A5085195548- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Fibroblast Growth Factor Research
- Cell Adhesion Molecules Research
- Osteoarthritis Treatment and Mechanisms
- Carbohydrate Chemistry and Synthesis
- Connective tissue disorders research
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Liver physiology and pathology
- Silk-based biomaterials and applications
- Polysaccharides Composition and Applications
- Angiogenesis and VEGF in Cancer
- Pulmonary Hypertension Research and Treatments
- Nerve injury and regeneration
- Tendon Structure and Treatment
- Axon Guidance and Neuronal Signaling
- Collagen: Extraction and Characterization
- Viral Infectious Diseases and Gene Expression in Insects
- Congenital heart defects research
- Bone Tumor Diagnosis and Treatments
- Alzheimer's disease research and treatments
- Tissue Engineering and Regenerative Medicine
- Mast cells and histamine
- Peptidase Inhibition and Analysis
Aichi Medical University
2014-2024
Institute for Molecular Science
2006-2017
Scripps Research Institute
2011
Cleveland Clinic Lerner College of Medicine
2010
Shinshu University
2010
Molecular Oncology (United States)
2010
Kyoto Sangyo University
2010
National Center for Geriatrics and Gerontology
2010
Institute on Aging
2010
Shinshu University Hospital
2010
Three mammalian hyaluronan synthase genes, <i>HAS1</i>, <i>HAS2,</i> and <i>HAS3</i>, have recently been cloned. In this study, we characterized compared the enzymatic properties of these three HAS proteins. Expression any genes in COS-1 cells or rat 3Y1 fibroblasts yielded <i>de novo</i> formation a coat. The pericellular coats formed by HAS1 transfectants were significantly smaller than those HAS2 HAS3 transfectants. Kinetic studies enzymes membrane fractions isolated from demonstrated...
Fibronectin is a major cell-surface glycoprotein which has been reported to interact with glycosaminoglycans. A nitrocellulose filter-binding assay was developed quantitate these interactions at physiological pH and ionic strength. isolated from chick embryo fibroblasts binds both hyaluronic acid heparin; heparan sulfate bound less efficiently, chondroitin glycopeptides are minimally. The binding of heparin fibronectin saturable reversible occurs separate sites. molecules not blocked by EDTA...
This chapter focuses on the structure, biosynthesis, and general biology of proteoglycans. Topics include a description major families proteoglycans, their characteristic polysaccharide chains (glycosaminoglycans), biosynthetic pathways, concepts about proteoglycan function. Proteoglycans, like other glycoconjugates, are enormously diverse have many essential roles in biology.
Digestion of chick-embryo cartilage proteoglycan (type H) with chondroitin AC II lyase or keratanase, in the presence EDTA, N-ethylmaleimide, phenylmethanesulphonyl fluoride and pepstatin, resulted removal bulk sulphate keratan chains respectively, without altering protein portion macromolecule. An exhaustive treatment followed by digestion keratanase yielded a core fraction having enzymically modified linkage oligosaccharides. Zonal sedimentation this preparation on sucrose gradient 0.5%...
Elevated hyaluronan biosynthesis and matrix deposition correlates with cell proliferation migration. We ectopically expressed three isoforms of synthase (HAS1, HAS2, or HAS3) in nontransformed rat 3Y1 cells observed a de novo , massive formation that resulted partial loss contact-mediated inhibition growth All HAS transfectants showed an enhanced motility scratch wound assays, significant increase their confluent densities. In high-density cultures, the had fibroblastic shape markedly formed...
Heparan sulfate (HS) chains interact with various growth and differentiation factors morphogens, the most interactions occur on specific regions of certain monosaccharide sequences sulfation patterns. Here we generated a library octasaccharides by semienzymatic methods using recombinant HS 2-O-sulfotransferase 6-O-sulfotransferase, have made systematic investigation binding structures for heparin-binding factors. An octasaccharide (Octa-I, ΔHexA-GlcNSO3-(HexA-GlcNSO3)3) was prepared partial...
