A5050951483- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Photosynthetic Processes and Mechanisms
- Conducting polymers and applications
- Analytical Chemistry and Sensors
- Molecular Junctions and Nanostructures
- Advanced biosensing and bioanalysis techniques
- Hemoglobin structure and function
- Neuroscience and Neural Engineering
- Advanced Memory and Neural Computing
- Photoreceptor and optogenetics research
- Spectroscopy and Quantum Chemical Studies
- Organic Electronics and Photovoltaics
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Force Microscopy Techniques and Applications
- Mitochondrial Function and Pathology
- Enzyme-mediated dye degradation
- Alzheimer's disease research and treatments
- Nanowire Synthesis and Applications
- Cardiomyopathy and Myosin Studies
- Erythrocyte Function and Pathophysiology
- Cellular transport and secretion
- Enzyme Structure and Function
- Extracellular vesicles in disease
University of Modena and Reggio Emilia
2016-2025
Azienda Unita' Sanitaria Locale Di Modena
2016-2020
Istituto Nanoscienze
2012-2017
National Research Council
2016
Center for NanoScience
2016
Consorzio Roma Ricerche
2016
In-Q-Tel
2013
University of Milan
2008
Institute of Bioorganic Chemistry
2007
Russian Academy of Sciences
2007
Electrolyte-gated transistors (EGTs), capable of transducing biological and biochemical inputs into amplified electronic signals stably operating in aqueous environments, have emerged as fundamental building blocks bioelectronics. In this Primer, the different EGT architectures are described with mechanisms underpinning their functional operation, providing insight key experiments including necessary data analysis validation. Several organic inorganic materials used structures fabrication...
Antibody-antigen (Ab-Ag) recognition is the primary event at basis of many biosensing platforms. In label-free biosensors, these events occurring solid-liquid interfaces are complex and often difficult to control technologically across smallest length scales down molecular scale. Here a molecular-scale technique, such as single-molecule force spectroscopy, performed areas real electrode functionalized for immunodetection an inflammatory cytokine, viz. interleukin-4 (IL4). The statistical...
Lytic polysaccharide monooxygenases (LPMOs) are Cu-containing enzymes that facilitate the degradation of recalcitrant polysaccharides by oxidative cleavage glycosidic bonds. They gaining rapidly increasing attention as key players in biomass conversion, especially for production second-generation biofuels. Elucidation detailed mechanism LPMO reaction is a major step toward assessment and optimization efficacy industrial biotechnology, paving way to utilization sustainable fuel sources. Here,...
Abstract Organic electronic transistors are rapidly emerging as ultrahigh sensitive label‐free biosensors suited for point‐of‐care or in‐field deployed applications. Most organic reported to date based on immunorecognition between the relevant biomarkers and immobilized antibodies, whose use is hindered by large dimensions, poor control of sequence, relative instability. Here, an electrolyte‐gated field effect transistor (EGOFET) biosensor where recognition units surface peptide aptamers...
The bacterium Shewanella oneidensis has evolved a sophisticated electron transfer (ET) machinery to export electrons from the cytosol extracellular space during respiration. At heart of this process are decaheme proteins Mtr pathway, MtrC and MtrF, located at external face outer bacterial membrane. Crystal structures have revealed that these bind 10 c-type hemes arranged in peculiar shape staggered cross trifurcates flow, presumably reduce substrates while directing neighboring multiheme...
Abstract Low‐cost, minimally invasive sensors able to provide real‐time monitoring of wound infection can enable the optimization healthcare resources in chronic wounds management. Here, a novel printed organic electrochemical transistors (OECT) biosensor for uric acid (UA), bacterial biomarker wounds, is demonstrated artificial exudate. The sensor exploits enzymatic conversion UA 5‐hydroxyisourate, catalyzed by Uricase entrapped dual‐ionic‐layer hydrogel membrane casted onto gate. response...
Biorecognition is a central event in biological processes the living systems that also widely exploited technological and health applications. We demonstrate Electrolyte Gated Organic Field Effect Transistor (EGOFET) an ultrasensitive specific device allows us to quantitatively assess thermodynamics of biomolecular recognition between human antibody its antigen, namely, inflammatory cytokine TNFα at solid/liquid interface. The EGOFET biosensor exhibits superexponential response concentration...
