A5034758525- Ion Channels and Receptors
- Biochemical Analysis and Sensing Techniques
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Olfactory and Sensory Function Studies
- Advanced Chemical Sensor Technologies
- RNA Interference and Gene Delivery
- Pain Mechanisms and Treatments
- Adenosine and Purinergic Signaling
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Asthma and respiratory diseases
- Neuroendocrine regulation and behavior
- Hearing, Cochlea, Tinnitus, Genetics
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Neurobiology and Insect Physiology Research
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Genetics and Neurodevelopmental Disorders
- Extracellular vesicles in disease
- IL-33, ST2, and ILC Pathways
- Genetic and Kidney Cyst Diseases
- Retinal Development and Disorders
Nagoya City University
2015-2025
Mizuho (Japan)
2001-2021
Nippon Shinyaku (Japan)
2014-2017
Japan Society for the Promotion of Science
2006
Seiko Holdings (Japan)
2004
Weatherford College
2001
Osaka City University
2000
Osaka University
1998-2000
Many painful inflammatory and ischemic conditions such as rheumatoid arthritis, cardiac ischemia, exhausted skeletal muscles are accompanied by local tissue acidosis. In acidotic states, extracellular protons provoke the pain opening cation channels in nociceptors. It is generally believed that a vanilloid receptor subtype-1 (VR1) an acid-sensing ion channel (ASIC) mediate greater part of acid-induced nociception mammals. Here we provide evidence for involvement both acid-evoked humans show...
Many painful inflammatory and ischemic conditions such as rheumatoid arthritis, cardiac ischemia, exhausted skeletal muscles are accompanied by local tissue acidosis. In acidotic states, extracellular protons provoke the pain opening cation channels in nociceptors. It is generally believed that a vanilloid receptor subtype-1 (VR1) an acid-sensing ion channel (ASIC) mediate greater part of acid-induced nociception mammals. Here we provide evidence for involvement both acid-evoked humans show...
Bitter taste perception is a conserved chemical sense against the ingestion of poisonous substances in mammals. A multigene family G-protein-coupled receptors, T2R (so-called TAS2R or TRB) receptors and G-protein alpha subunit (Galpha), gustducin, are believed to be key molecules for its perception, but little known about molecular basis interaction. Here, we use heterologous expression system determine specific domain gustducin necessary coupling. Two chimeric Galpha16 proteins harboring 37...
This study aimed to establish a rapid and simple method for isolating exosomes from raw bovine milk compare the quality of isolated with those by standard involving ultracentrifugation (UC).To remove caseins, which are major proteins consisting more than 80% protein (35% in human breast milk) hamper isolation purification exosomes, hydrochloride (HCl) was added isoelectric precipitation (IP). The effects acidification on morphological features, particle size distribution, surface charge,...
Bovine milk extracellular vesicles (EVs) attract research interest as carriers of biologically active cargo including miRNA from donor to recipient cells facilitate intercellular communication. Since toxicity edible seems be negligible, EVs are applicable use for therapeutics in human medicine. Casein separation is an important step obtaining pure milk, and recent studies reported that adding hydrochloric acid (HCl) acetic (AA) accelerates casein aggregation precipitation EV isolation...
The transient receptor potential melastatin subfamily (TRPM), which is a mammalian homologue of cell death-regulated genes in Caenorhabditis elegans and Drosophila, has roles the process cycle regulation Ca(2+) signaling. Among this subfamily, TRPM8 (also known as Trp-p8) Ca(2+)-permeable channel that was originally identified prostate-specific gene upregulated tumors. Here we showed expressed human melanoma G-361 cells, activation produced sustainable influx. application menthol, an agonist...
Acid-sensing ion channel-2a (ASIC2a) is an amiloride-blockable proton-gated cation channel, probably contributing to sour-taste detection in rat taste cells. To isolate another subtype of the receptor, we screened a circumvallate papilla cDNA library and identified ASIC2b, N-terminal splice variant ASIC2a. Reverse transcription-PCR analyses confirmed expression ASIC2b transcripts and, moreover, demonstrated its foliate fungiform papillae. Immunohistochemical revealed that as well ASIC2a, was...