Hyaluronan (HA) associates with proteins and proteoglycans to form the extracellular HA-rich matrices that significantly affect cellular behaviors. So far, only heavy chains of plasma inter-α-trypsin inhibitor (ITI) family, designated as SHAPs (serum-derivedhyaluronan-associated proteins), have been shown bind covalently HA. The physiological significance such a unique covalent complex has unknown but is great interest, because HA ITI family are abundant in tissues plasma, respectively,...
Specific inhibitors of hyaluronan (HA) biosynthesis can be valuable therapeutic agents to prevent cancer invasion and metastasis. We have found previously that 4-methylumbelliferone (MU) inhibits HA synthesis in human skin fibroblasts group C Streptococcus. In this paper, the inhibition mechanism mammalian cells was investigated using rat 3Y1 stably expressing synthase (HAS) 2. Exposure transfectants inhibitor resulted significant reduction matrix formation. The evaluation HAS transcripts...
We previously cloned heparan sulfate 6-O-sulfotransferase (HS6ST) (Habuchi, H., Kobayashi, M., and Kimata, K. (1998) J. Biol. Chem. 273, 9208-9213). In this study, we report the cloning characterization of three mouse isoforms HS6ST, a homologue to original human HS6ST (HS6ST-1) two novel HS6STs (HS6ST-2 HS6ST-3). The cDNAs have been obtained from brain cDNA library by cross-hybridization with cDNA. contained single open reading frames that predicted type II transmembrane proteins composed...
We have demonstrated previously that chick embryo fibroblasts synthesize and secrete a large chondroitin sulfate proteoglycan (designated PG-M) binds to fibronectin. now report the possibility PG-M interactions with cell surfaces can modulate cell-substrate adhesion. When was added medium, various types of trypsinized cells failed adhere not only fibronectin-coated substrates but also collagen- or vitronectin-coated substrates. Adhesion laminin glycyl-arginyl-glycyl-aspartyl-serine...
Extraction of stage 22-23 chick embryo limb buds that had been metabolically labeled with [SsS]sulfate yielded heparan sulfate proteoglycan, small chondroitin and large proteoglycan (designated PG-M).PG-M constituted over 60% the total macromolecular [SsS]sulfates.It was larger in hydrodynamic size, richer protein, contained fewer chains as compared to predominant (PG-H, M,
Sequential extractions of the basement membrane producing Engelbreth-Holm-Swarm tumor yielded heparan sulfate proteoglycans with different size core proteins, but same side chains.Saline, a nondenaturing solvent, extracted small high density proteoglycan heterodisperse protein M , = 95,000-130,000 whereas subsequent extraction 7 urea, denaturing removed large, low M, 350,000-400,000 core.The conditions required for large proteogtycan suggest that it interacts strongly other...
Binding of basic fibroblast growth factor (bFGF) to the extracellular matrix cultured bovine aorta smooth muscle cells is likely be mediated via heparan sulphate, since not only exogenous addition sulphate culture medium but also pretreatment with heparitinase (but chondroitinase ABC) resulted in loss binding. Comparison affinity bFGF various glycosaminoglycan-conjugated gels showed a direct and specific binding sulphate. Heparan bound gel. However, proportion varied depending on source HS...
Vascular endothelial growth factor (VEGF) is a family of glycoproteins with potent angiogenic activity. We reported previously that heparin has an affinity for VEGF<sub>165</sub>, the major isoform VEGF, whereas 2-<i>O</i>-desulfated and 6-<i>O</i>-desulfated have weak but significant (Ashikari-Hada, S., Habuchi, H., Kariya, Y., Itoh, N., Reddi, A. Kimata, K. (2004) <i>J. Biol. Chem.</i> 279, 12346–12354). In this study, we first examined effect modified heparins (completely desulfated...