Dynamic protein–solvent interactions are fundamental for life processes, but their investigation is still experimentally very demanding. Molecular dynamics simulations up to hundreds of nanoseconds can bring light unexpected events even extensively studied biomolecules. This paper reports a combined computational/experimental approach that reveals the reversible opening two distinct fluctuating cavities in Saccharomyces cerevisiae iso-1-cytochrome c. Both channels allow water access heme...
Significance How well a protein conducts electrical current depends on both the chemical nature of and its contacts to electrodes between which currents are carried. Investigating conduction via monolayers, we find that covalent binding doubles room temperature halves thermal activation energy. At low temperatures, where transport is by tunneling, increases up 10-fold. To examine across protein, used seven different cytochrome c mutants with surface-exposed cysteine, providing distinct...
The quantification of urea is the utmost importance not only in medical diagnosis, where it serves as a potential indicator kidney and liver disfunction, but also food safety environme ...
Cytokines are small proteins that play fundamental roles in inflammatory processes the human body. In particular, interleukin (IL)-6 is a multifunctional cytokine, whose increased levels associated with infection, cancer, and inflammation. The quantification of IL-6 therefore primary importance early stages inflammation chronic diseases, but standard techniques expensive, time-consuming, usually rely on fluorescent or radioactive labels. Organic electronic devices and, organic field-effect...
Electrolyte gated organic transistors can operate as powerful ultrasensitive biosensors, and efforts are currently devoted to devising strategies for reducing the contribution of hardly avoidable, nonspecific interactions their response, ultimately harness selectivity in detection process. We report a novel lab-on-a-chip device integrating multigate electrolyte field-effect transistor (EGOFET) with 6.5 μL microfluidics set up capable provide an assessment both response reproducibility, by...
Elucidation of the molecular determinants reorganization energy λ is central to understanding fundamental biological processes based on transduction pathways. Here, we use a combined experimental/theoretical approach electrochemically determine for number cytochrome c variants and compute structure-related properties relevant kinetics electron transfer process through dynamics simulations. We find that exposure heme group solvent controls investigated proteins. Therefore, fine-tuning can be...
In this work, we demonstrate the ultrasensitive and selective detection of dopamine by means a neuro-inspired device platform without need specific recognition moiety. The sensor is whole organic featuring two electrodes made poly(3,4-ethylenedioxythiophene):polystyrenesulfonate-PEDOT:PSS-patterned on polydymethylsiloxane-PDMS-flexible substrate. One electrode pulsed with train voltage square waves, to mimic presynaptic neuron behavior, while other used record displacement current, mimicking...
Congenital myopathies are typically characterised by early onset hypotonia, weakness and hallmark features on biopsy. Despite the rapid pace of gene discovery, ∼50% patients with a congenital myopathy remain without genetic diagnosis following screening known disease genes. We performed exome sequencing two consanguineous probands diagnosed muscle biopsy showing selective atrophy/hypotrophy or absence type II myofibres. identified variants in (MYL1) encoding skeletal fast-twitch specific...
Abstract Organic electrochemical transistors (OECTs) are finding widespread application in biosensing, thanks to their high sensitivity, broad dynamic range, and low limit of detection. An OECT biosensor requires the immobilization a biorecognition probe on gate, or else channel, through several, often lengthy, chemical steps. In this work, fast straightforward way functionalize carbon gate fully screen‐printed by means polydopamine (PDA) film is presented. By an amine‐terminated...
The advent of immunotherapies with biological drugs has revolutionized the treatment cancers and auto-immune diseases. However, in some patients, production anti-drug antibodies (ADAs) hampers drug efficacy. concentration ADAs is typically range 1-10 pm; hence their immunodetection challenging. toward Infliximab (IFX), a used to treat rheumatoid arthritis other diseases, are focussed. An ambipolar electrolyte-gated transistor (EGT) immunosensor reported based on reduced graphene oxide (rGO)...
Thermodynamic and dynamic properties of iso-1-cytochrome c covalently bound to a bare gold surface are here investigated by large scale atomistic simulations. The reduction potential the protein for low high concentrations is calculated showing good agreement with experimental estimates. origin dependence on concentration demonstrated stem from changing polarizability environment surrounding protein, mechanism reminiscent crowding effects. Moreover, structural analyses performed revealing...