A5016205222- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Nicotinic Acetylcholine Receptors Study
- Genetic and rare skin diseases.
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Skin and Cellular Biology Research
- Metal-Catalyzed Oxygenation Mechanisms
- Ion channel regulation and function
- Enzyme Production and Characterization
- Advanced biosensing and bioanalysis techniques
- Cellular transport and secretion
- Plant Physiology and Cultivation Studies
- Ubiquitin and proteasome pathways
- Neuroscience and Neuropharmacology Research
- Genetic and Kidney Cyst Diseases
- DNA Repair Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- RNA Interference and Gene Delivery
- Biochemical and Molecular Research
- Viral gastroenteritis research and epidemiology
- Environmental Toxicology and Ecotoxicology
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
Keio University
2013-2024
Okayama University Hospital
2008-2016
Chiba University
2013
Mitsubishi Group (Japan)
1989-2010
Tohoku University
1967-2009
Butte County Office of Education
2000
Cornell University
2000
Valent BioSciences (United States)
2000
Hirosaki University
1998
Sado General Hospital
1994
We previously reported that tau protein kinase I (TPKI) induced normal into a state of paired helical filaments (PHF); this is further confirmed here by immunoblot analysis using several antibodies. also present the amino acid sequence TPKI, which identical to glycogen synthase 3β (GSK3β). Moreover, we found TPKI activity was inseparable from GSK3 throughout purification procedure. These results indicate GSK3β.
Airway epithelial cilia protect the mammalian respiratory system from harmful inhaled materials by providing force necessary for effective mucociliary clearance. Ciliary beating is asymmetric, composed of clearly distinguished and recovery strokes. Neither importance nor essential components responsible asymmetry has been directly elucidated. We report here that crucial ciliary function requires tubulin glutamylation, a unique posttranslational modification highly abundant in cilia. WT...
A sequence-specific endonuclease, Taq I, of novel specificity has been partially purified from an extreme thermophile, Thermus aquaticus. The enzyme cleaves bacteriophage lambda DNA at many (greater than 30) sites and psiX174 RF 10 sites. is active temperatures up to 70 degrees. cleavage on have mapped. sequence recognized cleaved by I shown be the symmetrical tetranucleotide: (formula: see text).
Patients with disseminated superficial actinic porokeratosis (DSAP) and linear (LP) exhibit monoallelic germline mutations in genes encoding mevalonate pathway enzymes, such as MVD or MVK. Here, we showed that each skin lesion of DSAP exhibited an individual second hit genetic change the wild-type allele corresponding gene specifically epidermis, indicating a postnatal triggering biallelic deficiency is required for to develop. Most lesions one two principal hits, either somatic homologous...
These peptides inhibit the contractile response of directly stimulated mouse diaphragm.
Superoxide dismutase has been isolated and characterised from the extreme thermophile Thermus aquaticus. The pure enzyme is a reddish-purple manganese-containing protein with molecular weight of approximately 80000 +/- 5000. Combination gel electrophoresis in dodecylsulphate amino acid analysis shows that it composed four identical subunit polypeptide chains consisting 186 acids. tetrameric contains two atoms manganese. A stable manganese-free apoprotein prepared by treatment EDTA 8 M urea...
A novel brain‐specific 25 kDa protein (p25) was purified from a bovine brain extract. The phosphorylated by Ser/Thr‐Pro kinase (TPK II) in tau fractions at the Ser residues of Ser‐Pro sequences. Using immunoblot analysis, found only extracts, and most abundant regions such as cerebrum hippocampus, but less cerebellum, medulla oblongata olfactory bulb. detected rat, human indicating that this specifically exists mammalian tissues.
1. Erabutoxin b, a neurotoxic protein produced by sea snake, Laticauda semifasciata, was iodinated at pH 9.6. A toxic derivative containing two iodine atoms per mole, isolated from the reaction products CM-cellulose column chromatography. 2. The diiodo-erabutoxin b is chromatographically homogeneous, and crystallizable. amino acid analysis of preparation showed loss one histidyl residues original toxin. Diiodohistidine detected in pronase digest toxin derivative. a, which differs erabutoxin...
