A5012877592- Veterinary medicine and infectious diseases
- Humic Substances and Bio-Organic Studies
- Microbial infections and disease research
- Animal Behavior and Welfare Studies
- Animal Disease Management and Epidemiology
- Milk Quality and Mastitis in Dairy Cows
- Parasitic Infections and Diagnostics
- Reproductive Physiology in Livestock
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Dermatology and Skin Diseases
- Bioactive Compounds and Antitumor Agents
- Coccidia and coccidiosis research
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Prion Diseases and Protein Misfolding
- Vector-borne infectious diseases
- Clostridium difficile and Clostridium perfringens research
- Vector-Borne Animal Diseases
- Diphtheria, Corynebacterium, and Tetanus
- Mycobacterium research and diagnosis
- Nail Diseases and Treatments
- Animal health and immunology
- Ruminant Nutrition and Digestive Physiology
- Toxoplasma gondii Research Studies
Technical University of Denmark
2014-2024
University of Copenhagen
2013-2024
CS Diagnostics
2020-2021
Danmarks Nationalbank
2017
Swedish Veterinary Agency
2013
Universidade Federal de Minas Gerais
2013
Instytut Historii Nauki im. Ludwika i Aleksandra Birkenmajerów Polskiej Akademii Nauk
2011
Danish Ministry of Food, Agriculture and Fisheries
2004-2007
Norwegian Veterinary Institute
2007
Aalborg University Hospital
2004
ABSTRACT The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total 24 representing variety diets, ages, and herd health status. A library comprising 4,270 cloned rDNA sequences obtained directly PCR 52 samples either ileum, cecum, or colon constructed. In total, 375 phylotypes identified using 97% similarity criterion. Three hundred nine (83%) had <97% to any database...
Bacterial communities in the large intestines of pigs were compared using terminal restriction fragment length polymorphism (T-RFLP) analysis targeting 16S ribosomal DNA. The fed different experimental diets based on either modified standard feed or cooked rice supplemented with dietary fibers. After feeding animals for 2 weeks, differences bacterial community structure spiral colon detected form profiles fragments (T-RFs). Some T-RFs universally distributed, i.e., they found all samples,...
SARS-CoV-2 infection is the cause of COVID-19 in humans. In April 2020, farmed mink (Neovision vision) occurred Netherlands. The first outbreaks Denmark were detected June 2020 three farms. A steep increase number infected farms from September and onwards. Here, we describe prevalence data collected 215 to characterize spread impact disease one third farms, no clinical signs observed. with signs, decreased feed intake, increased mortality respiratory symptoms most frequently observed, during...
ABSTRACT The etiopathogenesis of the skin disease digital dermatitis (DD), an important cause lameness in cattle, remains uncertain. Microscopically, appears to be polymicrobial, with spirochetes as predominant bacteria. objective this study was identify main part bacteria involved DD lesions cattle by using culture-independent molecular methods. Ten different phylotypes Treponema were identified either 16S rRNA gene sequencing from or fluorescence situ hybridization (FISH) analysis...
Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim this study was investigate microbial composition relative number bacteria inflamed intestinal tissue surgically removed from neonates diagnosed with (n=24). populations specimens were characterized by laser capture...
Although treponemes are consistently identified in tissue from bovine digital dermatitis (DD) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. To study microbiomes 27 DD-infected and 10 healthy interdigital skin samples, we used a combination different molecular methods. Deep sequencing 16S rRNA gene variable regions V1-V2 showed that Treponema, Mycoplasma, Fusobacterium Porphyromonas were genera best differentiating DD samples controls....
ABSTRACT The bacteria associated with the infectious claw disease bovine digital dermatitis (DD) are spirochetes of genus Treponema ; however, their environmental reservoir remains unknown. To our knowledge, current study is first report discovery and phylogenetic characterization rRNA gene sequences from DD-associated treponemes in dairy herd environment. Although spread DD appears to be facilitated by wet floors covered slurry, no have been isolated this environment previously....
