A5060324823- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Outbreaks Research
- Reproductive tract infections research
- Leptospirosis research and findings
- Fire effects on ecosystems
- Urinary Tract Infections Management
- Zoonotic diseases and public health
- Bartonella species infections research
- Syphilis Diagnosis and Treatment
- Parasitic Infections and Diagnostics
- Urinary and Genital Oncology Studies
- Animal Disease Management and Epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Amoebic Infections and Treatments
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Dengue and Mosquito Control Research
- Reproductive Physiology in Livestock
- Silymarin and Mushroom Poisoning
- Veterinary medicine and infectious diseases
- Infective Endocarditis Diagnosis and Management
- Legionella and Acanthamoeba research
National Institute for Public Health and the Environment
2016-2025
Amsterdam University Medical Centers
2024
Wageningen University & Research
2008-2022
Sciensano (Belgium)
2019-2022
Utrecht University
2019
Rijnstate Hospital
2018
Veiligheids- en Gezondheidsregio Gelderland-Midden
2018
Netherlands Food and Consumer Product Safety Authority
2018
Friedrich-Loeffler-Institut
2012
Bernhard Nocht Institute for Tropical Medicine
2012
ABSTRACT From 2000 to 2004, ticks were collected by dragging a blanket in four habitat areas The Netherlands: dunes, heather, forest, and city park. Tick densities calculated, infection with Borrelia burgdorferi Anaplasma Ehrlichia species was investigated reverse line blot analysis. lowest tick density observed the heather area (1 8/100 m 2 ). In oak forest park, ranged from 26 45/100 . highest found dune (139 551/100 rates varied significantly for study years, ranging 0.8 11. 5% spp. 1 16%...
To test the hypothesis that chronic infection with Chlamydia pneumoniae , Helicobacter pylori or cytomegalovirus is associated coronary heart disease risk in elderly men, a nested case-control study cohort investigated 1985 and 1990 town of Zutphen, The Netherlands, was designed. Fifty-four cases first diagnosed event between 1990, 108 age-matched control subjects free during follow up were included study. overall prevalence antibodies to 74·7%, H. 75·9% C. 84·0%. A high level an increased...
In July 2016, the first autochthonous case of tick-borne encephalitis was diagnosed in Netherlands, five days after a report that virus (TBEV) had been found Dutch ticks. A person their 60s without recent travel history suffered from neurological symptoms tick bite. TBEV serology positive and qRT-PCR. infection should be considered patients with compatible Netherlands.
We report the presence of tick-borne encephalitis virus (TBEV) in Netherlands. Serologic screening roe deer found TBEV-neutralizing antibodies with a seroprevalence 2%, and TBEV RNA was detected 2 ticks from same location. Enhanced surveillance awareness among medical professionals has led to identification autochthonous cases.
Urban greening has benefits for both human and environmental health. However, urban might also have negative effects as the abundance of wild rats, which can host spread a great diversity zoonotic pathogens, increases with greenness. Studies on effect rat-borne pathogens are currently unavailable. Therefore, we investigated how greenness is associated pathogen prevalence diversity, translated this to disease hazard. We screened 412 rats (Rattus norvegicus Rattus rattus) from three cities in...
Fecal samples of 211 bats representing 13 different bat species from 31 locations in the Netherlands were analyzed for presence coronaviruses (CoV) using a genus-wide reverse transcription (RT)-polymerase chain reaction. CoVs are known their high potential interspecies transmission, including zoonotic transmission with as reservoir hosts. For first time, group 2 CoV was found bat, Pipistrellus pipistrellus, Europe. This is particular interest public health host that common to urban areas...
In the summer of 2005, Asian tiger mosquito, Aedes albopictus (Skuse) (Diptera: Culicidae) was found for first time in Netherlands. It collected on premises several horticultural companies that import ornamental plant Dracaena sanderiana (Sparagalus: Dracaenaceae [Agavaceae]), known as Lucky bamboo, from southern China, an area endemic this mosquito species and arboviruses transmitted by vector. Here we report results a 1-year survey distribution vector status Ae. bamboo nurseries...
Abstract Tick-borne encephalitis virus (TBEV) is an emerging pathogen that was first detected in ticks and humans the Netherlands 2015 (ticks) 2016 (humans). To learn more about its distribution prevalence Netherlands, we conducted large-scale surveillance rodents during August 2018–September 2020. We tested 320 wild >46,000 from 48 locations considered to be at high risk for TBEV circulation. found RNA 3 (0.9%) 7 tick pools (minimum infection rate 0.02%) 5 geographically distinct foci....
Background Tick-borne encephalitis (TBE) can be a severe neurological disease. Identifying ecological factors that may facilitate tick-borne virus (TBEV) circulation in the Netherlands could improve awareness and detection. Aim We aimed to identify affecting TBEV determine if there is sustained spread of virus. Methods Between June September 2021, rodents ticks from three previously TBEV-positive locations were tested for by PCR. sequenced compared sequences with previous subsequent other...
