A5102814895- Viral Infectious Diseases and Gene Expression in Insects
- Silkworms and Sericulture Research
- Silk-based biomaterials and applications
- Plant Virus Research Studies
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Neurobiology and Insect Physiology Research
- Plant and Fungal Interactions Research
- Insect Utilization and Effects
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Physiological and biochemical adaptations
- Insect and Arachnid Ecology and Behavior
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- HIV/AIDS drug development and treatment
- Heat shock proteins research
- Diabetes and associated disorders
- Renal Transplantation Outcomes and Treatments
- Lipid metabolism and disorders
Sericultural Research Institute
2015-2025
Jiangsu University of Science and Technology
2015-2025
Chinese Academy of Agricultural Sciences
2015-2025
Ministry of Agriculture and Rural Affairs
2023-2025
Nanjing University of Chinese Medicine
2024
Fu Wai Hospital
2010
Western University
1998-2006
Yale University
1989-2001
Saudi Center for Organ Transplantation
1999
Harvard University Press
1999
Abstract The silkworm Bombyx mori is an important economic insect for producing silk, the “queen of fabrics”. currently available genomes limit understanding its genetic diversity and discovery valuable alleles breeding. Here, we deeply re-sequence 1,078 silkworms assemble long-read 545 representatives. We construct a high-resolution pan-genome dataset representing almost entire genomic content in silkworm. find that population harbors high density variants identify 7308 new genes, 4260...
Host–pathogen interactions are complex processes and it is a central challenge to reveal these interactions. Fungal infection of silkworm, Bombyx mori, may induce variety responsive reaction. However, little known about the molecular mechanism silkworm immune response against fungal infection. To obtain an overview interaction between entomopathogenic fungus Beauveria bassiana, Digital Gene Expression profiling, tag based high-throughput transcriptome sequencing method, was employed screen...
The silkworm is a species within the order Lepidoptera and an economic insect. nutrients are obtained from leaf utilized by larvae for body growth, development, cocoon formation. Protein plays significant functional role in diet of silkworms. To investigate impact high-protein (HPD 6%) on growth transcriptomic analysis was conducted midgut, 1724 differentially expressed genes (DEGs) were identified, comprising 803 up-regulated 921 down-regulated genes. exhibited majority pathway...
Abstract Bombyx mori Tryptophanyl-tRNA synthetase ( BmWARS ) belongs to the family of Ic-like aminoacyl-tRNA synthetases (aaRSs), whose specific recognition substrate Trp, tRNA, maintains fidelity protein synthesis. In this study, was cloned and characterized from midgut silkworm, , resulting in an open reading frame (ORF) with a full length 1,149 bp, which can encode 382 Aa. is localized cytoplasm, expressed all tissues higher expression testis, ovary, silk gland malpighian tubule. The...
CD45 is a family of transmembrane protein tyrosine phosphatases exclusively expressed by hematopoietic cells and critically involved in the regulation T cell activation signals. We now demonstrate that three 100-μg doses anti-CD45RB mAb MB23G2 can induce long-term engraftment islets into major histcompatibility complex-disparate chemically diabetic mice. Long-term graft survivors (>120 days) were tolerant to new islet allografts from original donor strain. induced temporary decrease...
Abstract The induction and maintenance of allograft tolerance is a daunting challenge. Although combined blockade CD28 CD40 ligand (CD40L)-costimulatory pathways prevents rejection in some murine models, this strategy unable to sustain engraftment the most immunogenic strain combinations. By targeting T cell activation signals 1 2 with novel combination anti-CD45RB anti-CD40L, we now demonstrate potent enhancement C57BL/6 recipients that are relatively resistant costimulatory blockade. This...
Abstract Identification of surface markers involved in osteoblast differentiation provides a method to isolate osteoblasts at various stages maturation. In this study, we examined expression the T lymphocyte antigen, Thy-1, by osteoblastic cells from different species. Murine skeletal progenitor, neonatal calvarial, and adult bone (ABCs) were selected represent distinct Flow cytometric analysis showed that Thy-1 was undetectable on progenitor (mouse limb bud clones 14 17), appeared calvarial...