The reproductive cycle and shell microgrowth patterns of the venerid bivalve Ruditapes philippinarum from Matsukawa-ura, a small inlet facing Pacific Ocean, northeastern Japan were examined. Histological examination gonads revealed that spawning in this species occurred twice 2005, once between late June early August other September October. Comparison with developmental stages gonad each individual individuals (mature stages), mean lunar-day growth rates significantly smaller than those...
Manganese-containing superoxide dismutase was isolated from an extreme thermophile, Thermus thermophilus HB8. About 150 mg of the enzyme obtained 500 g wet cells. The easily crystallized in octahedra ammonium sulfate solution. molecular weight determined to be 8.2 X 10(4) and 8.4 by sedimentation equilibrium gel-filtration, respectively. contains 2 atoms manganese per mole consists four subunits identical weight, about 2.1 10(4). amino acid composition is similar that aquaticus. Proline...
A DNA methylase was purified in a homogeneous state from extremely thermophilic bacterium, Thermus thermophilus HB8, by chromatography on, successively, phosphocellulose, CM-cellulose, and heparin-Sepharose. The molecular weight of the enzyme determined to be about 44,000 gel filtration on Sephadex G-100 column 41,000 SDS-poly-acrylamide electrophoresis, these findings suggest single polypeptide enzyme. develops maximum activity around pH 7.4 at 70 degrees C. Enzymatic is completely...
A second site specific endonuclease with novel specificity has been purified from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at temperature up to 80 degrees C requires Mg2+ or Mn2+ for activity. Tth111II cleaves phi X174RFDNA into 11 fragments lambda NA more than 25 fragments. From the 5'-terminal sequences of TthlllII determined by two dimensional homochromatography survey on nucleotide sequence X174RFDNA, it was concluded that recognizes DNA (see former index)...
1. RNAase (ribonuclease) U2, a purine-specific RNAase, was reduced, aminoethylated and hydrolysed with trypsin, chymotrypsin thermolysin. On the basis of analyses resulting peptides, complete amino acid sequence U2 determined, 2. When compared T1 (EC 3.1.4.8), following regions were found to be similar in two enzymes; Tyr-Pro-His-Gln-Tyr (38-42) Tyr-Pro-His-Lys-Tyr T1, Glu-Phe-Pro-Leu-Val (61-65) Glu-Trp-Pro-Ile-Leu (58-62) Asp-Arg-Val-Ile-Tyr-Gln (83-88) Asp-Arg-Val-Phe-Asn (76-81)...
Abstract The two neurotoxic proteins from the venom of sea snake Laticauda semifasciata erabutoxin a and b form isomorphous orthorhombic crystals: space group P212121 with = 50.0 ± 0.1 A, 46.5 c 21.2 A (erabutoxin a) 49.76 0.05 46.77 0.04 21.58 0.02 b). Erabutoxin has been modified by iodination histidine 26 residue derivative crystallized in 51.5 0.5 47.4 21.8 A. helical potential amino acid sequence studied.
A site specific endonuclease with novel specificity has been isolated from Thermus thermophilus strain 111 and named Tth111I. Tth111I cleaves λ DNA into three fragments of 23.5, 25.7 50.8 % the total length, ColE1 two nearly equal length. The sequences around cleavage sites were determined by Maxam Gilbert method dimensional mapping method. results suggest that recognizes sequence ↓5'-TGACNNNGTC-3′3′-ACTGNNNCAG-5′↑ as indicated arrows. Assuming first T. pair in can be replaced for any base...
Some ubiquitin-like (UBL) domain-containing proteins are known to play roles in receptor trafficking. Alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs) undergo constitutive cycling between the intracellular compartment and cell surface central nervous system. However, function of UBL recycling AMPARs synaptic has not yet been reported.Here, we report that Transmembrane 1 (Tmub1) protein, formerly as Hepatocyte Odd Protein Shuttling (HOPS) which is abundantly...