A5076039190- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Immune cells in cancer
- Lymphoma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Fungal and yeast genetics research
- Cancer Genomics and Diagnostics
- Genomics and Chromatin Dynamics
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Plant tissue culture and regeneration
- Drug Transport and Resistance Mechanisms
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Radiopharmaceutical Chemistry and Applications
- Chronic Lymphocytic Leukemia Research
- Cancer-related Molecular Pathways
- Acute Myeloid Leukemia Research
Yokohama University of Pharmacy
2018
Roche (Switzerland)
2000-2002
Roche (Japan)
1999
Ochanomizu University
1990-1992
Otsuka (Japan)
1992
Understanding of the firing time determination replication origins in entire genome will require a genome-wide survey and their mapping on chromosomes. A microarray technology was applied to obtain profile DNA classify early origins.A total 260 potential (PROs) were identified budding yeast genome: 247 as defined peaks 13 regions located chromosomal termini. Based time, PROs classified into 143 104 late PROs, that not randomly distributed chromosomes but formed separated clusters. Most found...
Abstract Steroid and xenobiotic receptor (SXR) or human pregnane X (hPXR) has been shown to play an important role in the regulation of genes related detoxification, such as cytochrome P450 3A4 multidrug resistance gene 1. Cytochrome enzymes, conjugation transporters are all considered be involved breast carcinoma chemotherapeutic endocrine agents. However, expression SXR/hPXR proteins that its target their biological clinical significance have not examined carcinomas. Therefore, we first 60...
The final destination of glycosylphosphatidylinositol (GPI)-attached proteins in Saccharomyces cerevisiae is the plasma membrane or cell wall. Two kinds signals have been proposed for their cellular localization: (i) specific amino acid residues V, I, L at site 4 5 acids upstream GPI attachment (the omega site) and Y N 2 wall localization (ii) dibasic region omega-minus region) localization. relationships between these efficiencies incorporation were examined by constructing fusion reporter...
Background Nedd4 is a ubiquitin‐protein ligase containing calcium/lipid‐binding domain, multiple WW domains and C‐terminal Hect which required for both the ubiquitin transfer association with E2 ubiquitin‐conjugating enzymes. has been reported to be involved in selective ubiquitination of some regulatory proteins transcription membrane transport. Results Three mRNA species human were found 6.4‐, 7.8‐ 9.5‐kb size, their expression patterns varied among normal tissues cancer cell lines,...
Abstract Overcoming resistance to immune checkpoint inhibitors is an important issue in patients with non‐small‐cell lung cancer (NSCLC). Transcriptome analysis shows that adenocarcinoma can be divided into three molecular subtypes: terminal respiratory unit (TRU), proximal proliferative (PP), and inflammatory (PI), squamous cell carcinoma (LUSQ) four. However, the immunological characteristics of these subtypes are not fully understood. In this study, we investigated landscape NSCLC tissues...
Therapeutic antibodies sometimes elicit anti-drug (ADAs) that can affect efficacy and safety. Engineered contain artificial amino acid sequences are potentially highly immunogenic, but this is currently difficult to predict. Therefore, it important efficiently assess immunogenicity during the development of complex antibody-based formats. Here, we present an in vitro peripheral blood mononuclear cell-based assay be used potential within 3 days. This method involves examining frequency...
Abstract: Rapid degradation of wild-type p53 in the human uterine cervix is induced by infection high-risk papilloma virus (HPV) types 16 and 18. HPV-E6 protein plays a critical role poly-ubiquitination mediating association with E6-associated (E6AP). As result, poly-ubiquitinated rapidly selectively degraded 26S proteasome. We have established high throughput assay system to monitor using new fluorescence homogeneous technology known as Homogeneous Time-Resolved Fluorescence (HTRF™). The...
Resistance to immune checkpoint blockade remains challenging in patients with non-small cell lung cancer (NSCLC). Tumor-infiltrating leukocyte (TIL) quantity, composition, and activation status profoundly influence responsiveness immunotherapy. This study examined the landscape NSCLC tumor microenvironment by analyzing TIL profiles of 281 fresh resected tissues. Unsupervised clustering based on numbers percentages 30 types classified adenocarcinoma (LUAD) squamous carcinoma (LUSQ) into cold,...
The PHO85 gene is a negative regulator of the PHO system in yeast Saccharomyces cerevisiae and encodes protein kinase (Pho85) highly homologous to Cdc28 (Cdc28). Ten cyclin-like proteins are known interact with Pho85, combination different cyclins believed be responsible for distinct Pho85 functions, including phosphate metabolism, carbon source utilization cell cycle regulation. However, only limited number substrates kinase, Pho4, Gsy2 Sicl, have so far been identified. To search more...
Assessing how gene expression analysis by RNA sequencing (RNA-Seq) correlates to a unique morphology is increasingly necessary, and laser capture microdissection (LCM) critical research tool for discovering the genes responsible in region of interest (ROI). Because RNA-Seq requires high-quality RNA, sample preparation procedure that can preserve give required quality essential. A PAXgene®-fixed paraffin-embedded (XFPE) block satisfy need but there are few reports on adapting method LCM, such...
<p>Percentage of PD-1+ CD8 T+ and PD-L1+ cells in respective immune subtypes. (a, b) Data were presented as a percentage (a) (b) per indicated CD8+ T cell subset LUAD LUSQ. % naïve CD8; or total cells. CM EM EMRA ns; not significant. * p<0.05. **P<0.01.</p>
<p>Percentage of PD-1+ CD8 T+ and PD-L1+ cells in respective immune subtypes. (a, b) Data were presented as a percentage (a) (b) per indicated CD8+ T cell subset LUAD LUSQ. % naïve CD8; or total cells. CM EM EMRA ns; not significant. * p<0.05. **P<0.01.</p>
<p>Antibodies for IHC</p>