A5102815898- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Polyamine Metabolism and Applications
- Protein Structure and Dynamics
- Amino Acid Enzymes and Metabolism
- Biochemical and Molecular Research
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- DNA and Nucleic Acid Chemistry
- Hemoglobin structure and function
- Metabolism and Genetic Disorders
- Metalloenzymes and iron-sulfur proteins
- ATP Synthase and ATPases Research
- Photoreceptor and optogenetics research
- Origins and Evolution of Life
- Microbial Community Ecology and Physiology
- Enzyme Production and Characterization
- Biopolymer Synthesis and Applications
- Bacterial Genetics and Biotechnology
- Space Science and Extraterrestrial Life
Kyowa-kako (Japan)
2013-2023
Chiba Institute of Science
2023
ENVIRON (United States)
2015
Kyushu University
2012
Tokyo University of Pharmacy and Life Sciences
2001-2011
Chiba Institute of Technology
2009
Institute of Microbiology
2005-2007
Tokyo Institute of Technology
1992-2005
The University of Tokyo
1975-2003
Kyoto University Institute for Chemical Research
1997
Active transport across the vacuolar components of eukaryotic endomembrane system is energized by a specific H+-ATPase. The amino acid sequences 70- and 60-kDa subunits H+-ATPase are approximately equal to 25% identical beta alpha subunits, respectively, eubacterial-type F0F1-ATPases. We now report that same 50% sulfur-metabolizing Sulfolobus acidocaldarius, an archaebacterium (Archaeobacterium). Moreover, homologue 88-amino stretch near amino-terminal end 70-kDa subunit absent from...
The properties of an extremely thermophilic bacterium isolated from water at a Japanese hot spring and previously named Flavobacterium thermophilum are described. cells gram-negative, nonsporulating, aerobic rods containing yellow pigment. optimum temperature for growth is between 65 72 C, the maximum being 85 C minimum 47 C. guanine plus cytosine content deoxyribonucleic acid thermophile 69 mol %. This microorganism sensitive to various antibiotics including those which known be rather...
Emerging X-ray free-electron lasers with femtosecond pulse duration enable single-shot snapshot imaging almost free from sample damage by outrunning major radiation processes. In bioimaging, it is essential to keep the close its natural state. Conventional high-resolution imaging, however, suffers severe that hinders live cell imaging. Here we present a method for capturing snapshots of cells kept in micro-liquid enclosure array laser diffraction. We place living Microbacterium lacticum an...
In studies on the cause of extreme stability macromolecules Thermus thermophilwr HB8, leuB gene coding for 3-isopropylmalate dehydrogenase leucine synthesis pathway and its flanking regions were cloned sequenced.The T. thermophilus was expressed in a leuB-less mutant Escherichia coli, thermostable purified from an extract cells.The primary structure thermophilic isopropylmalate deduced nucleotide sequence (1017 base pairs) amino acid peptides isolated dehydrogenase.The has M, = 35,968, value...
Knots in polypeptide chains have been found very few proteins. Only two proteins are considered to a shallow `trefoil' knot, which tucks residues at one end of the chain through loop exposed on protein surface. Recently, another was by mathematical algorithm deep `figure-of-eight' knot had not visually identified. In present study, crystal structure hypothetical RNA 2′-O-ribose methyltransferase from Thermus thermophilus (RrmA) determined 2.4 Å resolution and trefoil for first time. The is...
The gene of an extreme thermophile, Thermus thermophilus HB8, which codes for a leucine biosynthetic enzyme, 3-isopropylmalate (3-IPM) dehydrogenase [EC 1.1.1.85], was cloned in Escherichia coli using pBR322 as vector. E. cells carrying this recombinant plasmid, pHB2, produced the thermophilic enzyme 7-fold more than did T. HB8 cells. When crude extract pHB2-carrying treated at 70 degrees C 10 min, approximately 75% protein precipitated with full activity 3-IPM being left supernatant,...
We cloned and sequenced the leuB gene encoding 3‐isopropylmalate dehydrogenase from Escherichia coli K‐12 (JM103). Errors (33 residues) were found corrected in sequence previously reported for of Thermus thermophilus . The three‐dimensional structure thermophile enzyme amino acid comparison suggested that a part high stability T. is conferred by increased hydrophobic interaction at subunit‐subunit interface. Two residues interface enzyme, Leu246 Val249, are substituted with less residues,...
