A5060860450- Silk-based biomaterials and applications
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Silkworms and Sericulture Research
- Plant and Fungal Interactions Research
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Invertebrate Immune Response Mechanisms
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Muscle Physiology and Disorders
- Xenotransplantation and immune response
- Organ Transplantation Techniques and Outcomes
- Insect symbiosis and bacterial influences
- Reproductive Biology and Fertility
- Ubiquitin and proteasome pathways
- Plant Virus Research Studies
- Physiological and biochemical adaptations
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Insect and Arachnid Ecology and Behavior
- Growth Hormone and Insulin-like Growth Factors
- Genetic and phenotypic traits in livestock
- Animal Nutrition and Physiology
- Liver physiology and pathology
Southwest University
2019-2024
Ministry of Agriculture and Rural Affairs
2019-2024
State Key Laboratory of Silkworm Genomic Biology
2021-2022
The Dainippon Silk Foundation
2021-2022
China Agricultural University
2018-2019
Abstract The silkworm Bombyx mori is an important economic insect for producing silk, the “queen of fabrics”. currently available genomes limit understanding its genetic diversity and discovery valuable alleles breeding. Here, we deeply re-sequence 1,078 silkworms assemble long-read 545 representatives. We construct a high-resolution pan-genome dataset representing almost entire genomic content in silkworm. find that population harbors high density variants identify 7308 new genes, 4260...
Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease the most economically important of swine industry worldwide.Highly pathogenic-PRRS virus (HP-PRRSV) variant PRRSV, which caused high morbidity mortality.Scavenger receptor CD163, contains nine scavenger cysteine-rich (SRCR) domains, key entry mediator for PRRSV.A previous study demonstrated that SRCR domain 5 (SRCR5), encoded by exon 7, was essential PRRSV infection in vitro.Here, we substituted 7 porcine...
Many insects spin cocoons to protect the pupae from unfavorable environments and predators. After emerging pupa, moths must escape sealed cocoons. Previous works identified cocoonase as active enzyme loosening cocoon form an escape-hatch. Here, using bioinformatics tools, we show that is specific Lepidoptera it probably existed before occurrence of lepidopteran spinning Despite differences in cocooning behavior, further evolved by purification selection more intense Experimentally, applied...
Abstract Feeding behavior is critical for insect survival and fitness. Most researchers have explored the molecular basis of feeding behaviors by identifying elucidating function olfactory receptors (ORs) gustatory (GRs). Other types genes, such as transcription factors, rarely been investigated, little known about their potential roles. The silkworm ( Bombyx mori ) a well‐studied monophagic which primarily feeds on mulberry leaves, but genetic its monophagy still not understood. In this...
CRISPR/Cpf1 has emerged recently as an effective tool for genome editing in many organisms, but its use pigs to generate precise genetic modifications seldom been described. Myostatin (MSTN) is a well-characterized negative regulator of muscle development, and natural mutations this gene cause double-muscled phenotype species. However, the best our knowledge, no naturally occurring mutation MSTN found pigs. In addition, living pig models with sophisticated orthologous have yet reported....
In this study, we upregulated insulin-like growth factor-1 (IGF1) expression specifically in skeletal muscle by engineering an enhancer into its non-coding regions and verified the expected phenotype a mouse model. To select appropriate site for introducing muscle-specific myosin light chain (MLC) enhancer, three candidate sites that exhibited least evolutionary conservation were chosen validated C2C12 single-cell colonies harbouring MLC at each site. IGF1 was dramatically only 2 colony...
Insect wings are subject to strong selective pressure, resulting in the evolution of remarkably diverse wing morphologies that largely determine flight capacity. However, genetic basis and regulatory mechanisms underlying size shape development not well understood. The silkworm Bombyx mori micropterous (mp) mutant exhibits shortened length enlarged vein spacings, albeit without changes total area. Thus, mp comprises a valuable resource for studying development. In this study, we used...
Abstract Background With the emergence of CRISPR/Cas9 technology, multiple gene editing procedures became available for silkworm. Although binary transgene-based methods have been widely used to generate mutants, delivery system via DNA-free ribonucleoproteins offers several advantages. However, T7 promoter that is in ribonucleoprotein-based method production sgRNAs vitro requires a 5′ GG motif efficient initiation. The resulting transcripts bear motif, which significantly constrains number...
The silkworm Bombyx mori is a domestic insect for silk production and lepidopteran model. currently available genomes limit full understanding of its genetic phenotypic diversity. Here we assembled long-read 545 wild silkworms constructed high-resolution pan-genome dataset. We found that the population harbors extremely variable containing 7,308 new gene families, 4,260 (22%) core 3,432,266 non-redundant SVs. deciphered series causal genes variants associated with domestication, breeding,...
Abstract Background: With the emergence of CRISPR/Cas9 technology, multiple gene editing procedures became available for silkworm. Although binary transgene-based methods have been widely used to generate mutants, delivery system via DNA-free ribonucleoproteins offers several advantages. However, T7 promoter that is in ribonucleoprotein-based method production sgRNAs vitro requires a 5’ GG motif efficient initiation. The resulting transcripts bear motif, which significantly constrains number...
Abstract Insect wings are subject to strong selective pressure, resulting in the evolution of remarkably diverse wing shapes that largely determine flight capacity. However, genetic basis and regulatory mechanisms underlying shape development not well understood. The silkworm Bombyx mori micropterous ( mp ) mutant exhibits shortened length enlarged vein spacings, albeit without changes total area. Thus, comprises a valuable resource for studying development. In this study, we used molecular...