A5103110502- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Micro and Nano Robotics
- Force Microscopy Techniques and Applications
- 3D Printing in Biomedical Research
- Acoustic Wave Resonator Technologies
- Metal and Thin Film Mechanics
- Microtubule and mitosis dynamics
- GaN-based semiconductor devices and materials
- Genetic and Kidney Cyst Diseases
- Advanced Welding Techniques Analysis
- thermodynamics and calorimetric analyses
- Aluminum Alloys Composites Properties
- Characterization and Applications of Magnetic Nanoparticles
- Cell Image Analysis Techniques
- Protist diversity and phylogeny
- Material Dynamics and Properties
- Metal Forming Simulation Techniques
- NMR spectroscopy and applications
- Diatoms and Algae Research
- Biocrusts and Microbial Ecology
The University of Tokyo
2013-2024
RIKEN Center for Biosystems Dynamics Research
2024
Osaka University
2023
Kyoto University
2017-2019
Meiji University
2016
Tokushima University
2000-2002
In both randomly moving Dictyostelium and mammalian cells, phosphatidylinositol (3,4,5)-trisphosphate F-actin are known to propagate as waves at the membrane act push out protruding edge. To date, however, relationship between wave geometry patterns of amoeboid shape change remains elusive. Here, by using phase map analysis, we show that morphology dynamics discoideum cells can be characterized number, topology, position spatial singularities, i.e., points represent organizing centers...
Multiciliated cells (MCCs) promote fluid flow through coordinated ciliary beating, which requires properly organized basal bodies (BBs). Airway MCCs have large numbers of BBs, are uniformly oriented and, as we show here, align linearly. The mechanism for BB alignment is unexplored. To study this mechanism, developed a long-term and high-resolution live-imaging system used it to observe green fluorescent protein–centrin2–labeled BBs in cultured mouse tracheal MCCs. During MCC differentiation,...
How mechanical stress applied to the actin network modifies turnover has attracted considerable attention. Actomyosin exerts major force on network, which been implicated in stability regulation. However, direct monitoring of immediate changes F-actin alteration actomyosin contraction not achieved. Here we reexamine myosin regulation by using single-molecule speckle analysis actin. To avoid possible errors attributable actin-binding probes, employed DyLight-labeled that distributes identical...
Abstract Animal cells that spread onto a surface often rely on actin‐rich lamellipodial extensions to execute protrusion. Many cell types recently adhered two‐dimensional substrate exhibit protrusion and retraction of their lamellipodia, even though the is not translating. Travelling waves have also been observed, similar those observed in crawling cells. These regular patterns allow quantitative analysis for comparison mathematical models. The periodic fluctuations leading edge position XTC...
Cells change direction of migration by sensing rigidity environment and traction force, yet its underlying mechanism is unclear. Here, we show that tip actin barbed ends serve as an active "force sensor" at the leading edge. We established a method to visualize intracellular single-molecule fluorescent through elastic culture substrate. found immediately after cell edge stretch, assembly increased specifically lamellipodium tip. The rate with increasing stretch speed. Furthermore,...
In parallel with advances in microscale imaging techniques, the fields of biology and materials science have focused on precisely extracting particle properties based their diffusion behavior. Although majority real-world particles exhibit anisotropy, behavior has been studied less than that isotropic particles. this study, we introduce a method for estimating coefficients individual anisotropic using short-trajectory data basis maximum likelihood framework. Traditional estimation techniques...
Although folding reaetions and fluctuations ofsmall single domain proteins have been relatively well understood by now, much less is c[ear for functiona] dynamics oflarger mu]ti-dornain proteins.Here, we present our recent computational work on three case studies of ]arge andlor multi-demain proteins.FiTst, discuss both conformational dynamies adenylate kinase, a mode] allosteric protein, which shows rather rich behaviors.Second, fo]ding-coupled docking in sp]it GFP.Fina]]y, importance...