Extreme thermophiles produce two types of unusual polyamine: long linear polyamines such as caldopentamine and caldohexamine, branched quaternary ammonium compounds [e.g. tetrakis(3-aminopropyl)ammonium]. To clarify the physiological roles in thermophiles, we synthesized them chemically tested their effects on stability ds (double-stranded) ss (single-stranded) DNAs tRNA response to thermal denaturation, measured by differential scanning calorimetry. Linear stabilized dsDNA proportion number...
Isolation of novel membrane-associated ATPases, presumably soluble parts the H+-ATPases, from archaebacteria has been recently reported, and their properties were found to be significantly different usual F1-ATPase. In order assess relationship archaebacterial ATPases F1-ATPases other known amino acid sequence alpha subunit ATPase Sulfolobus acidocaldarius, an acidothermophilic archaebacterium, was compared with sequences ATPases. The gene encoding its cloned genomic library S. nucleotide...
A bacteriophage (phiYS40) infectious to an extreme thermophile, Thermus thermophilus HB8, was isolated and characterized. phiYS40 grows over the temperature range of 56 78 C, optimum growth is about 65 C. The phage had a latent period 80 min burst size at has hexagonal head 0.125 mum in diameter, tail 0.178 long 0.027 wide, base plate fibers. thermostable broth but rather unstable buffer containing 10 mM Tris, MgCl2, pH 7.5. addition Casamino Acids (1 percent), polypeptone (0.8 yeast extract...
Thermoplasma acidophilum HO-62 was grown at different pHs and temperatures, its polar lipid compositions were determined. Although the number of cyclopentane rings in caldarchaeol moiety increased when T. cultured high temperature, decreased low pHs. Glycolipids, phosphoglycolipids, phospholipids analyzed by high-performance liquid chromatography with an evaporative light-scattering detector. The amount more than two sugar units on one side under low-pH high-temperature conditions. amounts...
An extreme thermophile has been isolated from a Japanese thermal spa.To elucidate the molecular basis of thermophily, chemical and physical natures cell constituents microorganism are under investigation.In this communication bacterial properties heat stability t-RNA ribosomes will be described.Aliquots (about 2 ml) water sampled hot spring at Mine Hot Spring, Shizuoka, ,Japan, were transferred into polypeptone-yeast extract-sodium chloride media (final 8, 4, 3%) pH 7.0, incubated 75°.The...
The complete nucleotide sequences of the mitochondrial (mt) genomes three cephalopods, Octopus vulgaris (Octopodiformes, Octopoda, Incirrata), Todarodes pacificus (Decapodiformes, Oegopsida, Ommastrephidae), and Watasenia scintillans Enoploteuthidae), were determined. These mt encode standard set metazoan genes. However, W. T. share duplications longest noncoding region, cytochrome oxidase subunit genes two ATP synthase genes, tRNA(Asp) gene. Southern hybridization analysis genome shows that...
The gene which encodes the beta subunit of novel membrane-associated ATPase has been identified and characterized. subunit, is most likely soluble part non-F0F1 type H+-ATPase, was obtained from archaebacterium, Sulfolobus acidocaldarius. In terms its location, it follows just after for alpha subunit. It comprised 1398 nucleotides, corresponding to a protein 465 amino acids, consensus sequence in nucleotide binding proteins poorly conserved. Together with previously described results,...
An ATPase with Mr of 360,000 was purified from plasma membranes a thermophilic eubacterium Thermus thermophilus, and characterized. ATP hydrolytic activity the enzyme extremely low, 0.07 mumol Pi released mg-1 min-1, it stimulated up to 30-fold by bisulfite. The following properties indicate that is not usual F1-ATPase but belongs V-type family, another class ATPases found in archaebacteria eukaryotic endomembranes. Among its four kinds subunits approximate values 66,000 (alpha), 55,000...
The catalytic and allosteric sites of proton translocating adenosine triphosphatase (ATPase) were studied by measuring the binding nucleotides to ATPase, its α β subunits purified from thermophilic bacterium PS3, with a circular dichroic spectrometer. In contrast mesophilic ATPases, this enzyme contained no tightly bound nucleotides, stable after their purification. These properties advantageous for analyzing both sites. former site showed rapid loose binding, but latter slow (t1/2≒1 h, ADP)...
Abstract A new polyamine has been extracted from an extreme thermophile, Thermus thermophilus, and its chemical structure was determined as 1,12-diamino-4,8-diazadodecane, NH2(CH2)3NH(CH2)3NH(CH2)4NH2, based on proton NMR, 13C mass spectra. trivial name thermospermine is proposed for the compound.