A5082516474- DNA Repair Mechanisms
- Fungal and yeast genetics research
- Manufacturing Process and Optimization
- VLSI and Analog Circuit Testing
- Yeasts and Rust Fungi Studies
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Genetic Neurodegenerative Diseases
University of Massachusetts Chan Medical School
2011-2021
The fission yeast clade--comprising Schizosaccharomyces pombe, S. octosporus, cryophilus, and japonicus--occupies the basal branch of Ascomycete fungi is an important model eukaryote biology. A comparative annotation these genomes identified a near extinction transposons associated innovation transposon-free centromeres. Expression analysis established that meiotic genes are subject to antisense transcription during vegetative growth, which suggests mechanism for their tight regulation. In...
Loading of the MCM replicative helicase at origins replication is a highly regulated process that precedes DNA in all eukaryotes. The stoichiometry loaded has been proposed to be key determinant when those initiate during S phase. Nevertheless, genome-wide regulation loading and its direct effect on timing remain unclear. In order investigate why some load more than others, we perturbed levels budding yeast cells and, for first time, directly measured same experiment. Reduction through...
Abstract Loading of the MCM replicative helicase onto origins replication is a highly regulated process that precedes DNA in all eukaryotes. The number loaded on has been proposed to be key determinant when those initiate during S phase. Nevertheless, genome-wide characteristics loading and their direct effect timing remain unclear. In order probe dynamics its timing, we perturbed levels budding yeast cells and, for first time, directly measured same experiment. Reduction through degradation...