A5002441973- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Yersinia bacterium, plague, ectoparasites research
- Animal Disease Management and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Mosquito-borne diseases and control
- Brucella: diagnosis, epidemiology, treatment
- Diphtheria, Corynebacterium, and Tetanus
- Reproductive tract infections research
- Bacillus and Francisella bacterial research
- Zoonotic diseases and public health
- Insect and Pesticide Research
- Botulinum Toxin and Related Neurological Disorders
- Herpesvirus Infections and Treatments
- Viral Infections and Immunology Research
- Animal Diversity and Health Studies
- Salmonella and Campylobacter epidemiology
- Infective Endocarditis Diagnosis and Management
- Microbial infections and disease research
- Rabies epidemiology and control
- Gut microbiota and health
- Mycobacterium research and diagnosis
- Forensic Entomology and Diptera Studies
- Medical Research and Practices
Ministry of Agriculture, Nature and Food Quality
2021-2023
Wageningen University & Research
2011-2021
Utrecht University
2011-2021
Netherlands Food and Consumer Product Safety Authority
2021
Veterinary Research Institute
2013
Radboud University Nijmegen
2013
Radboud University Medical Center
2013
Schothorst Feed Research
2006
Abstract Q fever is a zoonosis caused by the bacterium Coxiella burnetii. One of largest reported outbreaks in humans occurred Netherlands starting 2007; epidemiologic investigations identified small ruminants as source. To determine genetic background C. burnetii domestic responsible for human outbreak, we genotyped 126 burnetii–positive samples from using 10-loci multilocus variable-number tandem-repeat analyses panel and compared them with internationally known genotypes. unique genotype...
Recently, the number of human Q fever cases in Netherlands increased dramatically. In response to this increase, dairy goats and sheep were vaccinated against Coxiella burnetii. All pregnant herds positive for culled. We identified effect vaccination on bacterial shedding by small ruminants. On day culling, samples uterine fluid, vaginal mucus, milk obtained from 957 animals 13 herds. Prevalence load reduced compared with unvaccinated animals. These effects most pronounced during their first...
Scanning surveillance by the Veterinary Laboratories Agency revealed emergence of suspected botulism in ruminants 2003, presented as flaccid paralysis. From 2003 to 2009, 168 cattle and 19 sheep incidents were recorded, with mortality between 5 80 per cent. All 95 cent had proximity broiler litter. July 2006, gut contents collected from 74 affected 10 tested for Clostridium botulinum toxins using mice bioassays organisms culture. Type D toxin was identified 32 18 samples. C type 40 30...
Background. Current practice for diagnosis of Q fever, caused by the intracellular pathogen Coxiella burnetii, relies mainly on serology and, in prevaccination assessment, skin tests (STs), which both have drawbacks. In this study, C. burnetii–specific interferon γ (IFN-γ) production was used as a new diagnostic tool previous circumventing most these Our aim to compare test and ST. Methods. One thousand five hundred twenty-five individuals from an endemic area with risk chronic fever were...
Between 2006 and 2009 the largest human Q fever epidemic ever described occurred in Netherlands. The source of infection was traced back to dairy goat herds with abortion problems due fever. first aim control measures taken these reduction exposure. To analyze dynamics study effect measures, a within-herd model Coxiella burnetii transmission developed. With this individual-based stochastic we evaluated six strategies three herd management styles studied which strategy leads lower prevalence...
Coxiella burnetii is an obligate intracellular bacterium and the etiological agent of Q fever. During 2007-2010 largest fever outbreak ever reported occurred in The Netherlands. It anticipated that strains from this demonstrated increased zoonotic potential as more than 40,000 individuals were assumed to be infected. acquisition novel genetic factors by these C. strains, such virulence-related genes, has frequently been proposed discussed, but not proved yet. In present study, whole genome...
In poultry several Chlamydia species have been detected, but psittaci and gallinacea appear to be most prevalent important. is a well-known zoonosis considered pathogen of poultry. has described more recently. Its avian pathogenicity zoonotic potential further elucidated. Within the Netherlands no data were available on presence farms. As part surveillance programme for pathogens in farm animals, we investigated pooled faecal samples from 151 randomly selected layer On voluntary base, 69...
Abstract A Brucella suis biovar 1 infection was diagnosed in a dog without typical exposure risks, but the had been fed raw meat–based diet (hare carcasses imported from Argentina). Track and trace investigations revealed that most likely source of dog’s meat diet.
Background. Infection with Coxiella burnetii can lead to acute and chronic Q fever. Toll-like receptor 1 (TLR1), TLR2, TLR4, TLR6, nucleotide-binding oligomerization domain (NOD1), NOD2, the mitogen-activated protein kinases are central in innate immune response against microorganisms, but little is known about their role recognition of C. humans.
Coxiella burnetii is the causative agent of zoonotic disease Q fever. As it an intracellular pathogen, infection by C. requires adaptation to its eukaryotic host and environment. The recently developed cell-free medium also allows bacteria propagate without cells, maintaining potential. different hosts or extracellular environments has been assumed involve genome-wide modulation gene expression. However, little currently known about these events which are critical for understanding survival...
Q fever is caused by the obligate intracellular bacterium Coxiella burnetii. In vitro growth of usually limited to viable eukaryotic host cells imposing experimental constraints for molecular studies, such as identification and characterisation major virulence factors. Studies pathogenicity may benefit from recent development an extracellular medium C. However, it crucial investigate consistency phenotype strains propagated two fundamentally different culturing systems. present study, we...
Bacterial endocarditis is a recognised disease in humans and animals. In humans, infection with Coxiella burnetii can cause endocarditis, but this has not been investigated thoroughly Endocarditis cattle common post-mortem finding abattoirs studies have identified Trueperella pyogenes as major cause. Despite exposure of to C. burnetii, the significance particular bacterium for development progression studied detail. Cardiac valves affected (n = 100) were examined by histology, fluorescence...
For some microbial species, such as Bacillus anthracis, the etiologic agent of disease anthrax, correct detection and identification by molecular methods can be problematic. The virulent B. anthracis is challenging due to multiple virulence markers that need present in order for its close relationship cereus other members group. This especially case environments where build-up spores occur several representatives group may present, which increases chance false-positives. In this study we...
The largest global Q fever outbreak occurred in Netherlands during 2007 to 2010. Goats and sheep were identified as the major sources of disease. Here, we report first complete genome sequence ITALIC! Coxiella burnetiigoat strain NL3262 that an epidemiologically linked chronic human strain, both having outbreak-related CbNL01multilocus variable-number tandem-repeat analysis (MLVA) genotype.
Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the in livestock and human populations. The primary aim of this study was to assess presence Brucella pinnipedialis mammals living along Dutch coast observe possible correlation between B. accompanying pathology found infected animals. overall prevalence spp. antibodies sera from healthy wild grey seals (Halichoerus grypus; n=11) harbor (Phoca vitulina; n=40), collected 2007 2013 ranged...