Abstract QiufengN is a silkworm strain. During the feeding process of QiufengN, mutant black pupal cuticle QiufengNBP was found. Some pupae were unable to easily molt during pupation, and some eggs produced by developed normally but larvae break out eggshells. These phenomena had not been observed in other pupa mutants. Genetic analysis showed that melanization trait controlled recessive gene located on autosome follows Mendelian inheritance. Results positional cloning qRT‐PCR occurrence...
Background. Interspecies differences create important shortcomings in existing animal models used to describe vivo events responsible for allograft rejection. Alloimmune destruction of human dermal microvessels, histologically consistent with rejection, has been demonstrated skin-grafted severe combined immunodeficient (SCID) mice receiving allogeneic peripheral blood mononuclear cells (PBMC). We have now documented alloimmune injury a vascularized, SCID-human arterial transplantation model....
Purpose. To decipher transcriptomic changes and related genes with potential functions against Bombyx mori nucleopolyhedrovirus infection to increase the understanding of enhanced virus resistance silkworm on level. Methods. We assembled annotated transcriptomes Qiufeng (susceptible infection) QiufengN (resistant strains performed comparative analysis in order BmNPV infection. Results. A total 78,408 SNPs were identified strain 56,786 strain. Besides, novel AS events found these 2 strains....
The silkworm, Bombyx mori Linnaeus, is an important economic insect and a representative model organism of Lepidoptera, which has been widely used in the study reproduction development. development silkworm’s reproductive gland easily affected by many external factors, such as chemical insecticides. After silkworm larvae were treated with different concentrations pyriproxyfen, results showed that number eggs hatching rate can be reduced concentration effects displayed. Pyriproxyfen exposure...
Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the most serious pathogens restricting sustainable development sericulture industry. Currently, there no effective treatment for BmNPV infection in silkworms, and mechanism underlying resistance silkworms also not clear. In this study, comparative transcriptome analyses were carried out midguts two silkworm varieties, namely BaiyuN, which a variety, Baiyu, susceptible at five different time points (i.e., 0, 1, 3, 6, 9 h) post-BmNPV to detect...
N6-methyladenosine (m6 A) plays a key role in regulating gene expression myriad organisms. Diapause is an important plastic phenotype that allows insects to survive under specific environmental conditions. However, the diapause molecular mechanism remains unknown. In this study, we analyzed phylogenetics of genes related m6 A modification complex silkworm (Bombyx mori) based on identified sequences from other We detected these during different developmental phases four strains with...
Previously, an anti-CD45RB monoclonal antibody (mAb) has been shown to induce murine allograft tolerance. The present study was performed assess the ability of anti-human CD45RB mAb prevent rejection in a monkey MHC-mismatched kidney transplant model. recipients were allocated into following treatment groups: (1) isotype control IgG; (2) mouse IgG1 (6G3); (3) human-mouse chimeric anti-CD45RB-IgG1 (C6G3-IgG1); (4) anti-CD45RB-IgG2 (C6G3-IgG2); (5) tacrolimus at subtherapeutic dose and (6)...
Abstract Background Two species of wild silkworms, the Chinese oak silkworm ( Antheraea pernyi ) and castor Philosamia cynthia ricini , can acquire a serious disease caused by Nucleopolyhedrin Viruses (NPVs) (known as AnpeNPV PhcyNPV, respectively). The two viruses have similar polyhedral morphologies their viral fragments share high sequence similarity. However, physical maps genomes cross-infectivity are different. genome sequences isolates been published. Results We sequenced analyzed...
Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm disease that especially harmful to cocoon production and seriously restricts sericultural development. Our laboratory successfully cultivated new highly BmNPV-resistant variety, Huakang 2; however, its mechanism of BmNPV resistance remains unclear. To understand mechanism, we conducted metabolomic transcriptomic study the midgut varieties, Baiyu N after infection. We identified 451 differential metabolites, which were mostly comprised...