A5062951393- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Pediatric health and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Inhalation and Respiratory Drug Delivery
- Allergic Rhinitis and Sensitization
- Inflammatory mediators and NSAID effects
- Inflammasome and immune disorders
- Neonatal Respiratory Health Research
- Pleural and Pulmonary Diseases
- Genomics, phytochemicals, and oxidative stress
- Eicosanoids and Hypertension Pharmacology
- Respiratory and Cough-Related Research
- Respiratory Support and Mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Fatty Acid Research and Health
- Advanced Chemical Sensor Technologies
- Antimicrobial Peptides and Activities
- Immune cells in cancer
- Electrochemical sensors and biosensors
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
National Research Council
2014-2024
Istituto di Farmacologia Traslazionale
2022-2024
National Academies of Sciences, Engineering, and Medicine
2006-2023
Institute for Biomedical Research and Innovation
2019-2022
Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy
2011-2022
University of Milan
1998-2020
Elisa (Finland)
2019
Istituto di Biometeorologia
2019
University of Palermo
2015
University of Palermo
1996-2004
We assessed whether transforming growth factor- β (TGF- ), a fibrogenic factor, may be involved in remodeling of asthma and chronic bronchitis; its expression was compared with that epidermal factor (EGF) granulocyte macrophage colony-stimulating (GM-CSF) bronchial mucosal biopsies from 13 normal subjects, 24 asthmatics, 19 patients bronchitis. TGF- immunoreactivity highly increased epithelium submucosa those bronchitis to lesser extent asthmatics. By comparison, EGF significantly the...
Airway epithelium is emerging as a regulator of innate immune responses to variety insults including cigarette smoke. The main goal this study was explore the effects smoke extracts (CSE) on Toll-like receptor (TLR) expression and activation in human bronchial epithelial cell line (16-HBE). CSE increased TLR4 lipopolysaccharide (LPS) binding, nuclear factor-kappaB (NF-kappaB) activation, release interleukin-8 (IL-8) chemotactic activity toward neutrophils. It did not induce TLR2 or...
Background Altered pulmonary defenses in chronic obstructive disease (COPD) may promote distal airways bacterial colonization. The expression/activation of Toll Like receptors (TLR) and beta 2 defensin (HBD2) release by epithelial cells crucially affect defence mechanisms. Methods expression TLR4 HBD2 was assessed surgical specimens from current smokers COPD (s-COPD; n = 17), ex-smokers (ex-s-COPD; 8), without (S; 12), non-smoker non-COPD subjects (C; 13). Results In airways, s-COPD highly...
The NLRP3 inflammasome is formed by the sensor NLRP3, adaptor ASC, and pro-caspase-1. Assembly activation of trigger caspase-1-dependent cleavage pro-IL-1β pro-IL-18 into their secreted forms. Cigarette smoke a risk factor for chronic inflammatory diseases associated with macrophage dysfunction. impact cigarette on NLRP3-dependent responses in macrophages largely unknown. Herein, we investigated effects extract (CSE) human monocyte-derived (MDMs) THP-1 cells stimulated lipopolysaccharide...
Immunoglobulin G (IgG), a type of antibody, represents approximately 75% serum antibodies in humans, and is the most common antibody found blood circulation. Consequently, development simple, fast reliable systems for IgG detection, which can be achieved using electrochemical sandwich-type immunosensors, considerable interest. In this study we have developed an immunosensor human (H)-IgG inexpensive very simple fabrication method based on ZnO nanorods (NRs) obtained through electrodeposition...
Abstract Inflammation and ageing are intertwined in chronic obstructive pulmonary disease ( COPD ). The histone deacetylase SIRT 1 the related activation of FoxO3 protect from regulate inflammation. role 1/FoxO3 is largely unknown. This study evaluated whether cigarette smoke, by modulating axis, affects airway epithelial pro‐inflammatory responses. Human bronchial cells (16 HBE ) primary PBEC s) patients controls were treated with/without smoke extract CSE ), Sirtinol or si RNA . 1, NF ‐κB...
Hydrogen peroxide (H
The pleural space is a potential compartment between the lung and chest wall that becomes filled with fluid inflammatory cells in number of respiratory diseases. In an attempt to understand one aspect process space, we compared responses three different diseases (congestive heart failure [CHF], tuberculosis [TB], cancer). Large concentrations interleukin-8 (IL-8) were detected cancer TB effusions, but not CHF. Surprisingly, concentration IL-8 correlated best lymphocyte recruitment neutrophil...
Cigarette smoke extracts (CSE) induce oxidative stress, an important feature in chronic obstructive pulmonary disease (COPD), and stress contributes to the poor clinical efficacy of corticosteroids COPD patients. Carbocysteine, antioxidant mucolytic agent, is effective reducing severity rate exacerbations The effects carbocysteine on CSE-induced bronchial epithelial cells as well comparison these with those fluticasone propionate are unknown. present study was aimed assess (10(-4) M) cell...
We evaluated the levels of 15(S)-hydroxyeicosatetraenoic acid [15(S)-HETE] and expression 15-lipoxygenase (15-LO) mRNA in induced sputum obtained from 10 control 15 chronic bronchitis subjects. 15(S)-HETE was by reverse phase high-performance liquid chromatography separation followed specific RIA. 15-LO determined primed situ labeling. The both soluble cell-associated resulted significantly higher than percentage cells expressing subjects (P < 0.01). Double staining for cell type markers...
Rationale. Severe asthma is characterized by inadequate symptom control and high rate of inflammation despite doses steroids. Omalizumab, a recombinant humanized monoclonal anti-IgE, provides new therapeutic strategy in severe allergic asthma. Aims. This study was aimed to assess whether long-term treatment with omalizumab improved clinical asthmatics. Methods. We investigated effects on outcomes evaluating seven asthmatic patients who were treated for 7 years add-on omalizumab. Number...
Aim: Therapeutic efficacy of pulmonary diseases is often limited and drug delivery systems offer new solutions to clinical problems. Solid lipid microparticles (SLMs) are suggested as for the therapeutics lung as, because their size, they able deposit into secondary bronchi. Materials & methods: Here, we describe two novel different SLMs using chitosan alginate such mucoadhesive polymers also studied biocompatibility effectiveness compared with free in controlling senescence inflammatory...
Cigarette smoke exposure, increasing Toll-like receptor 4 (TLR4) and reactive oxygen species (ROS), promotes inflammatory responses in airway epithelial cells. Chronic inflammation, microRNA (miRNA), oxidative stress are associated with cancer development.The present study was aimed to explore whether cigarette altering miR-21 expression, promoted tumorigenesis processes cells.Airway normal cells (16HBE A549) were exposed extracts (CSE) or with/without agomiR-21, then it assessed: a)...