A5039216548- Wound Healing and Treatments
- melanin and skin pigmentation
- Skin Protection and Aging
- Skin and Cellular Biology Research
- Silk-based biomaterials and applications
- Burn Injury Management and Outcomes
- Corneal Surgery and Treatments
- RNA regulation and disease
- Mesenchymal stem cell research
- Polyamine Metabolism and Applications
- Mast cells and histamine
- Ocular Surface and Contact Lens
- Tissue Engineering and Regenerative Medicine
- Plant Reproductive Biology
- Cellular Mechanics and Interactions
- Atherosclerosis and Cardiovascular Diseases
- Corneal surgery and disorders
- Biomedical Ethics and Regulation
- Planarian Biology and Electrostimulation
- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- Mathematical Biology Tumor Growth
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Oral and gingival health research
J-Power (Japan)
2014
Toyama College
1999
Lead Chemical (Japan)
1998
Tohoku Medical and Pharmaceutical University
1987
In Japan, the cultured epithelial autografts "JACE" was accepted as a health insurance adaptation from January 1, 2009. We examined extensive burn wounds in 8 patients by using combination of autograft and JACE. After debridement, we managed wound bed preparation artificial dermis. The covered with fine tissue 2 weeks after implanted dermis trafermin used every day. Meshed 6:1 split-thickness were placed onto recipient under epidermalization nearly complete within 3 to 4 weeks.A total 39...
No efficient treatment has yet been established for epidermolytic ichthyosis (EI), which is caused by pathogenic variants of KRT1 or KRT10. Patients with confetti (IWC) have multiple normal-appearing spots, the revertant somatic recombination that occurs at each spot independently. Additionally, some patients EI large areas normal skin due to postzygotic mosaicism.
We have reported that high-hydrostatic-pressure (HHP) technology is safe and useful for producing various kinds of decellularized tissue. However, the preparation or inactivated skin using HHP has not been reported. The objective this study was thus to prepare from human HHP, explore appropriate conditions pressurization inactivate can be used reconstruction. Human samples 8 mm in diameter were packed bags filled with normal saline solution (NSS) distilled water (DW), then pressurized at 0,...
DNA methylation is considered the primary epigenetic mechanism underlying development of malignant melanoma. Since can be influenced by environmental factors, it preferable to compare cancer and normal cells from same patient. In order status in melanoma tissues melanocytes individuals, we employed a novel epidermal sheet cultivation technique isolate unaffected sites patients. We also analyzed metastatic samples, three commercially available melanocytes, four cell lines. Cluster analysis...
Giant congenital melanocytic nevi are intractable lesions associated with a risk of melanoma. High hydrostatic pressure (HHP) technology is safe physical method for producing decellularized tissues without chemicals. We have reported that HHP can inactivate cells present in various damaging the native extracellular matrix (ECM). The objectives this study were to human nevus tissue using and explore possibility reconstructing skin inactivated combination cultured epidermis (CE). Human...
Hog gastric vesicles showed Cl- conductance when treated with Cu2+-o-phenanthroline, an S-S cross-linking reagent. An IgG monoclonal antibody caused dose-dependent inhibition of that had been induced by cross-linking. The did not cause intervesicular aggregation, as determined measurement vesicle size. These results show conductance, the stimulation and which are regulated reversibly S-S—2SH transformation, is due to native, physiological channels. also dose dependently inhibited activities...
As the take rate of cultured epidermal autografts in burn wound treatment is variable, widely expanded meshed auto skin grafts are often used combination with autograft to increase and achieve definitive coverage. However, a long time (3-4 weeks) required prepare epidermis sheet disadvantage. Allogeneic can be prepared advance cryopreserved for treating third-degree burns. Nevertheless, human (hCE) has not been proved accelerate healing after grafting. Here, we investigated effect hCE on rat...
Abstract Corneal limbal epithelial stem cell transplantation using cultivated human corneal sheets has been used successfully to treat deficiencies. Here we report an investigation into the quality of time-lapse imaging culture process every 20 minutes over 14 days ascertain level jamming, a phenomenon in which cells become smaller, more rounded and less actively expansive. In parallel, also assessed expression p63, important marker. The occurrence jamming was variable transient, but...
We previously reported that human nevus tissue was inactivated after high hydrostatic pressure (HHP) higher than 200 MPa and cultured epidermis (hCE) engrafted on the pressurized at but not 1000 MPa. In this study, we explore changes to epidermal basement membrane in detail elucidate cause of difference hCE engraftment. Nevus specimens 8 mm diameter were divided into five groups (control 100, 200, 500, MPa). Immediately HHP, immunohistochemical staining performed detect presence laminin-332...
Abstract Background Acquired skin hypopigmentation has many etiologies, including autoimmune melanocyte destruction, aging, inflammation, and chemical exposure. Distinguishing lesions from normally pigmented is clinically important to precisely assess disease severity. However, no gold standard assessment method been reported. We aimed investigate whether spectrophotometers are useful for assessing vitiligo rhododendrol (4‐(4‐hydroxyphenol)‐2‐butanol) (Rhododenol ® )‐induced leukoderma...
Cryopreserved allogeneic cultured epidermis (CE) is used for treating second-degree burn wounds and diabetic foot ulcers; however, the need cryopreservation limits its use. We have previously reported that CE accelerates wound healing irrespective of viability hypothesized dehydrated CEs lacking living cells may act as an effective dressing. prepared dried investigated morphological physical properties wound-healing effects compared them with those cryopreserved CE. Hematoxylin-eosin...
Autologous cultured epidermis (CE) is an effective approach for overcoming the deficiency of donor sites to treat extensive burns. However, production autologous CE takes 3–4 weeks, which prevents its use during life-threatening period severe In contrast, allogeneic can be prepared in advance and used as a wound dressing, releasing several growth factors stimulating activity recipient cells at application site. Dried by drying CEs under controlled temperature humidity conditions until all...
Abstract Cultured epidermal autografts (CEA) are surgical therapeutic alternatives for patients with stable vitiligo resistant to conventional medical treatments. In the present study, we assessed color matching before and at 12 months after CEA treatment. Eleven 16 lesions were included in this prospective study. The recipient sites prepared by CO 2 laser superficial ablation subjected application. We clinically evaluated categorized of repigmented skin as well percentage repigmentation....
Cultured epidermal autografting has been employed in a variety of clinical treatments including vitiligo management. In this study, we successfully treated 2 patients with using short-pulsed CO2 laser and by grafting the autologous cultured epidermis. Small pieces uninvolved skin (2 x 1 cm) were taken for cultivation from pudendal or axillary area expanded into sheets 100 cm. Before grafting, lesions abraded superficially computerized pattern generator. After successful repigmentation was...
Allogeneic cultured epidermis (allo-CE) is a keratinocyte sheet manufactured from donor cells and promotes wound healing when used in deep dermal burns, sites, chronic ulcers serves as dressing. Allo-CE usually cryopreserved to be ready use. However, the cryopreservation procedure will damage cell viability, influence of Allo-CE, according its viability or process, has not been evaluated sufficiently. In this study, we aimed prove contained allo-CEs on healing. We prepared CEs with Green’s...