Serological assays are important tools for detecting the presence of antibodies that indicative past and ongoing infections. For wildlife, species-specific conjugates, which used as detection in primary binding assays, not available most species. In these cases, conjugates closely related species or immunoglobulin-binding proteins frequently used. These often validated their low functional affinity may result false-negative results. We tested 11 commercial including protein G species-...
Mycoplasma contamination of biological materials remains a major problem. Most contaminations are caused by the use Mycoplasma-contaminated cell lines. We adapted group-specific PCR to detect in lines and demonstrate its monitoring decontamination procedures with suspensions Chlamydia spp. Three different methods were investigated: Mycoplasma-specific antiserum culture, physical separation combined enzymatic treatment differential centrifugation, detergents. With these only incubation Triton...
The emergence of Schmallenberg virus (SBV), a novel orthobunyavirus, in ruminants Europe triggered joint veterinary and public health response to address the possible consequences human health. Use risk profiling algorithm enabled conclusion that for zoonotic transmission SBV could not be excluded completely. Self-reported problems were monitored, serologic study was initiated among persons living and/or working on SBV-affected farms. In set-up, we addressed vector direct routes putative...
Usutu virus (USUV) is an emerging flavivirus that maintained in enzootic cycle with mosquitoes as vectors and birds amplifying hosts. In Europe, the has caused mass mortality of wild birds, mainly among Common Blackbird ( Turdus merula ) populations. While are primary for USUV, Blackbirds other avian species exposed to arthropod ectoparasites, such ticks. It unknown, however, if ticks can maintain transmit USUV. We addressed this question using vitro vivo experiments field collected data....
Mosquitoes (Diptera: Culicidae) are the principal vectors of West Nile virus (WNV). The likely introduction pathway WNV into northwestern Europe is by infected migratory birds from WNV-endemic regions like Africa and central Europe. For efficient local enzootic transmission, mosquito species required that capable sustaining transmitting among indigenous bird population. Ornithophilic most relevant for establishment in an cycle (Hubalek Halouzka 1999, Koopmans et al. 2008, Rappole 2000,...
Seoul orthohantavirus (SEOV) is a rat-associated zoonotic pathogen with an almost worldwide distribution. In 2019, the first autochthonous human case of SEOV-induced hemorrhagic fever renal syndrome was reported in Germany, and pet rat identified as source infection. To further investigate SEOV reservoir, additional rats from patient another owner, all which were purchased same vendor, tested. RNA anti-SEOV antibodies found both patient's two three belonging to other owner. The complete...
Abstract —In this article, we describe the results of a comparative study for detection Chlamydia pneumoniae in abdominal aortic aneurysm specimens 19 patients through use immunocytochemistry (ICC), situ hybridization (ISH), and polymerase chain reaction (PCR), along with cytomegalovirus (CMV) herpes simplex virus (HSV) by ICC PCR. C –specific membrane protein was detected all (100%; 95% confidence interval [CI] 82% to 100%) 15 (79%; CI 54% 94%) monoclonal antibodies RR-402 TT-401,...
Abstract Orthohantaviruses are a group of rodentborne viruses with worldwide distribution. The orthohantavirus Seoul virus (SEOV) can cause hemorrhagic fever renal syndrome in humans and is distributed worldwide, like its reservoir host, the rat. Cases SEOV wild pet rats have been described several countries, human cases reported United Kingdom, France, Canada, States. In Netherlands, has previously found brown rats. We describe an autochthonous case infection Netherlands. This patient had...
Tick-borne encephalitis virus (TBEV) may cause tick-borne (TBE), a potential life-threatening infection of the central nervous system in humans. Phylogenetically, TBEVs can be subdivided into three main subtypes, which differ endemic region and pathogenic potential. In 2016, TBEV was first detected Netherlands. One two strains, referred to as Salland, belonged TBEV-Eu subtype, yet diverged ≥ 2% on amino acid level from other members this subtype. Here, we report successful rescue strain...
The genetic diversity of the 16S-23S rRNA spacer region 12 Chlamydia pneumoniae isolates, 7 trachomatis isolates (human biovars: trachoma serovars B and C, urogenital D, E, F, lymphogranuloma venereum serovar L2; a mouse biovar), 6 psittaci (5 avian 1 feline isolate), one pecorum isolate was studied. amplified by PCR digested with restriction enzymes MseI, PstI, AluI, BglII, NlaIV, BclI. All 26 could be using genus-specific primer pair, yielding an amplicon size 803 bp. analytical...
A panel of monoclonal antibodies was developed for serovar typing clinical isolates Chlamydia trachomatis. The could distinguish all 15 established serovars from one another, although the hybridomas were by fusions myeloma cells and spleen mice immunized with antigen derived urogenital D to L3. assay based on a dot enzyme immunoassay, that included in reacted strongly this assay. collection 289 Netherlands typed. observed frequency distribution 51 (17.6%), 103 E (35.6%), 62 F (21.5%), 28 G...
Abstract To increase knowledge of tick-borne encephalitis virus (TBEV) circulation in the Netherlands, we conducted serosurveillance roe deer (Capreolus capreolus) during 2017 and compared results with those obtained 2010. Results corroborate a more widespread occurrence 2017. Additional precautionary public health measures have been taken.