Toxin and Related Neurological Disorders
- Animal Disease Management and Epidemiology
- Microbial infections and disease research
- Poxvirus research and outbreaks
- Clostridium difficile and Clostridium perfringens research
- Diphtheria, Corynebacterium, and Tetanus
- Streptococcal Infections and Treatments
- Veterinary medicine and infectious diseases
- Animal Nutrition and Physiology
- Microbial Inactivation Methods
- Food Safety and Hygiene
- Leptospirosis research and findings
- Listeria monocytogenes in Food Safety
- Gut microbiota and health
- Livestock and Poultry Management
- Identification and Quantification in Food
- Antibiotic Use and Resistance
- Zoonotic diseases and public health
- Viral Infections and Outbreaks Research
- Insect and Pesticide Research
- Neurological disorders and treatments
Wageningen University & Research
2015-2024
Biology of Infection
2019-2022
New York Genome Center
2022
Utrecht University
2004-2022
European Centre for Disease Prevention and Control
2022
National Institute for Public Health and the Environment
2022
Istituto Superiore di Sanità
2013
Istituto Zooprofilattico Sperimentale delle Venezie
2013
We compared Campylobacter jejuni/coli multilocus sequence types (STs) from pets (dogs/cats) and their owners investigated risk factors for pet-associated human campylobacteriosis using a combined source-attribution case-control analysis. In total, 132/687 pet stools were Campylobacter-positive, resulting in 499 strains isolated (320 C. upsaliensis/helveticus, 100 jejuni, 33 hyointestinalis/fetus, 10 lari, 4 coli, 32 unidentified). There 737 104 assigned to 154 49 STs, respectively. Dog,...
Clostridium botulinum types C and D, as well their mosaic variants C-D D-C, are associated with avian mammalian botulism. This study reports on the development of low-density macroarrays based GeneDisc cycler platform (Pall-GeneDisc Technologies) applied to simultaneous detection C. subtypes C, C-D, D-C. The limit PCR assays was 38 fg total DNA, corresponding 15 genome copies. Artificially contaminated samples cecum showed a below 50 spores/g. tests were performed large variety bacterial...
ObjectivesTo determine the contributions of several animal and environmental sources human campylobacteriosis identify source-specific risk factors.Methods1417 Campylobacter jejuni/coli isolates from Netherlands in 2017–2019 were whole-genome sequenced, including cases (n = 280), chickens/turkeys 238), laying hens 56), cattle 158), veal calves 49), sheep/goats 111), pigs 110), dogs/cats 100), wild birds 62), surface water 253). Questionnaire-based exposure data was collected. Source...
Botulism is a severe neuroparalytic disease that affects humans, all warm-blooded animals, and some fishes. The caused by exposure to toxins produced Clostridium botulinum other toxin-producing clostridia. in animals represents environmental economic concern because of its high mortality rate. Moreover, meat or products from affected entering the food chain may result public health problem. To this end, early diagnosis crucial define apply appropriate veterinary measures. Clinical based on...
Campylobacter jejuni is the leading bacterial food-borne pathogen in Europe. Despite accepted limits of cultural detection fastidious bacterium, "gold standard" food microbiology still determination colony-forming units (CFU). As an alternative, a live/dead differentiating qPCR has been established, using propidium monoazide (PMA) as DNA-intercalating crosslink agent for inactivating DNA from dead, membrane-compromised cells. The PMA treatment was combined with addition internal sample...
ABSTRACT The prevalence of coinfection Campylobacter species in dogs was determined using four isolation methods. In 26% the positive-testing stools, multiple were identified. use methods as well time lapse between sampling and processing are important for detection coinfection.
A workshop on animal botulism was held in Uppsala, Sweden, June 2012. Its purpose to explore the current status of disease Europe by gathering European experts and raise awareness among veterinarians others involved biopreparedness. Animal is underreported underdiagnosed, but an increasing number reports, as well information gathered from this workshop, show that it emerging problem Europe. The divided into 4 sessions: Europe, bacteria behind disease, detection diagnostics, collaboration...
Bacillus anthracis, the causative agent of anthrax, is a zoonotic pathogen that relatively common throughout world and may cause life threatening diseases in animals humans. There are many PCR-based assays use for detection B. anthracis. While most developed rely on unique markers present virulence plasmids pXO1 pXO2, few incorporate chromosomal DNA due to close relatedness anthracis cereus group strains. For DNA, different genes have been used, such as BA813, rpoB, gyrA, plcR, S-layer,...
