A5035350911- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Phytochemicals and Antioxidant Activities
- Agriculture, Plant Science, Crop Management
- Cancer Research and Treatments
- Plant Parasitism and Resistance
- Plant Diversity and Evolution
- Advanced biosensing and bioanalysis techniques
- Plant biochemistry and biosynthesis
- Plant Genetic and Mutation Studies
- RNA Interference and Gene Delivery
- Photosynthetic Processes and Mechanisms
- Plant and animal studies
- Wheat and Barley Genetics and Pathology
Kaneka (Japan)
2017-2024
Kaneka (United States)
2020-2024
AgriBio
2023
Takasago (Japan)
2017-2021
Tokyo University of Agriculture and Technology
2004-2006
The current application of genome editing to crop plants is limited cultivars that are amenable in vitro culture and regeneration. Here, we report an planta genome-editing which does not require callus Shoot apical meristems (SAMs) contain a subepidermal cell layer, L2, from germ cells later develop during floral organogenesis. biolistic delivery gold particles coated with plasmids expressing CRISPR/Cas9 components designed target TaGASR7 were bombarded into SAM-exposed embryos imbibed...
The currently favoured method for wheat (Triticum aestivum L.) transformation is inapplicable to many elite cultivars because it requires callus culture and regeneration. Here, we developed a simple, reproducible, in planta using biolistic DNA delivery without or Shoot apical meristems (SAMs) grown from dry imbibed seeds were exposed under microscope subjected bombardment with different-sized gold particles coated the GFP gene construct, introducing into L2 cell layer. Bombarded embryos...
Limitations for the application of genome editing technologies on elite wheat ( Triticum aestivum L.) varieties are mainly due to dependency in vitro culture and regeneration capabilities. Recently, we developed an planta particle bombardment (iPB) method which has increased process efficiency since no steps required create stably genome-edited plants. Here, report iPB commercially relevant Japanese varieties. The biolistic delivery gold particles coated with plasmids expressing CRISPR/Cas9...
Transformation is a key step in modern breeding technology that involves genome editing. The requirement for vitro tissue culture and regeneration hampers application of this to commercially important varieties many crop species. To overcome problem, we developed simple reproducible planta transformation method wheat (Tritticum aestivum L.). Our particle bombardment (iPB) utilizes the shoot apical meristem (SAM) as target tissue. SAM contains subepidermal cell layer termed L2, from which...
Here, we report a smart genome editing system for soybean (Glycine max) using the in planta bombardment-ribonucleoprotein (iPB-RNP) method without introducing foreign DNA or requiring traditional tissue culture processes such as embryogenesis and organogenesis. Shoot apical meristem (SAM) of embryonic axes was used target because SAM mature seeds has stem cells specific cell layers that develop germ during reproductive growth stage. In iPB-RNP method, RNP complex CRISPR/Cas9 directly...
Direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem via particle bombardment enabled introduction a semidwarf1-orthologous mutation an elite wheat variety.
Genome editing is highly useful for crop improvement. The method of expressing genome-editing enzymes using a transient expression system in
Abstract The CRISPR/Cas9 system has revolutionized the field of plant genetic engineering. Here we report a smart genome editing soybean by using iPB-RNP method without introducing foreign DNA and requiring traditional tissue culture processes such as embryogenesis organogenesis. Shoot apical meristem (SAM) embryonic axes was used target for editing, because SAM in mature seeds stem cells specific cell layer developing germ during reproductive growth stage. In method, complex...
A centrifugation-assisted peptide-mediated gene transfer (CAPT) method was recently developed as an efficient system for delivery into plant cells. However, the efficiency of CAPT cells not entirely satisfactory detecting transient expression a transgene driven mitochondria. Here, we report new using called particle bombardment-assisted (PBPT). We investigated various parameters PBPT to increase in Brassica campestris. The optimal conditions were single bombardment with gold particles coated...
Abstract In planta genome editing represents an attractive approach to engineering crops/varieties that are recalcitrant culture-based transformation methods. Here, we report the direct delivery of CRISPR/Cas9 ribonucleoproteins into shoot apical meristem using in particle bombardment and introduction a semidwarf1 (sd1) -orthologous mutation wheat. The triple knockout tasd1 mutant elite wheat variety reduced culm length by 10% without reduction yield.