A5028679811- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Inflammatory Myopathies and Dermatomyositis
- Rheumatoid Arthritis Research and Therapies
- Atherosclerosis and Cardiovascular Diseases
- Systemic Sclerosis and Related Diseases
- Vasculitis and related conditions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammasome and immune disorders
- Chronic Lymphocytic Leukemia Research
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Immunodeficiency and Autoimmune Disorders
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Ocular Diseases and Behçet’s Syndrome
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Renal Diseases and Glomerulopathies
- Pleural and Pulmonary Diseases
- IL-33, ST2, and ILC Pathways
- Infectious Diseases and Tuberculosis
The University of Tokyo
2015-2024
University of Hildesheim
2023
Amsterdam University Medical Centers
2023
University of Amsterdam
2023
Justus-Liebig-Universität Gießen
2023
Agaplesion Markus Hospital
2023
New York University
2023
University of Gothenburg
2023
Kumamoto University
2023
RIKEN Center for Integrative Medical Sciences
2018
Systemic lupus erythematosus (SLE) is a complex autoimmune disease involving multiple immune cells. To elucidate SLE pathogenesis, it essential to understand the dysregulated gene expression pattern linked various clinical statuses with high cellular resolution. Here, we conducted large-scale transcriptome study 6,386 RNA sequencing data covering 27 cell types from 136 and 89 healthy donors. We profiled two distinct cell-type-specific transcriptomic signatures: disease-state disease-activity...
Little is known about the immunology underlying variable treatment response in rheumatoid arthritis (RA). We performed large-scale transcriptome analyses of peripheral blood immune cell subsets to identify cells that predict resistance.We isolated 18 55 patients with RA requiring addition new and 39 healthy controls, RNA sequencing. Transcriptome changes effects were systematically characterised. Association between gene modules resistance was evaluated. validated predictive value identified...
Aging is a significant risk factor for autoimmunity, and many autoimmune diseases tend to onset during adulthood. We conducted an extensive analysis of CD4
Abstract Splicing quantitative trait loci (sQTLs) are one of the major causal mechanisms in genome-wide association study (GWAS) loci, but their role disease pathogenesis is poorly understood. One reason complexity alternative splicing events producing many unknown isoforms. Here, we propose two approaches, namely integration and selection, for this by focusing on protein-structure First, integrate isoforms with same coding sequence (CDS) identify 369-601 integrated-isoform ratio QTLs (i 2...
Objective Systemic lupus erythematosus (SLE) is the prototypical systemic autoimmune disease. While long-term prognosis has greatly improved, better survival still necessary. The type I interferon (IFN) signature, a prominent feature of SLE, not an ideal therapeutic target or outcome predictor. To explore immunological pathways in SLE more precisely, we performed transcriptomic, epigenomic and genomic analyses using 19 immune cell subsets from peripheral blood. Methods We sorted identified...
Despite the involvement of B cells in pathogenesis immune-mediated diseases (IMDs), biological mechanisms underlying their function are scarcely understood. To overcome this gap, here we constructed and investigated a large-scale repertoire catalogue five cell subsets patients with IMDs.We mapped receptor regions from RNA sequencing data sorted subsets. Our dataset consisted 595 donors under IMDs health. We characterised features various aspects, including association immune transcriptomes...
B cells play a crucial role in the immune response and contribute to various autoimmune diseases. Recent studies have revealed abnormalities cell receptor (BCR) repertoire of patients with diseases, distinct features observed among different diseases subsets. Classically, BCR was used as an identifier antigen-specific clonotypes, but recent advancement analyzing large-scale has enabled us use it tool for characterizing cellular biology. In this review, we provide overview incorporating...
Regulatory T cells (Tregs) play a role in the suppression of inflammation autoimmune diseases, and lymphocyte activation gene 3 (LAG3) was reported as marker interleukin (IL)-10-producing Tregs. We aimed to clarify function human IL-10-producing CD4+CD25-LAG3+ (LAG3+ Tregs) their association with rheumatoid arthritis (RA).LAG3+ Tregs peripheral blood mononuclear (PBMCs) were cultured B follicular helper examine antibody effects. The frequency LAG3+ evaluated samples from 101 healthy donors...
