A5002423375- Advanced Radiotherapy Techniques
- Radiation Therapy and Dosimetry
- Head and Neck Cancer Studies
- Advanced MRI Techniques and Applications
- Lung Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Hepatocellular Carcinoma Treatment and Prognosis
- Esophageal Cancer Research and Treatment
- Head and Neck Surgical Oncology
- Lung Cancer Treatments and Mutations
- Gastric Cancer Management and Outcomes
- 3D Printing in Biomedical Research
- Cancer, Hypoxia, and Metabolism
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Advanced NMR Techniques and Applications
- Medical Imaging Techniques and Applications
- Radiation Detection and Scintillator Technologies
- NMR spectroscopy and applications
- Immune Cell Function and Interaction
- Pancreatic and Hepatic Oncology Research
- Esophageal and GI Pathology
- Boron Compounds in Chemistry
- Brain Metastases and Treatment
- Ocular Oncology and Treatments
- Ultrasound and Hyperthermia Applications
Medipolis Medical Research Institute
2015-2024
Kyushu University
1995-2024
Nagoya City University
2015-2024
National Institute for Physiological Sciences
2024
National Heart Lung and Blood Institute
2024
National Institutes of Health
2024
Universitat de València
2024
Instituto de Salud Carlos III
2024
Shiga University of Medical Science
2024
Kagoshima University
2023
PURPOSE: To investigate the efficacy and feasibility of concurrent chemoradiotherapy for locally advanced carcinoma esophagus. PATIENTS AND METHODS: Fifty-four patients with clinically T4 and/or M1 lymph node (LYM) squamous cell esophagus were enrolled. Patients received protracted infusion fluorouracil 400 mg/m 2 /24 hours on days 1 to 5 8 12, 2-hour cisplatin 40 8, radiation therapy at a dose 30 Gy in 15 fractions over 3 weeks. Filgrastim was prophylactically administered 35 patients. This...
Purpose: To assess the long-term toxicity after definitive chemoradiotherapy (CRT) for squamous cell carcinoma (SCC) of esophagus. Patients and Methods: newly diagnosed with SCC esophagus treated CRT between 1992 1999 in our institution were recruited from database on basis following criteria: age ≤ 75 years, performance status (PS; based Eastern Cooperative Oncology Group scale) 0 to 2, clinical tumor-node-metastasis system stage I IVA. The consisted two cycles cisplatin 40 mg/m 2 days 1 8,...
1H NMR spectra at 360.13 MHz were obtained of the rat brain in vivo by using a surface coil placed over skull. Resonances numerous metabolites identified comparison with excised tissue and acid extracts tissue. Changes cerebral lactate levels resulting from administration gas mixtures low oxygen monitored time resolution 2.3 min. The electroencephalogram electrocardiogram recorded simultaneously during experiment. Reversibility hypoxic response was documented when, upon administration,...
Abstract The 1 H NMR spectrum of the macromolecule fraction rat brain cytosol was investigated following centrifugation and dialysis to remove low molecular weight metabolites peptides (<3500 daltons). At least seven well resolved resonances were detected between 0.9 3.0 ppm in after dialysis, several which cannot be observed vivo due overlap with N ‐acetylaspartate, glutamate, glutamine, creatine, yaminobutyric acid. Several cross‐peaks 2D COSY spectra cytosolic coincided those measured...
Purpose To evaluate the safety and efficacy of proton beam radiotherapy (PRT) for hepatocellular carcinoma. Patients Methods Eligibility criteria this study were: solitary carcinoma (HCC); no indication surgery or local ablation therapy; ascites; age ≥ 20 years; Zubrod performance status 0 to 2; serious comorbidities other than liver cirrhosis; written informed consent. PRT was administered in doses 76 cobalt gray equivalent fractions 5 weeks. No patients received transarterial...
Abstract The changes in 16 cerebral metabolites produced by cardiac arrest and subsequent room temperature auto‐lysis were studied using high‐resolution proton nuclear magnetic resonance spectroscopy. Biopsies of rabbit cortex, white matter, cerebellum quantitatively analyzed for acetate, alanine, γ‐aminobu‐tyric acid, creatine, glutamate, glycine, inositol, lactate, N‐ acetylaspartate, phosphocreatine, succinate, taurine, threonine. Of these, N ‐acetylaspartate the total creatine pool are...
Abstract Two‐dimensional shift‐correlated (COSY) and J ‐resolved NMR spectroscopy was used to identify assign resonances in the aliphatic region (0.8 4.5 ppm) of 1 H spectrum acid extracts tissue rat brain. The chemical shift spin‐spin coupling constants several resonances, which could not be resolved one‐dimensional spectra tissue, were determined. These properties, together with appropriate multiplet structure scalar patterns observed 2D COSY spectra, lactate, threonine, alanine, γ‐amino...
