A5003943728- Immune Cell Function and Interaction
- Heat shock proteins research
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Organ and Tissue Transplantation Research
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- COVID-19 Clinical Research Studies
- Genomics, phytochemicals, and oxidative stress
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Renal Transplantation Outcomes and Treatments
- Respiratory viral infections research
- Adenosine and Purinergic Signaling
- Cultural, Media, and Literary Studies
- Viral gastroenteritis research and epidemiology
- Tissue Engineering and Regenerative Medicine
- thermodynamics and calorimetric analyses
- Sepsis Diagnosis and Treatment
- Pharmacological Effects of Natural Compounds
- Facial Nerve Paralysis Treatment and Research
- Celiac Disease Research and Management
- Immunodeficiency and Autoimmune Disorders
- Diabetes and associated disorders
Harvard University
2015-2025
Massachusetts General Hospital
2020-2025
Brigham and Women's Hospital
2016-2023
Beth Israel Deaconess Medical Center
2014-2021
Fundação de Apoio à Universidade Federal de São Paulo
2020
Universidade Estadual Paulista (Unesp)
2020
Pontifícia Universidade Católica do Rio Grande do Sul
2009-2018
Renal Research Institute
2017
Universidade de Brasília
2016
Departamento de Ciência e Tecnologia
2013
Immune responses to heat shock proteins (Hsp) develop in virtually all inflammatory diseases; however, the significance of such is only now becoming clear. In experimental disease models, Hsp administration can prevent or arrest damage, and initial clinical trials patients with chronic diseases, peptides have been shown promote production anti-inflammatory cytokines, indicating immunoregulatory potential Hsp. Therefore, presence immune diseases be seen as an attempt system correct condition....
Xenotransplantation offers a potential solution to the organ shortage crisis. A 62-year-old hemodialysis-dependent man with long-standing diabetes, advanced vasculopathy, and marked dialysis-access challenges received gene-edited porcine kidney 69 genomic edits, including deletion of three glycan antigens, inactivation endogenous retroviruses, insertion seven human transgenes. The xenograft functioned immediately. patient's creatinine levels decreased promptly progressively, dialysis was no...
Immune-checkpoint inhibitors are emerging as revolutionary drugs for certain malignancies. However, blocking the co-inhibitory signals may lead to immune-related adverse events, mainly in spectrum of autoimmune diseases including colitis, endocrinopathies and nephritis. Here, we report a case 75-year-old man with metastatic malignant melanoma treated combination nivolumab (anti-PD1-antibody) ipilimumab (anti-CTLA-4 antibody) who developed systemic rash along severe acute tubulointerstitial...
Adoptive cell transfer of ex vivo expanded regulatory T cells (Tregs) has shown immense potential in animal models auto- and alloimmunity. However, the effective translation such Treg therapies to clinic been slow. Because homeostasis is known require continuous receptor (TCR) ligation exogenous interleukin-2 (IL-2), some investigators have explored use low-dose IL-2 injections increase endogenous responses. Systemic immunotherapy, however, can also lead activation cytotoxic lymphocytes...
Long-term organ transplant survival remains suboptimal, and life-long immunosuppression predisposes recipients to an increased risk of infection, malignancy, kidney toxicity. Promoting the regulatory arm immune system by expanding Tregs may allow minimization improve long-term graft outcomes. While low-dose IL-2 treatment can expand Tregs, it has a short half-life off-target expansion NK effector T cells, limiting its clinical applicability. Here, we designed humanized mutein with high Treg...
Face vascularized composite allografts (FVCAs) have helped patients with severe facial disfigurement, acute rejection now largely controlled through iatrogenic immunosuppression. However, little is known regarding the incidence and mechanism(s) of more long-term pathologic alterations in FVCAs that may affect function graft durability. Protocol surveillance biopsy specimens for up to an 8-year interval 7 who received at our institution revealed histopathologic evidence chronic rejection....
