A5034298957- Amino Acid Enzymes and Metabolism
- Biochemical and Molecular Research
- Biopolymer Synthesis and Applications
- Carbohydrate Chemistry and Synthesis
- Cancer Research and Treatments
- DNA and Nucleic Acid Chemistry
- Fungal Biology and Applications
- Chemical Synthesis and Analysis
- Neuropeptides and Animal Physiology
- Adenosine and Purinergic Signaling
- Advanced biosensing and bioanalysis techniques
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- Hearing Loss and Rehabilitation
- Neuroscience and Neuropharmacology Research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Polyamine Metabolism and Applications
- Acute Myeloid Leukemia Research
- Herpesvirus Infections and Treatments
- Glycosylation and Glycoproteins Research
- Acute Lymphoblastic Leukemia research
- Chemical Synthesis and Reactions
- T-cell and B-cell Immunology
- Energy, Environment, Agriculture Analysis
Kyorin University
1997-2023
Tohoku University
2000-2018
Tokyo University of Technology
2014-2015
Liberal Arts University
1992-2014
Nagasaki University
2013
Ishikawa Prefectural Central Hospital
2006
Eisai (Japan)
1978-2002
Central Institute for Experimental Animals
2001
Nihon University
2001
The University of Tokyo
1977-2001
L-Lysine a-oxidase from Trichoderma vi& Y244-2 has been purified to homogeneity.The enzyme shows absorption maxima at 277, 388, and 466 nm a shoulder around 490 n m contains 2 mol of FAD/ enzyme.The molecular weight approximately 116,000 consists two subunits identical in (about 56,000).In addition L-lysine, L-ornithine, L-phenylalanine, L-tyrosine, L- arginine, L-histidine are oxidized by the lesser extent.Several lysine analogs such as &hydroxylysine efficiently.Balance studies showed that...
[reaction--see text] Indoles are selectively acylated at the 3-position in high yields on treatment with a wide variety of acyl chlorides CH(2)Cl(2) presence diethylaluminum chloride or dimethylaluminum chloride. The reaction proceeds under mild conditions and is applicable to indoles bearing various functional groups without NH protection.
1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl) uracil (BV-ara-U) and 1-beta-D-arabinofuranosyl-E-5-(2-chlorovinyl)uracil (CV-ara-U) were tested for their anti-herpesviral activity in virus rating method, a plaque reduction yield using human embryonic lung fibroblast (HEL-F) cells, At concentration as low 0.1 microgram/ml, both drugs exerted marked inhibitory effect on the development of cytopathogenic induced by herpes simplex type 1 (HSV-1) infection multiplication formation HSV-1. Neither...
A new flavoprotein enzyme, L-glutamate oxidase, was purified to homogeneity from an aqueous extract of a wheat bran culture Streptomyces sp. X-119-6. It showed absorption maxima at 273, 385 and 465nm shoulder around 490nm, contained 2 mol FAD per enzyme. The enzyme had molecular weight approximately 140, 000 consisted three sizes subunits with weights 44, 000, 16, 9, 000. Balance studies that 1 converted α-ketoglutarate, ammonia hydrogen peroxide the consumption oxygen. In addition...
A new flavoprotein enzyme, l-glutamate oxidase, was purified to homogeneity from an aqueous extract of a wheat bran culture Streptomyces sp. X-l 19–6. It showed absorption maxima at 273, 385 and 465 nm shoulder around 490 nm, contained 2 mol FAD per enzyme. The enzyme had molecular weight approximately 140,000 consisted three sizes subunits with weights 44,000, 16,000 9,000. Balance studies that 1 converted α-ketoglutarate, ammonia hydrogen peroxide the consumption oxygen. In addition...
Thymidine analogs highly active against herpes simplex virus were compared in their inhibitory action seven strains of varicella-zoster by a plaque reduction assay. E-5-Bromovinyl-arabinosyluracil (BV-ara-U) was most active, followed E-5 chlorovinyl-arabinosyluracil, E-5-bromovinyl-2'-deoxyuridine (BV-dUrd), 2'-fluoro-5-methyl-arabinosyluracil, 2'-fluoro-5-iodo-arabinosylcytosine, arabinosylthymine, 5-vinyl-arabinosyluracil, acycloguanosine, and 5-iodo-2'-deoxyuridine, order to decreasing...
