A5060119598- Lymphatic System and Diseases
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Lymphatic Disorders and Treatments
- Immunotherapy and Immune Responses
- HER2/EGFR in Cancer Research
- Chemokine receptors and signaling
- Galectins and Cancer Biology
- T-cell and B-cell Immunology
- Cancer Cells and Metastasis
- Proteoglycans and glycosaminoglycans research
- Veterinary Oncology Research
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Vascular Malformations and Hemangiomas
- Glioma Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Lymphoma Diagnosis and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
Tohoku University
2016-2025
Toho University
2022
University of Yamanashi
2022
Shimizu (Japan)
2016
Kobe City Medical Center West Hospital
2016
Gunma University
2013-2014
Yamagata University
2001-2013
Ōtani University
2012
Duke Medical Center
2009-2010
Duke University Hospital
2010
Podoplanin (aggrus), a transmembrane sialoglycoprotein, is involved in tumor cell-induced platelet aggregation, metastasis, and lymphatic vessel formation. However, the mechanism by which podoplanin induces these cellular processes including its receptor has not been elucidated to date. induced aggregation with long lag phase, dependent upon Src phospholipase Cgamma2 activation. it does bind glycoprotein VI. This mode of activation was reminiscent snake toxin rhodocytin, identified us as...
Abstract Podoplanin (PDPN/Aggrus/T1α), a platelet aggregation-inducing mucin-like sialoglycoprotein, is highly expressed in many cancers and normal tissues. A neutralizing monoclonal antibody (mAb; NZ-1) can block the association between podoplanin C-type lectin-like receptor-2 (CLEC-2) inhibit podoplanin-induced cancer metastasis, but NZ-1 reacts with podoplanin-expressing cells such as lymphatic endothelial cells. In this study, we established cancer-specific mAb (CasMab) against human...
The mucin‐type sialoglycoprotein podoplanin (aggrus) is involved in tumor cell‐induced platelet aggregation and metastasis. C‐type lectin‐like receptor‐2 (CLEC‐2) was recently identified as an endogenous receptor of on platelets. However, the pathophysiological importance function CLEC‐2 have not been elucidated. Here we clarified interaction between vitro vivo . Using several deletion mutants expressed Fc chimeras, first important podoplanin‐recognition domain CLEC‐2. Furthermore,...
Platelets play an important role in hemostasis, thrombosis, and antimicrobial host defense are also involved the induction of inflammation, tissue repair, tumor metastasis. We have previously characterized platelet aggregation-inducing sialoglycoprotein (Aggrus/gp44) overexpressed on surface cells. Because a aggregation-neutralizing 8F11 monoclonal antibody that could specifically recognize Aggrus suppressed tumor-induced aggregation, we purified by 8F11-affinity chromatography found...
Aggrus (T1α/podoplanin, known as a specific marker for type I alveolar cells or lymphatic endothelial cells) is transmembrane sialoglycoprotein that aggregates platelets. Previously, we showed upregulated expression of occurs in colorectal tumors testicular and could be associated with platelet-aggregating activity metastatic ability. In tumors, specifically expressed seminoma. The present study investigates human primary lung cancer tissues different types. Microarray analysis demonstrated...
Abstract The taste receptor type 1 (T1r) family perceives ‘palatable’ tastes. These receptors function as T1r2-T1r3 and T1r1-T1r3 heterodimers to recognize a wide array of sweet umami (savory) tastes in sugars amino acids. Nonetheless, it is unclear how diverse are recognized by so few receptors. Here we present crystal structures the extracellular ligand-binding domains (LBDs), recognition regions fish heterodimer, bound different pocket T1r2LBD rich aromatic residues, spacious accommodates...
Glioblastoma (GBM) is the most common and lethal primary malignant brain tumor in adults with a 5-year overall survival rate of less than 10%. Podoplanin (PDPN) type I transmembrane mucin-like glycoprotein, expressed lymphatic endothelium. Several solid tumors overexpress PDPN, including mesenchymal GBM, which has been reported to present worst prognosis among GBM subtypes. Chimeric antigen receptor (CAR)-transduced T cells can recognize predefined surface antigens independent MHC...
CD44 is a transmembrane glycoprotein that regulates variety of genes related to cell-adhesion, migration, proliferation, differentiation, and survival. A large number alternative splicing isoforms CD44, containing various combinations exons, have been reported. standard (CD44s), which lacks variant widely expressed on the surface most tissues all hematopoietic cells. In contrast, show tissue-specific expression patterns extensively studied as both prognostic markers therapeutic targets in...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is among the cancers with poorest prognoses due to its highly malignant features. BTB and CNC homology 1 (BACH1) has been implicated in RAS-driven tumor formation. We focused on role of BACH1 PDAC, more than 90% which have KRAS mutation. Knockdown PDAC cell lines reduced migration invasion, part, by increasing E-cadherin expression, whereas overexpression showed opposite effects. directly repressed expression FOXA1 that known activate CDH1...
Epithelial cell adhesion molecule (EpCAM) is a type I transmembrane glycoprotein, which highly expressed on tumor cells. As EpCAM plays crucial role in adhesion, survival, proliferation, stemness, and tumorigenesis, it has been considered as promising target for diagnosis therapy. Anti‑EpCAM monoclonal antibodies (mAbs) have developed previously demonstrated outcomes several clinical trials. An anti‑EpCAM mAb, EpMab‑37 (mouse IgG<sub>1</sub>, kappa) was by the authors, using cell‑based...
CD44 is a cell surface glycoprotein, and its isoforms are produced by the alternative splicing with standard variant exons. The exon-containing (CD44v) overexpressed in carcinomas. CD44v6 one of CD44v, overexpression predicts poor prognosis colorectal cancer (CRC) patients. plays critical roles CRC adhesion, proliferation, stemness, invasiveness, chemoresistance. Therefore, promising target for diagnosis therapy CRC. In this study, we established anti-CD44 monoclonal antibodies (mAbs)...
Monoclonal antibody (mAb)-based and/or cell-based immunotherapies provide innovative approaches to cancer treatments. However, safety concerns over targeting normal cells expressing reactive antigens still exist. Therefore, the development of cancer-specific mAbs (CasMabs) that recognize with in vivo antitumor efficacy is required minimize adverse effects. We previously screened anti-human epidermal growth factor receptor 2 (HER2) and successfully established a anti-HER2 mAb, H
Overexpression of human epidermal growth factor receptor 2 (HER2) in breast and gastric cancers is an important target for monoclonal antibody (mAb) therapy. All therapeutic mAbs, including anti-HER2 exhibit adverse effects probably due to the recognition antigens expressed normal cells. Therefore, tumor-selective or specific mAbs can be beneficial reducing effects. In this study, we established a novel cancer-specific antibody, named H
Protein glycosylation is a critical subject attracting increasing attention in the field of proteomics as it expected to play key role investigation histological and diagnostic biomarkers. In this context, an enormous number glycoproteins have now been nominated disease-related However, there no appropriate strategy current proteome platform qualify such marker candidate molecules, which relates their specific expression particular diseases. Here, we present new practical system for focused...
Podoplanin (Aggrus) is a mucin‐type sialoglycoprotein that plays key role in tumor cell‐induced platelet aggregation. possesses aggregation‐stimulating (PLAG) domain, and Thr52 the PLAG domain of human podoplanin important for its activity. Endogenous or recombinant were purified, total glycosylation profiles surveyed by lectin microarray. Analyses glycopeptides produced Edman degradation mass spectrometry revealed disialyl‐corel (NeuAcα2‐3Galβl‐3(NeuAcα2‐6)GalNAcαl‐ O ‐Thr) structure was...