A5000500660- Bacterial Identification and Susceptibility Testing
- Diverticular Disease and Complications
- Leptospirosis research and findings
- Peroxisome Proliferator-Activated Receptors
- Infections and bacterial resistance
- Infectious Disease Case Reports and Treatments
- Streptococcal Infections and Treatments
- Adipose Tissue and Metabolism
- Antimicrobial Resistance in Staphylococcus
- Viral Infections and Vectors
- Actinomycetales infections and treatment
- Veterinary medicine and infectious diseases
- Mycobacterium research and diagnosis
- Viral gastroenteritis research and epidemiology
- Biliary and Gastrointestinal Fistulas
- Animal Virus Infections Studies
- Antibiotic Resistance in Bacteria
- Vector-borne infectious diseases
- Microbial infections and disease research
- Metabolism and Genetic Disorders
- Reproductive tract infections research
- Viral Infections and Immunology Research
- Helminth infection and control
- Infectious Diseases and Mycology
- Salmonella and Campylobacter epidemiology
University of Kentucky
2014-2025
Centers for Disease Control and Prevention
2013
Texas A&M University
2003-2011
University of Nebraska–Lincoln
2011
Tall Timbers Research Station and Land Conservancy
2011
Wellborn Road Veterinary Medical Center
2006-2007
University of Missouri
1999
Although liver fatty acid-binding protein (L-FABP) is an important binding site for various hydrophobic ligands in hepatocytes, its vivo significance not understood. We have therefore created L-FABP null mice and report here their initial analysis, focusing on the impact of this mutation hepatic acid capacity, lipid composition, expression other lipid-binding proteins. Gel-filtered cytosol from lacked main peak fraction that normally comprises both sterol carrier protein-2 (SCP-2). The...
The goal of this retrospective study was to have a comprehensive picture the β-hemolytic streptococci horses including tissue/organ distributions and susceptibility patterns against specific antimicrobials between January 1, 2000 December 31, 2010. A total 2,497 were isolated from 2,391 cases, which Streptococcus equi subsp. zooepidemicus most frequent isolate (72.0%). Other species dysgalactia equisimilis (21.3%), (5.8%), unidentified (0.9%). As expected, S. mostly lymph node abscesses...
Liver fatty acid binding protein (L-FABP) has been proposed to limit the availability of long-chain acids (LCFA) for oxidation and peroxisome proliferator-activated receptor alpha (PPAR-alpha), a transcription factor that determines capacity hepatic oxidation. Here, we used L-FABP null mice test this hypothesis. Under fasting conditions, mutation reduced beta-hydroxybutyrate (BHB) plasma levels as well BHB release palmitic by isolated hepatocytes. However, ketogenesis was not reduced: were...
The objective of this study was to determine the prevalence Rhodococcus equi strains resistant macrolides and rifampin over time in clinical samples from foals submitted diagnostic laboratories central Kentucky. We performed a retrospective observational all that were veterinary Kentucky between January 1995 December 2017. Samples included if R. bacterium cultured tested for vitro susceptibility erythromycin or rifampin. In testing available 2,169 isolates equi, while both 1,681 isolates....
ABSTRACT Salmonella is a foodborne pathogen that poses significant threat to global public health. It affects several animal species, including horses. infections in horses can be either asymptomatic or cause severe clinical illness. Infections caused by are presently controlled with antibiotics. Due the formation of biofilms and emergence antimicrobial resistance, treatment has become more complicated. Our study focused on investigating prevalence enterica necropsied horses, assessing...
The aim of this study was to determine the MICs 32 antimicrobial agents for 200 isolates Rhodococcus equi animal origin by applying a recently described broth microdilution protocol, and investigate with distinctly elevated rifampicin genetic basis resistance. included R. isolates, including 160 from horses 40 other sources, USA Europe. MIC testing or combinations thereof followed published protocol. A novel PCR protocol joint amplification three rpoB regions in which resistance-mediating...
Resistance to commonly used antimicrobials is a growing concern in both human and veterinary medicine. Understanding the temporal changes burden of problem identifying its determinants important for guiding control efforts. Therefore, objective this study was investigate patterns predictors antimicrobial resistance among Staphylococcus spp. isolated from canine specimens submitted University Kentucky Veterinary Diagnostic Laboratory (UKVDL) between 1993 2009.Retrospective data 4,972 isolates...
A 7-year-old pregnant Angus cow was found dead in the field. At necropsy, aortic valve expanded by moderate fibrous connective tissue and acidophilic coagulum containing multifocal marked bacteria, mineral, neutrophils, red blood cells. Numerous tiny grayish, opaque bacterial colonies were detected on agar plates at 7 days after inoculation with a swab of heart cow. The bacterium Gram-negative, very small coccobacillus that catalase, oxidase, urease negative, did not change litmus milk,...