Immunophenotyping was performed in 1044 consecutive childhood acute lymphoblastic leukemia (ALL) patients enrolled the Tokyo Children's Cancer Study Group L04-16 trial, revealing novel findings associated with genetic abnormalities. In addition to TCF3-PBX1 and MEF2D fusions, CD10(+) subtype of KMT2A-MLLT3-positive ALL frequently exhibited cytoplasmic-μ(+) pre-B immunophenotype. Although ETV6-RUNX1 significantly correlated myeloid antigen expression, more than half expressed neither CD33 nor...
Although umbilical cord blood (CB) is increasingly being used as an alternative to bone marrow (BM) a source of transplantable hematopoietic stem cells (HSC), information on the repopulating ability CB HSC still limited. We recently established xenotransplantation system in NOD/Shi-scid mice evaluate human cell activity. In present study, we transplanted 5 10 x 10(4) CD34(+) into six treated with anti-asialo GM1 antiserum investigate CB. The BM all recipients contained CD45(+) 12 weeks after...
Abstract Treatment of various alcohols with 1-methyl-2-fluoropyridinium salt and sodium N,N-dimethyldithiocarbamate afforded alkykl N,N-dimethyldithiocarbamates inverted configuration. The latter compounds were converted to thioalcohols retention.
E-2078 is a very stable dynorphin analog that has the same affinity and selectivity for opioid receptors as dynorphin-A by in vitro bioassay. In present study, we have characterized receptor of mu-, delta- kappa-representative binding assays, evaluated analgesic effect systemically administered tail pinch, flick formalin tests mice. possessed higher kappa-receptors than mu- or delta-receptors receptor-binding assay. Dose-related, long-lasting analgesia was produced s.c. injection E-2078, its...
Enzymatic selective dephosphorylation of 1-β-D-arabinofuranosylcytosine 3', 5'-diphosphate (1) with nuclease-3'-nucleotidase P1 at 60° for 24 hr gave 5'-phosphate (Ara CMP) (2) in good yield. The acylation both N4- and the 2', 3'-hydroxyl groups 2 followed by coupling various alcohols phenols presence 2, 4, 6-triisopropylbenzenesulfonyl chloride subsequent alkaline hydrolysis afforded title compounds (4). resulting 32 kinds Ara CMP alkyl or aryl esters were examined to determine their...
Ο-Benzyltyrosine was rapidly deprotected without formation of 3-benzyltyrosine by treatment with trifluoroacetic acid containing pentamethylbenzene. This method also found to be useful in the deprotection Nε-benzyloxycarbonyllysine [Lys (Z)] and NG-4-methoxy-2, 3, 6-trimethylbenzenesulfonylarginine [Arg (Mtr)]. The new deprotecting successfully applied synthesis kyotorphin (Tyr-Arg).
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and structure-activity relationships of dynorphin A-(1-8) amide analogsHiroshi Yoshino, Takahiro Nakazawa, Yoshihiro Arakawa, Takeru Kaneko, Yutaka Tsuchiya, Manabu Matsunaga, Shin Araki, Masuhiro Ikeda, Kiyomi Yamatsu, Shinro TachibanaCite this: J. Med. Chem. 1990, 33, 1, 206–212Publication Date (Print):January 1990Publication History Published online1 May 2002Published inissue 1 January...
5′-Phosphodiesterase, which degrades RNA into nucleoside-5′-monophosphates but does not attack DNA, is present only in mycelium also culture filtrate of Penicillium citrinum Thorn 1131. For the formation this enzyme pH medium must be kept below 7.0 during culture, as inactivated rapidly alkaline solution. The optimum region 5. Cysteine, Mg++, sodium fluoride, and inorganic ortho- or pyrophosphate are without appreciable effect on enzyme. Nucleoside-5′-monophosphates, have been regarded new...
The antitumor activity of 1-beta-D-arabinofuranosylcytosine-5'-alkylphosphates (CnPCAs) against L1210 leukemia in mice after oral administration was demonstrated. optimum length the alkyl group on phosphate moiety CnPCA for exhibiting a high found to be between tetradecyl (C14) and tricosyl (C23). most active derivative this system 1-beta-D-arabinofuranosylcytosine-5'-stearylphosphate (C18PCA). minimum effective doses C18PCA were 100 6.25 mg/kg/day (q1d, day 1 5), respectively. maximum T/C%...