A5007113313- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Molecular Junctions and Nanostructures
- Electrochemical Analysis and Applications
- Interactive and Immersive Displays
- Innovative Human-Technology Interaction
- Nanopore and Nanochannel Transport Studies
- Mechanical and Optical Resonators
- Antimicrobial Peptides and Activities
- Cellular Mechanics and Interactions
- Advanced biosensing and bioanalysis techniques
- Usability and User Interface Design
- Spectroscopy and Quantum Chemical Studies
- Tendon Structure and Treatment
- Microfluidic and Capillary Electrophoresis Applications
- Design Education and Practice
- Augmented Reality Applications
- Electrical and Thermal Properties of Materials
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Tactile and Sensory Interactions
- Proteoglycans and glycosaminoglycans research
- Collagen: Extraction and Characterization
- Heat Transfer and Boiling Studies
Istituto Nanoscienze
2016-2025
University of Modena and Reggio Emilia
2016-2025
Istituto Nazionale Biostrutture e Biosistemi
2022-2024
University of Urbino
2023
Umeå University
2022
National Research Council
2006-2022
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
2019
University of Dundee
2013-2018
Linnaeus University
2018
University of Warwick
2017
Here we review the applications of atomic force microscopy to study samples biological origin. Emphasis is given provide reader with information on broad range different biophysical that, date, such a technique can deal with. After recalling briefly operating principles an microscope, field bio-imaging faced (DNA, DNA–protein interaction, proteins, lipid membranes, cells); thereafter, use microscope measure forces introduced and mapping living cells discussed. This section followed by...
We are presenting a photography-derived methodology to achieve the photoreduction of Ag+−DNA complexes. λ-Phage DNA was first loaded with silver ions, then irradiated UV light at 254 nm. The bases acted as sensitizers, promoting in situ reduction Ag+ and formation metallic clusters. Three different approaches will illustrate this procedure, nanoparticle chains be grown along template rapid specific way.
Self-assembly of proteins, peptides, DNA, and other biomolecules to semiconductor quantum dots (QD) is an attractive bioconjugation route that can circumvent many the problems associated with covalent chemistry subsequent purification. Polyhistidine sequences have been shown facilitate self-assembly proteins peptides ZnS-overcoated CdSe QDs via complexation unoccupied coordination metal sites on nanocrystal surface. We describe synthesis characterization a thiol-reactive hexahistidine...
Human bone marrow-derived mesenchymal stem cells (hBMSCs) and their derivative enhanced green fluorescent protein (eGFP)-hBMSCs were employed to evaluate an innovative hybrid scaffold composed of granular hydroxylapatite collagen hemostat (Coll/HA). The cellular morphology/cytoskeleton organization cell viability investigated by immunohistochemistry (IHC) AlamarBlue metabolic assay, respectively. expression osteopontin osteocalcin proteins was analyzed IHC ELISA, whereas osteogenic genes...
Metalloproteins are redox molecules naturally shuttling electrons with high efficiency between molecular partners. As such, they candidates of choice for bioelectronics. In this work, we have used bacterial metalloprotein azurin, hosted in a nanometer gap two electrically biased gold electrodes, to demonstrate an electrochemically gated single-molecule transistor operating aqueous environment. Gold-chemisorbed azurin shows peaks tunneling current upon changing electrode potential and related...
The usual biophysical approach to the study of biological membranes is that turning model systems. From these models, general physical principles ruling lateral membrane structure can be obtained. A promising system supported lipid bilayer (SLB) which could foresee simultaneous investigation and properties bilayers reconstituted with proteins. complete exploitation retrieve biologically relevant information requires an in-depth knowledge possible effect experimental parameters have on...
Fundamental knowledge about the mechanisms of adhesion between oxide particles with diameters few nanometers is impeded by difficulties associated direct measurements contact forces at such a small size scale. Here we develop strategy based on AFM force spectroscopy combined all-atom molecular dynamics simulations to quantify and explain nature 10 nm TiO(2) nanoparticles. The method statistical analysis peaks measured in repeated approaching/retracting loops an cantilever into film...
Aberrant splicing events are associated with colorectal cancer (CRC) and provide new opportunities for tumor diagnosis treatment. The expression of the splice variants NF-YA, DNA binding subunit transcription factor NF–Y, is deregulated in multiple types compared to healthy tissues. NF–YAs NF–YAl isoforms differ transactivation domain, which may result distinct transcriptional programs. In this study, we demonstrated that transcript higher aggressive mesenchymal CRCs predicts shorter...
Glioblastoma multiforme (grade IV glioma) is characterized by a high invasive potential making surgical intervention extremely challenging and patient survival very limited. Current pharmacological approaches show at best slight improvements in the therapy against this type of tumour. Microtubules are often target antitumoral drugs, specific drugs affecting their dynamics acting on microtubule associated proteins (MAPs) without producing depolymerization could affect both glioma cell...
Glioblastoma multiforme (grade IV glioma) is characterized by a high invasive potential, making surgical intervention extremely challenging and patient survival very limited. Current pharmacological approaches show, at best, slight improvements in the therapy against this type of tumor. Microtubules are often target antitumoral drugs, specific drugs affecting their dynamics acting on microtubule-associated proteins (MAPs) without producing depolymerization could affect both glioma cell...
An integrated field-effect device for fully electronic deoxyribonucleic acid (DNA) detection was realized in a standard CMOS process. The is composed of floating-gate MOS transistor, control-capacitor acting as counterelectrode, and an exposed active area DNA immobilization. drain-current the transistor modulated by electric charge carried molecules. After hybridization, this increases change output current measured. Experimental results are provided. Full compatibility with process opens...
Among perinatal stem cells of the umbilical cord, human Wharton's jelly mesenchymal (hWJ-MSCs) are great interest for cell-based therapy approaches in regenerative medicine, showing some advantages over other MSCs. In fact, hWJ-MSCs, placed between embryonic and adult MSCs, not tumorigenic harvested with few ethical concerns. Furthermore, these can be easily cultured vitro, maintaining both properties a high proliferative rate several passages, as well trilineage capacity differentiation....