A5038350600- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Porphyrin Metabolism and Disorders
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- DNA Repair Mechanisms
- Porphyrin and Phthalocyanine Chemistry
- RNA Interference and Gene Delivery
- Hemoglobin structure and function
- Heme Oxygenase-1 and Carbon Monoxide
- Enzyme Structure and Function
- RNA Research and Splicing
- Microbial Community Ecology and Physiology
- DNA and Nucleic Acid Chemistry
- Escherichia coli research studies
- Virus-based gene therapy research
- Bryophyte Studies and Records
- Advances in Cucurbitaceae Research
- Metabolism and Genetic Disorders
- Advanced biosensing and bioanalysis techniques
- Erythrocyte Function and Pathophysiology
- Legume Nitrogen Fixing Symbiosis
Yoshida Biological Laboratry
2022
Nagahama Institute of Bio-Science and Technology
2010-2015
Kyoto University
1996-2014
Fukuoka Dental College
2013
National Institute of Technology and Evaluation
2005
The University of Tokyo
1989-2002
Nara Institute of Science and Technology
2002
Tokyo University of Science
1997
University of Wisconsin–Madison
1972-1996
Tokyo Institute of Technology
1994
The Rice Annotation Project Database (RAP-DB, http://rapdb.dna.affrc.go.jp/) has been providing a comprehensive set of gene annotations for the genome sequence rice, Oryza sativa (japonica group) cv. Nipponbare. Since first release in 2005, RAP-DB updated several times along with assembly updates. Here, we present our newest based on latest assembly, Os-Nipponbare-Reference-IRGSP-1.0 (IRGSP-1.0), which was released 2011. We detected 37,869 loci by mapping transcript and protein sequences 150...
We have determined that 10Sa RNA (one of the small stable RNAs found in Escherichia coli) has an interesting structural feature: 5' end and 3' can be arranged a structure is equivalent to half-molecule (acceptor stem TFC stem-loop) alanine tRNA E. coli. Primer-extension analysis extracted from bacterial mutant with temperature-sensitive RNase P function revealed precursor (pre-10Sa RNA) folded into pre-tRNA-like vivo such it cleaved by generate mature RNA. The purified charged vitro....
The plasma membrane of mammalian cells is one the tight barriers against gene transfer by synthetic delivery systems. Various agents have been used to facilitate destabilizing endosomal under acidic conditions, but their utility limited, especially for <i>in vivo</i>. In this article, we report that protein transduction domain human immunodeficiency virus type 1 Tat (Tat peptide) greatly facilitates via destabilization. We constructed recombinant λ phage particles displaying peptide on...
DsbA, the disulfide bond catalyst of Escherichia coli , is a periplasmic protein having thioredoxin-like Cys-30-Xaa-Xaa-Cys-33 motif. The Cys-30–Cys-33 donated to pair cysteines on target proteins. Although high oxidizing potential, prone reduction, it maintained essentially all oxidized in vivo . DsbB, an integral membrane two pairs essential cysteines, reoxidizes DsbA that has been reduced upon functioning. It not known, however, what might provide overall power DsbA–DsbB formation system....
Early in the productive growth cycle following infection, λ DNA can be found doubly branched circular structures (Ogawa et al., 1968). The positions of branch points these molecules suggest that each molecule carries two replication forks moving away from a locus region genes O and P (Schnos Inman, 1970). These suggestions have been substantiated refined by measurements deletion prophages presence helper (Stevens this volume). process divided into step creating fork (initiation) subsequent...
We updated the tRNADB-CE by analyzing 939 complete and 1301 draft genomes of prokaryotes eukaryotes, 171 virus genomes, 121 chloroplast approximately 230 million sequences obtained metagenome analyses 210 environmental samples. The 287 102 tRNA genes in total, thus two times compiled previously, are compiled, which sequence information, clover-leaf structure results similarity oligonucleotide-pattern search can be browsed. In order to pool collective knowledge with help from any experts...
HemK, a universally conserved protein of unknown function, has high amino acid similarity with DNA-(adenine-N6) methyl transferases (MTases). A certain mutation in hemK gene rescues the photosensitive phenotype ferrochelatase-deficient ( hemH) mutant Escherichia coli . knockout strain E. not only suffered severe growth defects, but also showed global shift expression to anaerobic respiration, as determined by microarray analysis, and this may lead abrogation photosensitivity reducing...
Protoporphyrinogen oxidase (PPO; EC 1.3.3.4) is the enzyme that catalyzes in penultimate step heme biosynthetic pathway. Hemes are essential components of redox enzymes, such as cytochromes. Thus, a hemG mutant strain Escherichia coli deficient PPO defective aerobic respiration and grows poorly even rich medium. By complementation with human placental cDNA library, we were able to isolate clone enhanced poor growth strain. The encoded gene for PPO. Sequence analysis revealed consists 477...
We have isolated a X-transducing phage carrying the gene (glns) for Escherichia coli glutaminyl-tRNA synthetase.The location of glnS within 13.5kilobase E. DNA transducing fragment was determined by genetic means.The recloned into plasmid pBR322 and its nucleotide sequence established.The translates to protein 550 amino acids.
Complex II (succinate-ubiquinone oxidoreductase) from Escherichia coli is composed of four nonidentical subunits encoded by the sdhCDAB operon. Gene products sdhC and sdhD are small hydrophobic that anchor hydrophilic catalytic (flavoprotein iron-sulfur protein) to cytoplasmic membrane believed be components cytochrome b556 in E. complex II. In present study, elucidate role two heme b ligation functional assembly II, plasmids carrying portions sdh gene were constructed introduced into MK3,...
Mutations in the visA gene of Escherichia coli cause mutant bacteria to die upon illumination with visible light. We confirmed genetically that is a structural for ferrochelatase (protoheme ferro-lyase, EC 4.99.1.1). Since other mutations genes involved biosynthesis heme can cure photosensitivity, light-induced cell death appears be brought about by accumulation protoporphyrin IX, one substrates ferrochelatase. When cells are illuminated light, IX seems produce an active species oxygen...
CD4+ T cell plasticity plays a pivotal role in immune homeostasis. However, evidence of and its pathological human systemic lupus erythematosus (SLE) is missing due to the lack reporter system. Here we utilized receptor (TCR) repertoire data as molecular signatures alongside transcriptomic dataset. Using large-scale ImmuNexUT database autoimmune disease patients including 117 SLE cases, quantified across 13 fine-grained cell-types. We analyzed 6,392 samples total identified two orthogonal...
The first committed step of porphyrin synthesis in higher plants is the reduction glutamyl-tRNA to glutamate 1-semialdehyde. This reaction catalyzed by reductase, which encoded hemA genes. Two cDNA clones (hemA1 and hemA2) were obtained from cucumber (Cucumis sativus) cotyledons PCR library screening. They showed significant homology with published sequences. Southern blot analysis genomic DNA revealed that these genes are located at different loci there another gene similar Accumulation...