Bacillus anthracis, the causative agent of anthrax, has been widely described as a clonal species. Here we report use both canonical SNP analysis and whole-genome sequencing to characterize phylogenetic lineages B. anthracis from Netherlands. Eleven strains isolated over 25-years period (1968-1993) were paired-end sequenced using parallel technology. Five canSNP groups or lineages, i.e. A.Br.001/002 (n=6), A.Br.Aust94 (n=2), A.Br.008/011 (n=1), A.Br.011/009 (n=1) A.Br.Vollum identified....
In this study we evaluated the RespoCheck Mycoplasma triplex real-time PCR for detection in bronchoalveolar lavage fluid (BALF) of (M.) dispar, M. bovis and bovirhinis, all three associated with bovine respiratory disease (BRD). Primers probes are based on V3/V4 region 16S rRNA gene species. The analytical sensitivity was, as determined by spiking experiments strains Phosphate Buffered Saline, 300 colony forming units (cfu)/mL 30 cfu/mL or bovirhinis. PCRwas, purified DNA, 10 fg DNA per...
Sequence-based typing of Francisella tularensis has led to insights in the evolutionary developments tularemia. In Europe, two major basal clades F. subsp. holarctica exist, with a distinct geographical distribution. Basal clade B.6 is primarily found Western while B.12 occurs predominantly central and eastern parts Europe. There are indications that tularemia geographically expanding strains from might differ pathogenicity, being potentially more virulent than B.12. This study provides...
Although most infections are transmitted through the environment, processes underlying environmental stage of transmission still poorly understood for systems. Improved understanding dynamics is important effective non-pharmaceutical intervention strategies. To study mechanisms we formulated a parsimonious modelling framework including hypothesised pathogen dispersion and decay. calibrate validate model, conducted series experiments studying distance-dependent Campylobacter jejuni in...
Contamination of recreational water by bird feces is a main concern managers. It important to understand the sources Escherichia coli contamination since organism frequently used as hygiene parameter. Here, we address presence and levels E. in fecal shedding from several waterfowl (25 geese, 20 coots, 40 gulls) demonstrate that there species variation. Results indicate gull contain greater average concentration per gram than do geese or coot feces. However, risks also depends on abundance....
In veterinary medicine and animal husbandry, there is a need for tools allowing the early warning of diseases. Preferably, tests should be available that warn farmers veterinarians during incubation periods disease before onset clinical signs. The objective this study was to explore potential serum protein profiles as an biomarker infectious status. Serum samples were obtained from experimental pig model porcine circovirus-associated (PCVAD), consisting Porcine Circovirus type 2 (PCV2)...
Tularaemia, a disease caused by the bacterium Francisella tularensis , is re-emerging zoonosis in Netherlands. After sporadic human and hare cases occurred period 2011 to 2014, cluster of F. -infected hares was recognised region north Netherlands from February May 2015. No were identified, including after active case finding. Presence investigated potential reservoirs transmission routes, common voles, arthropod vectors surface waters. not detected mosquito larvae or adults, tabanids ticks....
Background: Brown rats (Rattus norvegicus) may carry pathogens that can be a risk for public health. in the Netherlands were tested zoonotic Leptospira spp. and Seoul hantavirus (SEOV), order to obtain insight their prevalence. Methods results: Cross-sectional studies performed at four locations from 2011 2015. The using real-time PCR and/or culture resulting prevalence ranging between 33–57%. Testing SEOV was done through an adapted human ELISA RT-PCR. Although several indicated presence of...
Tularaemia has not been reported in Dutch wildlife since 1953. To enhance detection, as of July 2011, brown hares (Lepus europaeus) submitted for post-mortem examination the context non-targeted disease surveillance, were routinely tested tularaemia by polymerase chain reaction (PCR). Francisella tularensis subspecies holarctica infection was confirmed a hare May 2013. The case occurred Limburg, near site 1953 case. Further surveillance should clarify significance this finding.
Pasteurella multocida, Mannheimia haemolytica, Histophilus somni and Trueperella pyogenes are four bacterial agents commonly associated with bovine respiratory disease (BRD). In this study a multiplex real-time PCR (the RespoCheck PCR) was evaluated for the detection in bronchoalveolar lavage fluid (BALF) of these agents. The analytical sensitivity assay determined on purified DNA cells target pathogens one to ten fg DNA/assay 4 × 10−1 2 100 CFU/assay. specificity test was, as collection 118...
Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the in livestock and human populations. The primary aim of this study was to assess presence Brucella pinnipedialis mammals living along Dutch coast observe possible correlation between B. accompanying pathology found infected animals. overall prevalence spp. antibodies sera from healthy wild grey seals (Halichoerus grypus; n=11) harbor (Phoca vitulina; n=40), collected 2007 2013 ranged...