A5101653294- Urinary Bladder and Prostate Research
- Pelvic floor disorders treatments
- Bladder and Urothelial Cancer Treatments
- Bacterial Genetics and Biotechnology
- Renal cell carcinoma treatment
- Antibiotic Resistance in Bacteria
- Prostate Cancer Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Cancer Immunotherapy and Biomarkers
- Urinary Tract Infections Management
- Escherichia coli research studies
- Urinary and Genital Oncology Studies
- Cancer therapeutics and mechanisms
- Hormonal and reproductive studies
- Urological Disorders and Treatments
- Gastrointestinal disorders and treatments
- Gastrointestinal Tumor Research and Treatment
- Lipoproteins and Cardiovascular Health
- Renal and related cancers
- Metastasis and carcinoma case studies
- Neuroendocrine Tumor Research Advances
- Peptidase Inhibition and Analysis
- Glioma Diagnosis and Treatment
- Urologic and reproductive health conditions
- Genetic factors in colorectal cancer
Aichi Medical University
2015-2025
Yamaguchi University Hospital
2024
Yamaguchi University
2007-2024
University of Occupational and Environmental Health Japan
1999-2024
Toyota Memorial Hospital
2023
University of Fukui
2013-2022
University of Fukui Hospital
2022
Obayashi (Japan)
2021
Weatherford College
2021
Southern Illinois University School of Medicine
2019
Autophagy is originally named as a process of protein recycling. It begins with sequestering cytoplasmic organelles in membrane vacuole called autophagosome. Autophagosomes then fuse lysosomes, where the materials inside are degraded and recycled. To date, however, little known about role autophagy cancer therapy. In this study, we present that temozolomide (TMZ), new alkylating agent, inhibited viability malignant glioma cells dose-dependent manner induced G2/M arrest. At clinically...
The authors examined 50 patients with cerebral glioma use of positron emission tomography (PET) and L-[methyl]-[11C]methionine to assess the grade malignancy extent glioma. Carbon-11 methionine was highly accumulated in lesion 31 32 high-grade 11 18 low-grade rate uptake C-11 significantly higher than (P < .001). However, individual cases it difficult evaluate only from degree accumulation. In most cases, area increased accumulation did not correspond abnormalities seen at computed (CT)....
We have previously found that a pyrazole derivative 1 possesses antibacterial activity and inhibitory against DNA gyrase topoisomerase IV. Here, we synthesized new derivatives 5-[(E)-2-(5-chloroindol-3-yl)vinyl]pyrazole 16 potent selective bacterial topoisomerases. Many of the were clinically isolated quinolone- or coumarin-resistant Gram-positive strains had minimal concentration values these equivalent to those susceptible strains.
Autophagy is a novel response of cancer cells to ionizing radiation (IR) or chemotherapy, but its significance mechanism remains largely elusive. characterized with the prominent formation autophagic vacuoles in cytoplasm. It protein degradation system that involves autophagic/lysosomal compartment. The process begins sequestering portion cytoplasm, forming autophagosome. autophagosome then fuses lysosome and lyses contents. To study radiation-induced autophagy specific molecules, we...
Abstract —When adherent cells, such as epithelial or endothelial are detached and continuously maintained in suspension, they undergo a form of programmed cell death termed anoikis. We demonstrate that coincident with detachment, there is dramatic rise the intracellular level reactive oxygen species (ROS). Reattachment to solid surface rapidly attenuates ROS. The mitochondria appear be major source detachment-induced change redox state appears contribute anoikis, because treatment either...
Conditionally replicating adenoviruses (CRAds) can be engineered to replicate selectively in cancer cells and cause cancer-specific cell lysis; thus they are considered a promising therapy.To elucidate the mechanisms by which CRAds induce death, we infected normal human fibroblasts (MRC5, telomerase negative), malignant glioma (U373-MG U87-MG), cervical (HeLa), prostate (PC3) (all positive) with regulated reverse transcriptase promoter (hTERT-Ad) or control nonreplicating (Ad-GFP)....
Telomerase is a ribonucleoprotein enzyme which stabilizes chromosomal structure, thereby inducing cellular immortality. Three major subunits composing telomerase complex have been cloned, designated hTR (human RNA), TPI (telomerase-associated protein I), and hTERT reverse transcriptase). In the present study, total of 36 renal-cell carcinomas (RCC) adjacent normal tissues, as well cell lines derived from RCC or kidney, were examined for expression each subunit activity. RT-PCR analyses...
