A5029090461- Iron Metabolism and Disorders
- Escherichia coli research studies
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Clostridium difficile and Clostridium perfringens research
- Cystic Fibrosis Research Advances
- HIV/AIDS drug development and treatment
- Tannin, Tannase and Anticancer Activities
- Mycobacterium research and diagnosis
- HIV/AIDS Research and Interventions
- Enzyme Production and Characterization
- Crystallization and Solubility Studies
- Polymer Synthesis and Characterization
- Heme Oxygenase-1 and Carbon Monoxide
- Bacteriophages and microbial interactions
- Biofuel production and bioconversion
- Microwave-Assisted Synthesis and Applications
- Trace Elements in Health
- Porphyrin Metabolism and Disorders
- HIV Research and Treatment
- Drug Transport and Resistance Mechanisms
- Probiotics and Fermented Foods
Oklahoma State University
2021-2025
Oklahoma State University Oklahoma City
2023
University of Alabama at Birmingham
2015-2022
University of South Florida
2011-2014
Iron is essential for replication of Mycobacterium tuberculosis, but iron efficiently sequestered in the human host during infection. Heme constitutes largest reservoir body and utilized by many bacterial pathogens as an source. While heme acquisition well studied other pathogens, little known M. tuberculosis To identify proteins involved utilization a transposon mutant library was screened resistance to toxic analog gallium(III)-porphyrin (Ga-PIX). Inactivation ppe36, ppe62, rv0265c genes...
Iron is essential for growth of Mycobacterium tuberculosis (Mtb), but most iron in the human body stored heme within hemoglobin. Here, we demonstrate that substrate-binding protein DppA inner membrane Dpp transporter required and hemoglobin utilization by Mtb. The 1.27 Å crystal structure shows a tetrapeptide bound core large solvent-exposed crevice binding. Mutation arginine 179 this cleft eliminates binding to prevents outer proteins PPE36 PPE62 are also utilization, indicating these...
The equine gut harbours a diverse microbial community and represents rich source of carbohydrate-active enzymes (CAZymes). To identify characterize potentially novel CAZymes from horse's hindgut metagenome, shotgun metagenomic sequencing was performed on DNA extracted stool sample male horse followed by CAZyme annotation. Here, we report the characterization enzyme (AH2) that identified, synthesized, cloned characterized obtained dataset. AH2 identified as GH130 family member displayed an...
M. tuberculosis ( Mtb ) is completely dependent on iron acquisition in the host to cause disease. The largest source of for human heme.
Mycobacterium tuberculosis infects ~25% of the world’s population and causes (TB), second leading cause death from infectious disease. Heme is an essential metabolite for M. , targeting unique heme biosynthetic pathway could serve as effective therapeutic strategy.
Mycobacterium tuberculosis, the causative agent of human tuberculosis (TB), has surpassed HIV/AIDS as leading cause death from a single infectious agent. The increasing occurrence drug-resistant strains become major challenge for health care systems and, in some cases, rendered TB untreatable. However, development new drugs been plagued with high failure rates and costs. Alternative strategies to increase efficacy current treatment regimens include host-directed therapies or agents that make...
Enterohemorrhagic Escherichia coli (EHEC) is dependent on acid resistance for gastric passage and low oral infectious dose, the locus of enterocyte effacement (LEE) intestinal colonization. Mutation rpoN, encoding sigma factor N (σ(N)), dramatically alters growth-phase regulation both LEE. This study reports determinants σ(N)-directed LEE expression, underlying mechanism attributable to this phenotype. Glutamate-dependent (GDAR) in TW14359ΔrpoN correlated with increased expression gadX-gadW...
Abstract In enterohemorrhagic Escherichia coli ( EHEC ), sigma factor N σ ) regulates glutamate‐dependent acid resistance GDAR and the locus of enterocyte effacement LEE ); discrete genetic systems that are required for transmission virulence this intestinal pathogen. Regulation these requires nitrogen regulatory protein C, NtrC, is a consequence NtrC‐ ‐dependent reduction in activity S ). This study elucidates pathway components stimuli ‐directed regulation . Deletion fliZ , product which...
Since acquiring iron is paramount to P. aeruginosa ’s survival and colonization in the human host, developing novel strategies block access of host will allow us starve it an essential nutrient. uses siderophore, heme, or ferrous uptake systems acquire host. We have developed a approach through which we can directly identify molecules that prevent from utilizing heme iron.
Heme is both an essential cofactor and abundant source of nutritional iron for the human pathogen Mycobacterium tuberculosis (Mtb). While heme required Mtb survival virulence, it also potentially cytotoxic. Since has ability to make uptake heme, de novo synthesis its acquisition from host must be balanced in order mitigate toxicity. However, mechanisms employed by regulate uptake, synthesis, bioavailability are poorly understood. By integrating ratiometric sensors with mycobacterial...