New neurons, continuously added in the adult olfactory bulb (OB) and hippocampus, are involved information processing neural circuits. Here, we show that synaptic pruning of adult-born neurons by microglia depends on phosphatidylserine (PS), whose exposure dendritic spines is inversely correlated with their input activity. To study role PS spine vivo, developed an inducible transgenic mouse line, which exposed masked a dominant-negative form milk fat globule-EGF-factor 8 (MFG-E8),...
Proton therapy is one form of radiotherapy in which the irradiation can be concentrated on a tumor using scanned or modulated Bragg peak. Therefore, it very important to evaluate proton‐irradiated volume accurately. The confirmed by detection pair annihilation gamma rays from positron emitter nuclei generated target nuclear fragment reaction irradiated proton and emission tomography (PET) apparatus, dose‐volume delivery guided (DGPT) thereby achieved PET images. In treatment room, beam...
Transcatheter arterial chemoembolization (TACE) is used in the treatment of hepatocellular carcinoma; however, it has limited effect on portal vein tumor thrombus (PVTT). The purpose this study was to assess feasibility and efficacy radiotherapy targeting PVTT after TACE for hepatic parenchyma. performed using epirubicin hydrochloride, iodized poppy seed oil, gelatin sponge particles. Radiotherapy a total dose 50 Gy 25 fractions during 5 weeks. Twenty consecutive patients were treated with...
With the development of chemoradiotherapy for esophageal cancer, complete response (CR) rate would become an important surrogate end-point. However, Response Evaluation Criteria in Solid Tumors (RECIST) do not provide criteria a at primary site cancer. The objective this study was to assess validity endoscopic CR cancer treated with chemoradiotherapy.We reviewed 139 patients T1-T4, N0-1, M0-1a definitive from August 1992 April 1999. tentatively defined upon observation entire esophagus as:...
Abstract Using a 1.89‐Tesla spectrometer, 1 H and 31 P nuclear magnetic resonance spectra were acquired from the brains of paralyzed rabbits ventilated with 30% oxygen in nitrous oxide. Intracellular pH changes lactate concentration cerebrum monitored by methods during after bicuculline‐induced seizures, together electroencephalogram, heart rate, arterial blood pressure. During seizures lasting more than an hour, cerebral intracellular became acidic, level rose rapidly, both persisted as...
Abstract High‐resolution proton magnetic resonance spectroscopy was used to analyze human cerebrospinal fluid obtained from patients with several neurological problems. The major metabolites measured included glucose, lactate, glutamine, citrate, inositol, acetate, creatine, creatinine, β‐hydroxybutyrate, alanine, and pyruvate. A drug vehicle, propylene glycol, also measured. Alterations in the of these provided information concerning metabolism brain. Magnetic offered a simple rapid means...
The phenomenon of intracellular pH (pHin) regulation in cultured Ehrlich ascites cells was investigated using 31P nuclear magnetic resonance (NMR) spectroscopy. Measurements were made with a Bruker WH 360 wide bore NMR spectrometer at frequency 145.78 MHz. Samples density 10(8) ml-1 suspended final volume 2 ml growth medium 10 mm diameter tubes. Intracellular calculated from the chemical shifts either inorganic phosphate (Piin) or 2-deoxyglucose-6-phosphate (2dG6Pin). sugar used as probe to...
13C and 31P NMR spectra of wild-type Escherichia coli showed resonances from metabolic intermediates glycolysis ATP formation but no detectable signals aromatic amino acids. However, tyrosine biosynthesis D-[l-13C]glucose was observed in cells harboring a feedback-resistant allele aroF, the gene encoding tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase [7-phospho-2-keto-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), EC4.1.2.15],...
The relationship between efflux and influx of K+, Na+, intracellular pH (pHin) in yeast cells upon energizing by oxygenation was studied using the noninvasive technique 39K, 23Na, 31P NMR spectroscopy. By introducing an anionic paramagnetic shift reagent, Dy3+(P3O5(-10))2, into medium, signals intra- extracellular K+ Na+ could be resolved, enabling us to study ion transport processes NMR. Measurements showed that 40% contributed intensities. applying this correction factor, concentrations...
Abstract Although malignant phenotypes of triple-negative breast cancer (TNBC) are subject to circadian alterations, the role stem cells (CSC) in defining this change remains unclear. CSC often characterized by high aldehyde dehydrogenase (ALDH) activity, which is associated with malignancy and used for identification isolation CSC. Here, we show that population ALDH-positive a mouse 4T1 tumor model exhibits pronounced alterations. Alterations number were generated time-dependent increases...