A5009564963- Escherichia coli research studies
- Sulfur Compounds in Biology
- Viral gastroenteritis research and epidemiology
- Nitric Oxide and Endothelin Effects
- Endoplasmic Reticulum Stress and Disease
- Adenosine and Purinergic Signaling
- Vibrio bacteria research studies
- Inflammasome and immune disorders
- Eicosanoids and Hypertension Pharmacology
- Heme Oxygenase-1 and Carbon Monoxide
- Nanoparticles: synthesis and applications
- Helicobacter pylori-related gastroenterology studies
- Bacterial Genetics and Biotechnology
- Redox biology and oxidative stress
- Nanoparticle-Based Drug Delivery
- Transgenic Plants and Applications
- Advanced Glycation End Products research
- Nanoplatforms for cancer theranostics
- Antimicrobial agents and applications
- Gut microbiota and health
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Antibiotic Resistance in Bacteria
- Botulinum Toxin and Related Neurological Disorders
Kumamoto University
2015-2024
Tokyo Medical and Dental University
2019
Osaka Prefecture University
2006-2016
Chiba University
2010-2014
Fusobacterium nucleatum (F. nucleatum) is a gut microbe implicated in gastrointestinal tumorigenesis. Predicting the chemotherapeutic response critical to developing personalised therapeutic strategies for oesophageal cancer patients. The present study investigated relationship between F. and resistance squamous cell carcinoma (ESCC).We examined chemotherapy 120 ESCC resected specimens 30 pre-treatment biopsy specimens. In vitro studies using lines co-culture assays further uncovered...
The NLRP3 inflammasome is a multiprotein complex responsible for the maturation of precursor forms interleukin (IL)-1β and IL-18 into active proinflammatory cytokines. Increasing evidence suggests that modulation redox homeostasis contributes to activation inflammasome. However, specific mechanistic details remain unclear. We demonstrate here ATP exposure evoked sharp decrease in glutathione (GSH) levels macrophages, which led activation. detected an increase GSH culture supernatants was...
Abstract Innate immunity plays an important role in host defense against microbial infections. It also participates activation of acquired through cytokine production and antigen presentation. Pattern recognition receptors such as Toll-like nucleotide oligomerization domain-like sense invading pathogens associated tissue injury, after which inflammatory mediators pro-inflammatory cytokines nitric oxide are induced. Supersulfides molecular species possessing catenated sulfur atoms persulfide...
Summary In enterohaemorrhagic Escherichia coli (EHEC) O157, there are two types of anaerobic nitric oxide (NO) reductase genes, an intact gene ( norV ) and a 204 bp deletion norVs ). Epidemiological analysis has revealed that ‐type EHEC more virulent than EHEC. Thus, to reveal the role NO during infection, we constructed isogenic mutant strains. Under conditions, was protected from NO‐mediated growth inhibition, while strain not, suggesting NorV effective in detoxification. We then...
Abnormal activation of astrocytes (e.g., the overproduction cytokines and nitric oxide) is relevant to neurodegenerative disease. It important, therefore, search for inhibitors abnormal that can be derived from natural substances. This study focused on effects extracts young fruits Citrus unshiu lipopolysaccharide (LPS)-induced oxide (NO) production inducible synthase (iNOS) expression in rat primary astrocytes. The methanol extract citrus inhibited NO a concentration-dependent manner. After...
ABSTRACT Autophagy and apoptosis play critical roles in cellular homeostasis survival. Subtilase cytotoxin (SubAB), produced by non-O157 type Shiga-toxigenic Escherichia coli (STEC), is an important virulence factor disease. SubAB, a protease, cleaves specific site on the endoplasmic reticulum (ER) chaperone protein BiP/GRP78, leading to ER stress, induces apoptosis. Here we report that HeLa cells, activation of PERK (RNA-dependent kinase [PKR]-like kinase)-eIF2α (α subunit eukaryotic...
Hydrogen sulfide (H2S) formed during sulfur metabolism in bacteria has been implicated the development of intrinsic resistance to antibacterial agents. Despite conversion H2S hydropersulfides greatly enhancing biochemical properties such as antioxidant activity, effects on antibiotic have remained unknown. In this work, we investigated alone or together with cystine form cysteine hydropersulfide (CysSSH) activities By using disc diffusion test, found that CysSSH treatment effectively...