Metritis and endometritis commonly occur in dairy cows after calving. Although numerous studies have been performed to identify the causative pathogens, a complete overview has not done. Metagenomic analyzed bacterial populations of uterine flush samples from postpartum (pp) cows, but microbiota luminal fluid may differ endometrium itself, important putative pathogens overlooked. In present study, we compared lumen healthy, metritic, endometritic cows. Samples were collected 68 Holstein at...
Abstract Background Abortion is a major source of economic losses in cattle breeding. occurs due to wide range causes, but infections are the most frequently diagnosed. However, establishing an aetiological diagnosis remains challenging large variety bacteria, protozoa, viruses, and fungi that have been associated with abortion cattle. Economic restraints limit diagnostic methods available for routine diagnostics, decomposition conceptus or lack proper fetal and/or maternal samples further...
To investigate which specific bacterial species that were stimulated or inhibited in the proximal colon of pigs when a fructan-rich diet was compared with contained resistant carbohydrates. The study focussed especially on Bifidobacterial by using noncultureable approach.
ABSTRACT Modern pyrosequencing technology allows for a more comprehensive approach than traditional Sanger sequencing elucidating the etiology of bovine digital dermatitis. We sought to describe composition and diversity treponemes in dermatitis lesions by using deep V3 V4 hypervariable regions 16S rRNA gene coupled with species-level taxonomic identification. Treponema -specific PCRs were performed on biopsy specimens originating from 10 different Catalan dairy herds ( n = 36) dermatitis,...
The aim of this study was to assess infectious foot diseases, including identification and characterization Dichelobacter nodosus Treponema spp., in herds having problems with interdigital dermatitis (ID) heel horn erosion (E) control expected have few problems. We also wanted compare diseased healthy cows all herds. included 14 dairy a total 633 cows. Eight had history ID E, 6 were All scored for lameness, diseases on the hind feet recorded after trimming. Swabs biopsies taken from skin 10...
Necrotizing enterocolitis (NEC) in preterm infants develops very rapidly from a mild intolerance to enteral feeding into intestinal mucosal hemorrhage, inflammation, and necrosis. We hypothesized that immediate feeding-induced gut responses precede later clinical NEC symptoms pigs. Fifty-six pigs were fed total parenteral nutrition (TPN) for 48 h followed by 0, 8, 17, or 34 with either colostrum (Colos, n = 20) formula (Form, 31). Macroscopic lesions detected Form throughout the period...
Abstract Background The equine glandular stomach is commonly affected by erosion and ulceration. aim of this study was to assess whether bacteria, including Helicobacter, could be involved in the aetiology gastric lesions seen horses. Results Stomach lesions, as well normal appearing mucosa were obtained from horses slaughtered for human consumption. All samples tested urease activity using Pyloritek ® assay, while mucosal bacterial content evaluated Fluorescence In Situ Hybridisation....
Background: Lawsonia intracellularis causes porcine proliferative enteropathy and is one of the most economically important diseases in modern pig production worldwide.The Enterisol ® Ileitis vaccine have been shown to reduce clinical disease increase weight gain, however, while natural infection with L. can provide complete protection against re-infection, this has not achieved by vaccine.We therefore undertook a detailed characterization immune responses vaccinated pigs (VAC) compared...
Actinobacillus pleuropneumoniae causes pleuropneumonia in pigs, a disease which is associated with high morbidity and mortality, as well impaired animal welfare. To obtain in-depth understanding of this infection, the interplay between virulence factors pathogen defense mechanisms porcine host needs to be elucidated. However, research has traditionally focused on either bacteriology or immunology; an unbiased picture transcriptional responses can obtained by investigating both organisms same...
ABSTRACT Human intestinal spirochetosis, characterized by end-on attachment of densely packed spirochetes to the epithelial surface large intestines as a fringe has been associated with weakly beta-hemolytic Brachyspira aalborgi and ( Serpulina ) pilosicoli . In this study, fluorescent in situ hybridization oligonucleotide probes targeting 16S or 23S rRNA B. aalborgi, , genus was applied 40 sections formalin-fixed, paraffin-embedded biopsy specimens from 23 Danish 15 Norwegian patients...