Abstract Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease that leads to destructive arthritis. Although the HLA class II locus strongest genetic risk factor for rheumatoid arthritis, relationship between alleles and lymphocyte activation remains unclear. We performed immunophenotyping of peripheral blood mononuclear cells on 91 HLA-DRB1-genotyped RA patients 110 healthy donors. The frequency memory CXCR4 + CD4 T not Th1 Th17 cells, was significantly associated with...
CD4+ T cell plasticity plays a pivotal role in immune homeostasis. However, evidence of and its pathological human systemic lupus erythematosus (SLE) is missing due to the lack reporter system. Here we utilized receptor (TCR) repertoire data as molecular signatures alongside transcriptomic dataset. Using large-scale ImmuNexUT database autoimmune disease patients including 117 SLE cases, quantified across 13 fine-grained cell-types. We analyzed 6,392 samples total identified two orthogonal...
Objectives: Acute or subacute exacerbations are recognized as a severe complication of rheumatoid arthritis-associated interstitial lung disease (RA-ILD). Nevertheless, the role intensive immunosuppression in RA-ILD remains elusive. We attempted to evaluate clinical characteristics and efficacy immunosuppressive treatment exacerbated RA-ILD. Methods: Clinical data, including respiratory function, imaging, treatment, prognosis, were retrospectively collected for 17 patients with who required...
Obesity is associated with worse disease activity and drug responses in patients rheumatoid arthritis (RA). However, the immunological mechanisms responsible for relationship between RA obesity have not yet been clarified detail. This study aimed to elucidate contributing pathogenesis of overweight patients.The frequencies CD4+ T cell, B cell monocyte subsets were analyzed (n = 81) healthy donors 99) by flow cytometry, compared three groups (body mass index (BMI) <20, ≥20 25, >25). Serum...
Abstract Neutrophil extracellular traps (NETs) are involved in systemic lupus erythematosus (SLE). We sought to cluster SLE patients based on serum NET levels. Serum levels were higher than healthy controls. Frequencies of pleuritis and myositis increased with high negatively correlated anti–double stranded DNA (anti-dsDNA) antibody titers C1q-binding immune complexes, but positively C-reactive protein (CRP) monocyte counts. transcriptome analysis demonstrated no difference NET-associated...
Idiopathic inflammatory myopathies (IIM) demonstrate characteristic clinical phenotypes depending on the myositis-specific antibody (MSAs) present. We aimed to identify common or MSA-specific immunological pathways in different immune cell types from peripheral blood by transcriptome analysis.We recruited 33 patients with IIM who were separated into following groups: 15 active disease at onset and 18 inactive under treatment. All positive for MSAs: anti-melanoma differentiation-associated...
Abstract HLA-DRB1 shared epitope risk alleles are the strongest genetic factors for rheumatoid arthritis (RA) and potential biomarkers treatment response to biological disease-modifying antirheumatic drugs (bDMARDs). This study aimed investigate association between individual in RA patients receiving different bDMARDs. We recruited 106 with active who had started abatacept, tocilizumab, or TNF inhibitors as a first-line examined relationship Simplified Disease Activity Index (SDAI)...
Objective. Shared epitope (SE) alleles are the most significant genetic susceptibility locus in rheumatoid arthritis (RA); however, their target populations CD4+ T cells not well elucidated. We analyzed association between SE and cell receptor (TCR) repertoire diversity of naive memory using next-generation sequencing (NGS). Methods. The TCR beta chains from peripheral blood 22 patients with RA 18 age- sex-matched healthy donors (HD) were by NGS. Renyi entropy was used to evaluate its...
Systemic lupus erythematosus (SLE) is an autoimmune disease that involves multiple immune cell subsets. We analyzed subsets in human peripheral blood mononuclear cells (PBMC) order to identify the are significantly associated with SLE activity and treatment. The frequencies of various CD4+ T cells, B monocytes NK PBMC were assessed 30 healthy controls (HC), rheumatoid arthritis (RA) patients 26 using flow cytometry. correlations between subset clinical traits including Lupus Erythematosus...