The botulinum neurotoxin light chain (BoNT-LC) is a zinc-dependent metalloprotease that cleaves neuronal SNARE proteins such as SNAP-25, VAMP2, and Syntaxin1. This cleavage interferes with the neurotransmitter release of peripheral neurons results in flaccid paralysis. SNAP, VAMP, Syntaxin are representative large families mediate most membrane fusion reactions, well both non-neuronal exocytotic events eukaryotic cells. Neuron-specific proteins, which target substrates BoNT, have been...
Ferulic acid (FA) has been reported to exhibit protective effects against amyloid-β (Aβ)-induced neurodegeneration in vitro and vivo. Recently, we developed two water-soluble FA derivatives: 1-feruloyl glycerol diglycerol. In this study, examined the neuroprotective of these derivatives on Aβ-induced both inhibited Aβ aggregation destabilized pre-aggregated a similar extent. Furthermore, derivatives, as well FA, neuronal cell death cultured cells. vivo experiments, oral administration mice...
Subtilase cytotoxin (SubAB), which is produced by certain strains of Shiga-toxigenic Escherichia coli (STEC), cleaves an endoplasmic reticulum (ER) chaperone, BiP/Grp78, leading to induction ER stress and caspase-dependent apoptosis. SubAB alters the innate immune response. pretreatment macrophages inhibited lipopolysaccharide (LPS)-induced production both monocyte chemoattractant protein 1 (MCP-1) tumor necrosis factor α (TNF-α). We investigated here mechanism inhibits nitric oxide (NO)...
8-Nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP) is a nitrated derivative of guanosine (cGMP) formed endogenously under conditions associated with production both reactive oxygen species and nitric oxide. It acts as an electrophilic second messenger in the regulation cellular signaling by inducing post-translational modification redox-sensitive protein thiols via covalent adduction cGMP moieties to (protein S-guanylation). Here, we demonstrate that 8-nitro-cGMP potentially...
Silver nanoparticles have antibacterial activity. However, the are unstable and easily form aggregates, which decreases their To improve dispersion stability of silver in aqueous media to increase effectiveness as agents, we coated triangular plate-like (silver nanoplates, Ag NPLs) with one or two layers gold atoms (Ag@Au1L NPLs Ag@Au2L NPLs, respectively). These coatings improved high salt concentrations. Ag@Au1L showed stronger activity on pathogenic bacteria than NPLs. Furthermore,...
Subtilase cytotoxin (SubAB) is mainly produced by locus of enterocyte effacement (LEE)-negative strains Shiga-toxigenic Escherichia coli (STEC). SubAB cleaves an endoplasmic reticulum (ER) chaperone, BiP/Grp78, leading to induction ER stress. This stress causes activation sensor proteins and caspase-dependent apoptosis. We found that induces granules (SG) in various cells. Aim this study was explore the mechanism which induced SG formation. Here, we show SubAB-induced formation regulated...
Abstract Interferons (IFNs) are cytokines produced and secreted by immune cells when viruses, tumour cells, so forth, invade the body. Their biological effects diverse, including antiviral, cell growth-inhibiting, antitumour effects. The main subclasses of IFNs include type-I (e.g. IFN-α IFN-β) type-II (IFN-γ), which activate intracellular signals binding to IFN receptors, respectively. We have previously shown that macrophages treated with supersulphide donors, polysulphide structures in...
ABSTRACT Subtilase cytotoxin (SubAB), which is produced by certain strains of Shiga-toxigenic Escherichia coli (STEC), causes the 78-kDa glucose-regulated protein (GRP78/BiP) cleavage, followed induction endoplasmic reticulum (ER) stress, leading to caspase-dependent apoptosis via mitochondrial membrane damage Bax/Bak activation. The purpose present study was identify SubAB receptors responsible for HeLa cell death. Four proteins, NG2, α2β1 integrin (ITG), L1 adhesion molecule (L1CAM), and...
The novel cytotoxic factor subtilase cytotoxin (SubAB) is produced mainly by non-O157 Shiga-toxigenic Escherichia coli (STEC). SubAB cleaves the molecular chaperone BiP/GRP78 in endoplasmic reticulum (ER), leading to activation of RNA-dependent protein kinase (PKR)-like ER (PERK), followed caspase-dependent cell death. However, uptake mechanism HeLa cells unknown. In this study, a variety inhibitors and siRNAs were employed characterize process. SubAB-induced BiP cleavage was inhibited high...