A5089644002- Force Microscopy Techniques and Applications
- Molecular Junctions and Nanostructures
- Lipid Membrane Structure and Behavior
- Electrochemical Analysis and Applications
- Photosynthetic Processes and Mechanisms
- Quantum Dots Synthesis And Properties
- Spectroscopy and Quantum Chemical Studies
- Chalcogenide Semiconductor Thin Films
- Mechanical and Optical Resonators
- Advanced biosensing and bioanalysis techniques
- Nanopore and Nanochannel Transport Studies
- Photoreceptor and optogenetics research
- Electrochemical sensors and biosensors
- Acoustic Wave Resonator Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Integrated Circuits and Semiconductor Failure Analysis
- Ion channel regulation and function
- Quantum and electron transport phenomena
- RNA Interference and Gene Delivery
- Copper-based nanomaterials and applications
- Microfluidic and Bio-sensing Technologies
- Neuroscience and Neural Engineering
- Near-Field Optical Microscopy
- Surface Chemistry and Catalysis
- Surfactants and Colloidal Systems
Instituto di Biofisica
1997-2022
National Research Council
2006-2021
University of Modena and Reggio Emilia
2003-2019
Istituto Nanoscienze
2010-2019
National Academies of Sciences, Engineering, and Medicine
2007-2015
University of Milan
2008
Institute of Biophysics
2008
Tel Aviv University
2007
Russian Academy of Sciences
2007
Institute of Bioorganic Chemistry
2007
Here we review the applications of atomic force microscopy to study samples biological origin. Emphasis is given provide reader with information on broad range different biophysical that, date, such a technique can deal with. After recalling briefly operating principles an microscope, field bio-imaging faced (DNA, DNA–protein interaction, proteins, lipid membranes, cells); thereafter, use microscope measure forces introduced and mapping living cells discussed. This section followed by...
We are presenting a photography-derived methodology to achieve the photoreduction of Ag+−DNA complexes. λ-Phage DNA was first loaded with silver ions, then irradiated UV light at 254 nm. The bases acted as sensitizers, promoting in situ reduction Ag+ and formation metallic clusters. Three different approaches will illustrate this procedure, nanoparticle chains be grown along template rapid specific way.
Self-assembly of proteins, peptides, DNA, and other biomolecules to semiconductor quantum dots (QD) is an attractive bioconjugation route that can circumvent many the problems associated with covalent chemistry subsequent purification. Polyhistidine sequences have been shown facilitate self-assembly proteins peptides ZnS-overcoated CdSe QDs via complexation unoccupied coordination metal sites on nanocrystal surface. We describe synthesis characterization a thiol-reactive hexahistidine...
Metalloproteins are redox molecules naturally shuttling electrons with high efficiency between molecular partners. As such, they candidates of choice for bioelectronics. In this work, we have used bacterial metalloprotein azurin, hosted in a nanometer gap two electrically biased gold electrodes, to demonstrate an electrochemically gated single-molecule transistor operating aqueous environment. Gold-chemisorbed azurin shows peaks tunneling current upon changing electrode potential and related...
The usual biophysical approach to the study of biological membranes is that turning model systems. From these models, general physical principles ruling lateral membrane structure can be obtained. A promising system supported lipid bilayer (SLB) which could foresee simultaneous investigation and properties bilayers reconstituted with proteins. complete exploitation retrieve biologically relevant information requires an in-depth knowledge possible effect experimental parameters have on...
Fundamental knowledge about the mechanisms of adhesion between oxide particles with diameters few nanometers is impeded by difficulties associated direct measurements contact forces at such a small size scale. Here we develop strategy based on AFM force spectroscopy combined all-atom molecular dynamics simulations to quantify and explain nature 10 nm TiO(2) nanoparticles. The method statistical analysis peaks measured in repeated approaching/retracting loops an cantilever into film...
We show that the electron-transfer protein azurin can be used to fabricate biomolecular rectifiers exploiting its native redox properties, chemisorption capability and electrostatic features. The devices consist of a layer interconnecting nanoscale electrodes fabricated by electron beam lithography. They exhibit rectification ratio as large 500 at 10 V, operate room temperature in air.
A protein-based solid-state electronic device that operates in air is reported; it fabricated by interconnecting an azurin monolayer immobilized on SiO2 with two gold nanoelectrodes. The operating mechanism ascribed to resonant tunneling through the redox active center, combined macroscopic polarization of oriented self-organized protein layer. Figure shows molecular electrostatic potential oxidized protein.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation of Ultrathin Semiconductor Films by CdS Nanostructure AggregationPaolo Facci, Victor Erokhin, Andrei Tronin, and Claudio NicoliniCite this: J. Phys. Chem. 1994, 98, 50, 13323–13327Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://pubs.acs.org/doi/10.1021/j100101a036https://doi.org/10.1021/j100101a036research-articleACS PublicationsRequest reuse...
An integrated field-effect device for fully electronic deoxyribonucleic acid (DNA) detection was realized in a standard CMOS process. The is composed of floating-gate MOS transistor, control-capacitor acting as counterelectrode, and an exposed active area DNA immobilization. drain-current the transistor modulated by electric charge carried molecules. After hybridization, this increases change output current measured. Experimental results are provided. Full compatibility with process opens...
Cyclic voltammetry experiments were carried out on native Saccharomyces cerevisiae iso-1-cytochrome c and its C102T/N62C variant immobilized bare polycrystalline gold electrode through the S−Au bond formed by a surface cysteine. Experiments at different temperatures (5−65 °C) pH values (1.5−7). The E°' value 7 (+370 mV vs SHE) is approximately 100 higher than that for protein in solution. This difference enthalpic origin proposed to be result of electrostatic repulsion among densely packed...
Nowadays, there is much experimental evidence that the mechanical properties of biological membranes affect membrane protein functions. A very convenient technique to study these on a spatial scale relevant single proteins represented by Atomic Force Spectroscopy (AFS). In this we measured force AFM tip has apply supported lipid bilayer punch-through it as function different environmental parameters. We observed reduced when in its phase transition region. interpreted our results basis...