A5025358926- IL-33, ST2, and ILC Pathways
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Immune Cell Function and Interaction
- Gastrointestinal motility and disorders
- Inflammasome and immune disorders
- T-cell and B-cell Immunology
- Advanced Chemical Sensor Technologies
- Biomarkers in Disease Mechanisms
- Analytical Chemistry and Sensors
- Heme Oxygenase-1 and Carbon Monoxide
- Gas Sensing Nanomaterials and Sensors
- Pancreatic and Hepatic Oncology Research
- Electrochemical sensors and biosensors
- Fuel Cells and Related Materials
- Animal Genetics and Reproduction
- Immune Response and Inflammation
- Advanced biosensing and bioanalysis techniques
- Psoriasis: Treatment and Pathogenesis
- CRISPR and Genetic Engineering
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Adipokines, Inflammation, and Metabolic Diseases
- Gastrointestinal disorders and treatments
- Transgenic Plants and Applications
- Genetic factors in colorectal cancer
Ri.MED
2022-2025
European Institute of Oncology
2020-2023
University of Milan
2018-2019
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2019
University of Milano-Bicocca
2016-2018
Abstract Background The gut microbiota plays a central role in host physiology and several pathological mechanisms humans. Antibiotics compromise the composition functions of inducing long-lasting detrimental effects on host. Recent studies suggest that efficacy different clinical therapies depends action microbiota. Here, we investigated how antibiotic treatments affect ability to control intestinal inflammation upon fecal transplantation an experimental colitis model ex vivo experiments...
Different gastrointestinal disorders, including inflammatory bowel diseases (IBD), have been linked to alterations of the gut microbiota composition, namely dysbiosis. Fecal transplantation (FMT) is considered an encouraging therapeutic approach for ulcerative colitis patients, mostly as a consequence normobiosis restoration. We recently showed that effects FMT during acute experimental are functional modulation mucosal immune system and composition. Here we analysed administration chronic...
// Gabriele Romano 1 , Ludovica Santi Maria Rosaria Bianco 2 Rita Giuffrè Mariateresa Pettinato Cristina Bugarin 3 Garanzini Leonilde Savarese 4 Silvia Leoni Grazia Cerrito Biagio Eugenio Leone Giuseppe Gaipa Emanuela Grassilli Michele Papa Marialuisa Lavitrano Roberto Giovannoni Department of Surgery and Translational Medicine, University Milano-Bicocca, 20900, Monza, Italy c/o Mental Physical Health Preventive Second Naples, 80138, M. Tettamanti Research Center, Pediatric Clinic, Milano...
Chronic obstructive pulmonary disease (COPD) is a progressive lung characterized by obstructed airflow, airway remodeling, and inflammation, with cigarette smoke (CS) exposure being the main risk factor. While CS extract (CSE) has been shown to activate caspases in various cell types, role of human fibroblasts (hLFs), COPD remains poorly understood. Recent studies have linked extracellular matrix (ECM) remodeling skin kidney fibrosis. Caspase activation can be triggered oxidative stress,...
Hydrogen peroxide (H
Inflammatory bowel disease (IBD) pathogenesis has been linked to the aberrant activation of Gut-associated lymphoid tissues against components intestinal microbiota. Although contribution CD4 + T helper cells inflammatory processes is being increasingly acknowledged, functional engagement human invariant natural killer (iNKT) still poorly defined. Here, we evaluated characteristics iNKT during IBD and exploit role mucosa-associated microbiota recognition in triggering cells’ pro-inflammatory...
iNKT cells account for a relevant fraction of effector T-cells in the intestine and are considered an attractive platform cancer immunotherapy. Although cytotoxic lymphocytes, their functional role colorectal (CRC) is still controversial, limiting therapeutic use. Thus, we examined immune cell composition phenotype CRC lesions patients (n = 118) different murine models. High-dimensional single-cell flow-cytometry, metagenomics, RNA sequencing experiments revealed that enriched tumor lesions....
Invariant natural killer T [iNKT] cells perform pleiotropic functions in different tissues by secreting a vast array of pro-inflammatory and cytotoxic molecules. However, the presence function human intestinal iNKT capable immunomodulatory molecules such as IL-10 has never been reported so far. Here we describe for first time IL10-producing [NKT10 cells] lamina propria healthy individuals Crohn's disease [CD] patients.Frequency phenotype NKT10 were analysed ex vivo from specimens [n = 17]...
Abstract Cigarette smoking impairs the lung innate immune response making smokers more susceptible to infections and severe symptoms. Dysregulation of cell death is emerging as a key player in chronic inflammatory conditions. We have recently reported that short exposure human monocyte-derived macrophages (hMDMs) cigarette smoke extract (CSE) altered TLR4-dependent lipopolysaccharide (LPS). CSE caused inhibition MyD88-dependent activation TRIF/caspase-8/caspase-1 pathway leading Gasdermin D...
T helper 17 [Th17] cells are crucially involved in the immunopathogenesis of inflammatory bowel diseases humans. Nevertheless, pharmacological blockade interleukin 17A [IL17A], Th17 signature cytokine, yielded negative results patients with Crohn’s disease [CD], and attempts to elucidate determinants cells’ pathogenicity gut have so far proved unsuccessful. Here, we aimed identify functionally validate pathogenic intestinal IL-17–producing cells. In vivo–generated murine were adoptively...
High concentrations of H2O2, indicative increased oxidative stress in the lung, are observed exhaled breath individuals affected by different respiratory diseases. Therefore, measuring H2O2 exhale represents a promising and non-invasive approach for monitoring onset progression these Herein, we have developed an innovative, inexpensive, easy-to-use device measurement breath. The is based on silver layer covered with electrodeposited thin film chitosan, that ensures wettability sensor humid...
Cigarette smoke (CS) is a major risk factor for chronic lung diseases and promotes activation of pattern recognition receptors in the bronchial epithelium. NOD-like receptor family, pyrin domain-containing 3 (NLRP3) whose leads to caspase-1 cleavage, maturation/release IL-1β IL-18, eventually pyroptosis. Whether NLRP3 inflammasome participates CS-induced inflammation epithelial cells still unclear. Herein, we evaluated involvement inflammatory responses human primary cells. To this purpose,...
Quantification of oxidative stress is a challenging task that can help in monitoring chronic inflammatory respiratory airway diseases. Different studies be found the literature regarding development electrochemical sensors for H2O2 cell culture medium to quantify stress. However, there are very limited data impact on quantification H2O2. In this work, we studied effect different media (RPMI, MEM, DMEM, Ham’s F12 and BEGM/DMEM) The used electrode based reduced graphene oxide (rGO) gold...
Abstract iNKT cells account for a relevant fraction of effector T-cells in the intestine. Although are cytotoxic lymphocytes, their role colorectal cancer (CRC) remains controversial. From analysis colonic LPMCs human and murine CRC specimens we report that tumor-infiltrating characterized by an IL17/GM-CSF pro-tumorigenic phenotype, while maintaining properties adjacent non-tumoral tissue. Exposure to tumor-associated pathobiont Fusobacterium nucleatum blunted capability enhanced...