Abstract BACKGROUND Expression profiles of some microRNAs (miRNAs) were associated with clinicopathological findings in human prostate cancer (PC), but the relative expression miRNAs among Gleason patterns (GPs) remains unclear. In this study, we investigated several known each GP PC. METHODS Formalin‐fixed, paraffin embedded (FFPE) tissue samples obtained from radical prostatectomy (RP) (patient set 1, n = 43, including (GP 3) 22, 4) 35, and 5) 12) needle biopsy 2, 10, 10). Cancer tissues...
// Shingo Inaguma 1 , Miho Riku Hideaki Ito Takumi Tsunoda Hiroshi Ikeda Kenji Kasai Department of Pathology, Aichi Medical University School Medicine, Nagakute, 480-1195, Japan Correspondence to: Kasai, e-mail: kkasai@aichi-med-u.ac.jp Keywords: GLI1, CXCR4, CXCR7, migration, breast cancer Received: July 30, 2015 Accepted: September 04, Published: 16, 2015 ABSTRACT The up-regulation chemokine receptors CXCR4 and CXCR7 impacts on the distant metastasis prognosis...
Despite the confirmed anti-cancer effects of T-cell immune checkpoint inhibitors, in colorectal cancer (CRC) they are only effective a small subset patients with microsatellite-unstable tumors. Thus, therapeutics targeting other types CRCs or tumors refractory to inhibitors desired. The binding aberrantly expressed CD47 on tumor cells signal regulatory protein-alpha (SIRPA) macrophages allows evade destruction. Based these observations, drugs macrophage have been developed expectation...
Abstract Immunotherapy with immune‐checkpoint therapy has recently been used to treat oral squamous cell carcinomas (OSCCs). However, improvements in current immunotherapy are expected because response rates limited. Transforming growth factor‐β (TGF‐β) creates an immunosuppressive tumor microenvironment (TME) by inducing the production of regulatory T‐cells (Tregs) and cancer‐associated fibroblasts inhibiting function cytotoxic T‐lymphocytes (CTLs) natural killer cells. TGF‐β may be...
Dendritic spine formation/maintenance is highly dependent on actin cytoskeletal dynamics, which regulated by small GTPases Rac1 and Cdc42 through their downstream p21-activated kinase/LIM-kinase-I/cofilin pathway. ARHGEF7, also known as ß-PIX, a guanine nucleotide exchange factor for Cdc42, thereby activating Rac1/Cdc42 the pathway, leading to upregulation of formation/maintenance. We found that STIL, one primary microcephaly gene products, associated with ARHGEF7 in dendritic spines...
Abstract To identify the type of Verotoxins (VT) produced by Verocytotoxin‐producing Escherichia coli (VTEC), a sensitive bead‐enzyme‐linked immunosorbent assay and polymerase chain reaction with common specific primers to various VTs (VT1, VT2, VT2vha, VT2vhb, VT2vp1) were developed. Together colony hybridization tests oligo‐ polynucleotide probes, these methods applied VTEC isolates VT produced. The toxin types 26 37 strains identified, but profiles in assays remaining 11 suggested...
Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)‑linked cell surface protein that highly expressed in several types of malignant tumor, including pleural mesothelioma, ovarian cancer and pancreatic adenocarcinoma. Recently, comprehensive immunohistochemical study using MN‑1 monoclonal antibody identified significant number colorectal tumors which MSLN was expressed. However, the clinicopathological profiles survival patients with MSLN‑positive have not been fully analyzed. In current...
DNA repair enzymes damaged by platinum agents and ionising radiation. Single nucleotide polymorphisms (SNPs) in genes modulate the capacity might affect response prognosis following platinum-based chemoradiotherapy (CRT). We investigated associations between functional SNPs survival muscle-invasive bladder cancer patients treated with CRT to determine predictive value of patient selection for conservation therapy. The study group comprised 78 who underwent transitional cell carcinoma...
Coumarin-resistant mutants of Staphylococcus aureus were isolated by three-step selection with novobiocin at different concentrations. Sequencing analysis the gyrB and parE genes first-, second-, third-step revealed that successive point mutations first occurred specifically in gene, followed a mutation gene then an additional gene. These findings demonstrate DNA gyrase is primary target topoisomerase IV secondary for accumulation both associated high-level resistance to S. aureus. Moreover,...
Acriflavine resistance in the clinical meticillin-resistant Staphylococcus aureus isolate KT24 was found not to be mediated by multidrug efflux pumps encoded qacA/B, smr, qacE, qacG, qacH, qacJ or norA. Early uptake and accumulation of ethidium bromide MRSA significantly lower than that a susceptible strain, although rates were similar. Therefore, permeability barrier may conceivable mechanism acriflavine resistance. Interestingly, it had thickened cell wall, cell-wall thickness increased...