We studied the localization and physiological functions of transient receptor potential (TRP) channels TRPV1 (TRP vanilloid 1) TRPV4 4) in mouse bladder, because both are thought to be mechanosensors for bladder distention. RT-PCR specifically amplified transcripts from urothelial cells, whereas were barely detectable. ISH experiments showed that abundantly expressed urothelium, not detectable cells. Immunoblotting IHC studies proteins mainly localized at basal plasma membrane domains In...
No AccessJournal of UrologyInvestigative Urology1 Apr 2014Expression Profiling microRNA in Cryptorchid Testes: miR-135a Contributes to the Maintenance Spermatogonial Stem Cells by Regulating FoxO1 Yoshinobu Moritoki, Yutaro Hayashi, Kentaro Mizuno, Hideyuki Kamisawa, Hidenori Nishio, Satoshi Kurokawa, Shinya Ugawa, Yoshiyuki Kojima, and Kenjiro Kohri MoritokiYoshinobu Moritoki Department Nephro-Urology, Graduate School Medical Sciences, Nagoya City University, Nagoya, Japan , HayashiYutaro...
New neurons, referred to as neuroblasts, are continuously generated in the ventricular-subventricular zone of brain throughout an animal's life. These neuroblasts characterized by their unique potential for proliferation, formation chain-like cell aggregates, and long-distance high-speed migration through rostral migratory stream (RMS) toward olfactory bulb (OB), where they decelerate differentiate into mature interneurons. The dynamic changes ultrastructural features postnatal-born during...
The concept of kokumi, which refers to an enhanced and more delicious flavor food, has recently generated considerable interest in food science. However, kokumi not been well studied gustatory physiology, the underlying neuroscientific mechanisms remain largely unexplored. Our previous research demonstrated that ornithine (L-ornithine), is abundant shijimi clams, taste preferences mice. present study aimed build on these findings investigate responsible for rats. In two-bottle preference...
Abstract We found that neuropsin, an extracellular matrix serine protease, has a regulatory effect on Schaffer‐collateral long‐term potentiation (LTP) in the mouse hippocampus. Bath application of 1–170 n m recombinant neuropsin modulated early phase LTP pathway with ‘bell‐shape’ dose–response curve. The maximum enhancing activity (134% control LTP) was at ∼ 2.5 . neutralizing antibody against hippocampal slice resulted marked inhibition tetanus‐induced LTP. vivo continuous intraventricular...
The subtype 2 and 3 ionotropic purinergic receptors (P2X receptors) are crucial for gustation, but the distribution of these in geniculate ganglion (GG) their colocalization tongue papillae remain unknown. Here we investigated expression P2X(2) P2X(3) GG fungiform rats mice by using situ hybridization immunohistochemistry. In both species, transcripts immunoreactivity were detected approximately 50-60% neuronal somata, whereas those observed almost all neurons. each papilla, was mostly...
The Cav3.2 isoform of the T-type calcium channel is expressed in primary sensory neurons dorsal root ganglion (DRG), and these channels contribute to nociceptive neuropathic pain rats. However, there are conflicting reports on roles processing rats mice. In addition, function persistent inflammatory hyperalgesia poorly understood. We performed behavioral comprehensive histochemical analyses characterize Cav3.2-expressing DRG examined regulation DRGs from C57BL/6 mice with carrageenan-induced...
The amiloride-sensitive epithelial Na+ channel (ENaC) controls transport into cells and across epithelia. So far, four homologous subunits of mammalian ENaC have been isolated are denoted as α, β, γ, δ. ENaCδ can associate with β γ generate a constitutive current that is 2 orders magnitude larger than homomeric ENaCδ. However, the distribution pattern not consistent subunits. expressed mainly in brain contrast to subunits, which non-neuronal tissues. To explain this discrepancy, we searched...