Equine rotavirus group A (ERVA) is one of the most common causes foal diarrhea. Starting in February 2021, there was an increase frequency severe watery to hemorrhagic diarrhea cases neonatal foals Central Kentucky. Diagnostic investigation fecal samples failed detect evidence diarrhea-causing pathogens including ERVA. Based on
A comprehensive evaluation of the real-time PCR assay for leptospirosis in comparison with other diagnostic assays on a large-scale basis is fundamental validating and determining causes equine abortions.To compare evaluate value traditional methods leptospiral abortions.Cross-sectional observational study.A Leptospira spp. fluorescent antibody test (FAT), microscopic agglutination (MAT) (targeting LipL32 gene) were compared evaluated fetal necropsy specimens (placenta, kidney, liver heart...
The low-molecular-mass, cytosolic heart-type fatty acid-binding protein (H-FABP) is thought to be required for shuttling FA through the cytosol. Therefore, we examined effects of an H-FABP-null mutation on and carbohydrate metabolism in isolated soleus muscle at rest during a period increased metabolic demand (30-min contraction). There were lower concentrations creatine phosphate (-41%), ATP (-22%), glycogen (-34%), lactate (-31%) ( P < 0.05) muscles, but no differences citrate synthase...
The pathogenic role of beta-hemolytic Streptococcus dysgalactiae in the equine host is increasingly recognized. A collection 108 Lancefield group C (n = 96) or L 12) horse isolates recovered United States and three European countries presented multilocus sequence typing (MLST) alleles, types emm (only 56% could be typed) that were, with few exceptions, distinct from those previously found human subsp. equisimilis. Characterization a subset by analysis (MLSA) 16S rRNA gene showed most also...
Summary Reasons for performing study Nocardioform placentitis in horses is poorly understood, and the development of an experimental model would be help understanding pathogenesis disease. Objectives To investigate whether (1) intrauterine inoculation C rossiela equi during periovulatory period or (2) i.v., oral intranasopharyngeal . midgestation result nocardioform placentitis, (3) before after mating endometrial swabs present evidence placentitis‐associated organisms ( A mycolatopsis...
Currently available diagnostic assays for leptospirosis cannot differentiate vaccine from infection serum antibody. Several leptospiral proteins that are upregulated during have been described, but their utility as a marker is still unclear. In this study, we undertook lipidomics approach to determine if there any differences in the lipid profiles of horses naturally infected with pathogenic Leptospira spp. and vaccinated against commercially bacterin. Utilizing high-resolution mass...
Antimicrobial resistance limits traditional treatment options and increases costs. It is therefore important to estimate the magnitude of problem so as provide empirical data guide control efforts. The aim this study was investigate burden patterns antimicrobial (AMR) among equine Staphylococcus samples submitted University Kentucky Veterinary Diagnostic Laboratory (UKVDL) from 1993 2009. Retrospective 1711 UKVDL during time period 2009 were included in study. susceptibility testing, that 16...
Heart-type fatty acid-binding protein (H-FABP) is required for high rates of skeletal muscle long-chain acid (LCFA) oxidation and esterification. Here we assessed whether H-FABP affects soleus glucose uptake when measured in vitro the absence LCFA. Wild-type null mice were fed a standard chow or high-fat diet before isolation. With chow, mutation increased insulin-dependent deoxyglucose by 141% (P < 0.01) at 0.02 mU/ml insulin but did not cause significant effect 2 insulin; triglyceride...
Abstract Objective —To estimate the sensitivity (Se) and specificity (Sp) for an enhanced direct-fecal PCR procedure, bacterial culture of feces (BCF), a serum ELISA detecting Mycobacterium avium subsp paratuberculosis (MAP) infection in adult dairy cattle. Sample Population —Fecal samples were collected from 669 cattle randomly selected 4,000-cow herd known to contain animals infected with MAP. Procedures —Serum evaluated MAP-specific antibodies via ELISA. Fecal by BCF methods (both...
Nocardioform actinomycetes are significant causes of placentitis and abortions in horses. In the current study, antimicrobial susceptibility patterns 38 Amycolatopsis spp. 22 Crossiella equi isolates, most common nocardioform causing horses, were evaluated. Antimicrobial susceptibilities these isolates tested by broth microdilution method a commercial system, which was designed for Nocardia spp., fast-growing Mycobacterium other aerobic actinomycetes. The minimum inhibitory concentration...
Clonal multidrug resistance recently emerged in Rhodococcus equi, complicating the therapeutic management of this difficult-to-treat animal- and human-pathogenic actinomycete. The currently spreading multidrug-resistant (MDR) "2287" clone arose equine farms upon acquisition, coselection by mass macrolide-rifampin therapy, pRErm46 plasmid carrying erm(46) macrolide-lincosamide-streptogramin determinant, an rpoBS531F mutation. Here, we screened a collection susceptible macrolide-resistant R....