A5080799523- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Heat shock proteins research
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Endoplasmic Reticulum Stress and Disease
- Exercise and Physiological Responses
- Antimicrobial Peptides and Activities
- thermodynamics and calorimetric analyses
- Dermatology and Skin Diseases
- Toxin Mechanisms and Immunotoxins
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Allergic Rhinitis and Sensitization
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- Pharmacological Effects of Natural Compounds
- Leptospirosis research and findings
- Bee Products Chemical Analysis
- Rheumatoid Arthritis Research and Therapies
- Hepatitis B Virus Studies
- Immune cells in cancer
- Vector-borne infectious diseases
- Autoimmune and Inflammatory Disorders Research
- Lipid Membrane Structure and Behavior
Utrecht University
2016-2025
University of Veterinary Medicine
2015
Amsterdam UMC Location Vrije Universiteit Amsterdam
2003-2009
Vrije Universiteit Amsterdam
2003
Erasmus University Rotterdam
1985
Immune responses to heat shock proteins (Hsp) develop in virtually all inflammatory diseases; however, the significance of such is only now becoming clear. In experimental disease models, Hsp administration can prevent or arrest damage, and initial clinical trials patients with chronic diseases, peptides have been shown promote production anti-inflammatory cytokines, indicating immunoregulatory potential Hsp. Therefore, presence immune diseases be seen as an attempt system correct condition....
Reestablishing self-tolerance in autoimmunity is thought to depend on self-reactive regulatory T cells (Tregs). Exploiting these antigen-specific regulators hampered by the obscure nature of disease-relevant autoantigens. We have uncovered potent disease-suppressive Tregs recognizing Heat Shock Protein (Hsp) 70 self-antigens, enabling selective activity inflamed tissues. Hsp70 a major contributor MHC class II ligandome. Here we show that conserved epitope (B29) present murine and upon...
Until recently, the immune system was seen solely as a defense with its primary task being elimination of unwanted microbial invaders. Currently, however, functional significance has obtained much wider perspective, to include among others maintenance and restoration homeostasis following tissue damage. In this latter aspect, there is growing interest in identification molecules involved, such so-called danger or damage-associated molecular patterns (DAMPs), also called alarmins. Since heat...
Stress proteins, such as members of the heat-shock protein (HSP) family, are up-regulated by cells in inflamed tissue and can be viewed functionally "biomarkers" for immune system to monitor inflammation. Exogenous administration stress proteins has induced immunoregulation various models inflammation also been shown effective clinical trials humans. This study was undertaken test hypothesis that boosting endogenous HSP expression restore through T specific proteins.Stress manipulated vivo...
Development of vaccines in autoimmune diseases has received wide attention over the last decade. However, many showed limited clinical efficacy. To enhance vaccine efficacy infectious diseases, biocompatible and biodegradable polymeric nanoparticles have gained interest as antigen delivery systems. We investigated mice whether antigen-encapsulated PLGA (poly-lactic-co-glycolic acid), PLGA-TMC (N-trimethyl chitosan) or TMC-TPP (tri-polyphosphate) can also be used to modulate immunological...
Canine atopic dermatitis is a genetically predisposed inflammatory and pruritic allergic skin disease that often complicated by (secondary) bacterial fungal (yeast) infections. High-throughput DNA sequencing was used to characterize the composition of microbiome (bacteria fungi) inhabiting specific sites in healthy dogs with (AD) before after topical antimicrobial treatment. Skin samples were collected from six control three spontaneously affected AD swabbing at (non-) predilection before,...
Oral antigen application induces tolerance, leading to suppression of a subsequent systemic challenge with this antigen. The is mediated by mucosal regulatory T (Tr) cells that may differentiate from naive peripheral in the gut-draining lymphoid tissue. However, little known about initial steps differentiation process. In study we show 48 h after oral OVA treatment, antigen-specific mesenteric lymph nodes (MLN) and Peyer's Patches (PP) were activated had divided up four times. first division...
The skin is an attractive organ for immunization due to the presence of a large number epidermal and dermal antigen-presenting cells. Hollow microneedles allow precise non-invasive intradermal delivery vaccines. In this study, ovalbumin (OVA)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles with without TLR3 agonist poly(I:C) were prepared administered intradermally by hollow microneedles. capacity PLGA induce cytotoxic T cell response, contributing protection against intracellular...
Previous studies in mouse models of autoimmune diabetes and encephalomyelitis have indicated that the selective delivery self-antigen to endocytic receptor DEC205 on steady-state dendritic cells (DCs) may represent a suitable approach induce Ag-specific immune tolerance. In this study, we aimed examine whether DEC205(+) DC targeting single immunodominant peptide derived from human cartilage proteoglycan (PG) can promote tolerance PG-induced arthritis (PGIA). Besides disease induction by...
The pathogenesis of many inflammatory diseases is associated with the uncontrolled activation nuclear factor kappa B (NF-κB) in macrophages. Previous studies have shown that various cell types, heat shock protein 70 (Hsp70) plays a crucial role controlling NF-κB activity. So far, little known about Hsp70 canine processes. In this study we investigated potential anti-inflammatory effects macrophages as well mechanisms underlying these effects. To end, macrophage line was stressed arsenite,...
Nasal application of soluble Ags leads to Ag-specific suppression systemic immune responses. This tolerance can be transferred naive mice by CD4(+) regulatory T cells (T(R) cells) from the spleen, but little is known about induction mucosal T(R) in vivo. To investigate nose-draining cervical lymph node (CLN), DO11.10 OVA TCR transgenic were BALB/c recipients. Within 48 h after nasal application, CLN, not peripheral node, had divided. Similarly, nonmucosal (i.m.) also induced proliferate...
Background The anti-inflammatory capacity of heat shock proteins (HSP) has been demonstrated in various animal models inflammatory diseases and patients. However, the mechanisms underlying this are poorly understood. Therefore, possible protective potential HSP70 its were studied proteoglycan (PG) induced arthritis (PGIA), a chronic relapsing, T cell mediated murine model arthritis. Methodology/Principal Findings immunization, 10 days prior to disease induction with PG, inhibited both...
The interleukin-7 (IL-7)-related cytokine thymic stromal lymphopoietin (TSLP) is a potent activator of myeloid dendritic cells, enhancing Th2-mediated hypersensitivity, and it has been implicated in the pathogenesis atopic diseases. Although intraarticular concentrations TSLP have shown to be increased patients with rheumatoid arthritis (RA), functional capacities are poorly studied. purpose this study was investigate effects administration receptor deficiency on immune activation, severity,...
Cellular therapies with tolerogenic antigen-presenting cells (tolAPC) show great promise for the treatment of autoimmune diseases and prevention destructive immune responses after transplantation. The methodologies generating tolAPC vary greatly between different laboratories, making it difficult to compare data from studies; thus constituting a major hurdle development standardised therapeutic products. Here we describe an initiative by members field generate minimum information model...
Innate-like murine B-1a cells are well known for their ability to secrete natural IgM. Their non-Ab mediated functions, including Ag presentation CD4(+) T cells, less explored. Using combined adoptive transfer experiments with peptide-pulsed peritoneal cavity (PerC)-derived and CFSE-labeled we show that present from the periphery in vivo. In vitro characterization, using co-cultures which or splenic B presented whole OVA protein OVA-specific Tg shows differentially promote intracellular...