COVID-19 manifests as a milder disease in children than adults, but the underlying mechanisms are not fully characterized. Here we assess difference cellular or humoral immune responses of pediatric and adult patients to see if these factors contribute severity dichotomy. Children's non-specific profile is dominated by naive lymphocytes HLA-DR
Introduction Studies have shown reduced antiviral responses in kidney transplant recipients (KTRs) following SARS-CoV-2 mRNA vaccination, but data on post-vaccination alloimmune and against the Delta (B.1.617.2) variant are limited. Materials methods To address this issue, we conducted a prospective, multi-center study of 58 adult KTRs receiving mRNA-BNT162b2 or mRNA-1273 vaccines. We used multiple complementary non-invasive biomarkers for rejection monitoring including serum creatinine,...
A high-salt diet (HSD) in humans is linked to a number of complications, including hypertension and cardiovascular events. Whether HSD affects the immune response transplantation unknown. Using murine model, we investigated effect NaCl on alloimmune vitro vivo. Incremental concentrations augmented T cell proliferation settings both polyclonal allospecific stimulation. Feeding C57BL/6 wild-type recipients bm12 allografts led accelerated cardiac allograft rejection, despite similar mean BP...
Purpose: Extracellular Hsp70 has anti-inflammatory potential, demonstrated in different models of inflammatory diseases. We investigated probable mechanisms used by to down-regulate pro-inflammatory cytokines.
Scavenger receptor associated with endothelial cells I (SREC-I) was shown to be expressed in immune and play a role the endocytosis of peptides antigen presentation. As our previous studies indicated that SREC-I required intact Toll-like 4 (TLR4) expression for its functions tumor immunity, we examined potential interactions between these two receptors. We have here became TLR4 on binding bacterial lipopolysaccharides (LPS) RAW 264.7 HEK 293 overexpressing The receptors then internalized...
Tregs hold great promise as a cellular therapy for multiple immunologically mediated diseases, given their ability to control immune responses. The success of such strategies depends on the expansion healthy, suppressive ex vivo and in following transfer. In clinical studies, levels transferred decline sharply blood within few days have high rate apoptosis. Here, we describe new mechanism Treg self-inflicted damage. We show that granzymes A -B (GrA GrB), which are highly upregulated human...
Background Heat shock proteins (Hsps) are stress induced with immunomodulatory properties. The Hsp70 of Mycobacterium tuberculosis (TBHsp70) has been shown to have an anti-inflammatory role on rodent autoimmune arthritis models, and the protective effects were demonstrated be dependent interleukin-10 (IL-10). We previously observed that TBHsp70 inhibited maturation dendritic cells (DCs) IL-10 production by these cells, as well in synovial fluid cells. Methodology/Principal Findings...
Transplantation is the treatment of choice for many patients with end-stage organ disease. Despite advances in immunosuppression, long-term outcomes remain suboptimal, hampered by drug toxicity and immune-mediated injury, leading cause late graft loss. The development therapies that promote regulation while suppressing effector immunity imperative to improve survival minimize conventional immunosuppression. Notch signaling a highly conserved pathway pivotal T-cell differentiation function,...
Facial transplantation is a life‐changing procedure for patients with severe composite facial defects. However, skin the most immunogenic of all transplants, and better understanding immunological processes after paramount importance. Here, we describe six who underwent full at our institution, mean follow‐up 2.7 years. Seum, peripheral blood mononuclear cells, biopsy specimens were collected prospectively, detailed characterization their immune response (51 time points) was performed,...
Background: There is unmet need for non-invasive immunomonitoring to improve diagnosis and treatment of acute rejection in vascularized composite allotransplantation (VCA). Circulating matrix metalloproteinase 3 (MMP3) was described as a candidate biomarker predict response clinical VCA. However, larger validation studies are yet be reported allow more definitive conclusions. Methods: We retrospectively measured MMP3 levels using ELISA total 140 longitudinal serum samples from 6 internal...