A5035302734- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- Reproductive tract infections research
- Microbial infections and disease research
- Mycotoxins in Agriculture and Food
- Antifungal resistance and susceptibility
- Zoonotic diseases and public health
- Education, sociology, and vocational training
- Bacteriophages and microbial interactions
- Advanced Numerical Analysis Techniques
- Manufacturing Process and Optimization
- Computational Geometry and Mesh Generation
- Identification and Quantification in Food
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Robotic Mechanisms and Dynamics
- Logic, Reasoning, and Knowledge
- Coccidia and coccidiosis research
- Logic, programming, and type systems
- Infectious Diseases and Mycology
- Bacterial Genetics and Biotechnology
- Genetic and phenotypic traits in livestock
- Clostridium difficile and Clostridium perfringens research
- Constraint Satisfaction and Optimization
- Educational Practices and Policies
Milieux environnementaux, transferts et interactions dans les hydrosystèmes et les sols
2020-2024
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2008-2017
Département Santé Animale
2011-2016
École Nationale Vétérinaire d'Alfort
2011-2014
STMicroelectronics (Czechia)
2014
Paris-Est Sup
2013
Bipar
2011-2012
Université de Strasbourg
2009-2011
Centre National de la Recherche Scientifique
2007-2011
Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie
2010
Aspergillus fumigatus remains a major respiratory pathogen in birds. In poultry, infection by A. may induce significant economic losses particularly turkey production. develops and sporulates easily poor quality bedding or contaminated feedstuffs indoor farm environments. Inadequate ventilation dusty conditions increase the risk of bird exposure to aerosolized spores. Acute cases are seen young animals following inhalation spores, causing high morbidity mortality. The chronic form affects...
Background Bacillus anthracis is known to have low genetic variability. In spite of this lack diversity, multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) and single nucleotide polymorphisms (SNPs) including the canonical SNPs assay (canSNP) proved be highly effective differentiate strains. Five different MLVA schemes based on a collection 31 VNTR loci (MLVA8, MLVA15, MLVA20, MLVA25 MLVA31) with increased resolving power been described. Results MLVA31 was applied...
Background Bacillus anthracis, the highly dangerous zoonotic bacterial pathogen species is currently composed of three genetic groups, called A, B and C. Group A represented worldwide whereas group present essentially in Western Europe Southern Africa. Only strains from C have been reported. This knowledge derived genotyping more than 2000 collected worldwide. Strains both are France. Previous investigations showed that majority sporadic French belong to so-called A.Br.011/009 clade define a...
Anaplasma phagocytophilum is a tick-borne intragranulocytic alpha-proteobacterium. It the causative agent of fever in ruminants, and human granulocytic anaplasmosis humans, two diseases which are becoming increasingly recognized Europe USA. However, while several molecular typing tools have been developed over last years, few them appropriate for in-depth exploration epidemiological cycle this bacterium. Therefore we Multiple-Locus Variable number tandem repeat (VNTR) Analysis technique A....
Bacillus anthracis, the causative agent of anthrax, is a zoonotic pathogen that relatively common throughout world and may cause life threatening diseases in animals humans. There are many PCR-based assays use for detection B. anthracis. While most developed rely on unique markers present virulence plasmids pXO1 pXO2, few incorporate chromosomal DNA due to close relatedness anthracis cereus group strains. For DNA, different genes have been used, such as BA813, rpoB, gyrA, plcR, S-layer,...
Single Nucleotide Polymorphisms (SNPs) are the most common and abundant genetic variation found in genome of any living species, from bacteria to humans. In bacterial genotyping, these evolutionarily stable point mutations represent important DNA markers that can be used elucidate deep phylogenetic relationships among worldwide strains, but also discriminate closely related strains. With advent next generation sequencing (NGS) technologies, affordable solutions now available get access...
Azole resistance in the fungal pathogen Aspergillus fumigatus is an emerging problem and may develop during azole therapy humans animals or exposure to fungicides environment. To assess potential risk of azole-resistance emergence avian farms where compounds are used for control mycoses, we conducted a drug susceptibility study including A. isolates from birds France Southern China. A total number 175 were analyzed: 57 collected chemoprophylaxis with parconazole was performed; 51 southern...
Abstract Background Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) is a prominent subtyping method to resolve closely related microbial isolates provide information for establishing genetic patterns among and investigate disease outbreaks. The usefulness of MLVA was recently demonstrated the avian major pathogen Chlamydophila psittaci . In present study, we developed similar another birds: filamentous fungus Aspergillus fumigatus Results We selected 10 VNTR markers...
Using high-resolution melting (HRM) analysis, we developed a cost-effective method to genotype set of 13 phylogenetically informative single-nucleotide polymorphisms (SNPs) within the genome Bacillus anthracis. SNP discrimination assays were performed in monoplex or duplex and applied 100 B. anthracis isolates collected France from 1953 2009 few reference strains. HRM provided reliable cheap alternative subtype into one 12 major sublineages subgroups. All strains could be correctly...
Aspergillus flavus is second only to A. fumigatus in causing invasive aspergillosis and it the major agent responsible for fungal sinusitis, keratitis endophthalmitis many countries Middle East, Africa Southeast Asia. Despite growing challenge due flavus, data on molecular epidemiology of this fungus remain scarce. The objective present study was develop a new typing method based detection VNTR (Variable number tandem repeat) markers. Eight markers located 6 different chromosomes (1, 2, 3,...
Enteric infections caused by Chlamydia (C.) psittaci are frequent in ducks, but mostly remain subclinical under field conditions. To emulate natural infection, we investigated the pathogenic potential of a C. strain orally inoculated 4-day-old ducklings. Three different challenge doses were tested and seven contact animals also mock-inoculated with buffer each group. Over course ten days, birds monitored for clinical symptoms chlamydial dissemination before final examination tissues using...
This paper deals with the resolution of geometric constraint systems encountered in CAD-CAM. The main results are that witness method can be used to detect a system is over-constrained and computation maximal rigid subsystems leads powerful decomposition method.In first step, we recall theoretical framework solving extend this generate witness. We show then it incrementally over-constrainedness. give an algorithm efficiently identify all parts system. introduce W-decomposition subsystems:...
ABSTRACT Chlamydia felis is an important ocular pathogen in cats worldwide. A multilocus variable-number tandem-repeat analysis (MLVA) system for the detection of tandem repeats across whole genome C. strain Fe/C-56 was developed. Nine selected genetic loci were tested by MLVA 17 isolates, including Baker vaccine strain, and 122 clinical samples from different geographic origins. Analysis results identified 25 distinct patterns. In parallel, a recently described sequence typing scheme...
Current iterative numerical methods, such as continuation or Newton-Raphson, work only on systems for which the corresponding matrix is a square one. The geometric constraint need thus either to have no degrees of freedom, be system software can anchor, i. e. rigid system.