A5002730356- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Blood disorders and treatments
- Immunodeficiency and Autoimmune Disorders
- Chronic Myeloid Leukemia Treatments
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Blood groups and transfusion
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Protein Tyrosine Phosphatases
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Viral-associated cancers and disorders
- Organ Transplantation Techniques and Outcomes
- Childhood Cancer Survivors' Quality of Life
- Cytomegalovirus and herpesvirus research
- Erythrocyte Function and Pathophysiology
- Histiocytic Disorders and Treatments
- Galectins and Cancer Biology
- Tumors and Oncological Cases
- Single-cell and spatial transcriptomics
Istituti di Ricovero e Cura a Carattere Scientifico
2023-2024
Azienda Ospedaliera San Gerardo
2023-2024
University of Milano-Bicocca
2012-2022
Centre Hospitalier Universitaire de Saint-Étienne
2020
Extracorporeal photochemotherapy (ECP) produces clinical improvements in refractory/resistant graft-versus-host disease (GvHD). Immunological mechanisms of ECP are still under investigation.We have evaluated the changes frequency and immunophenotype circulating regulatory T cells (T-regs) 10 patients undergoing allogeneic hematopoietic stem cell transplantation, receiving for acute (n=4) or chronic (n=6) GvHD. T-regs were monitored expression surface CD4, CD25, GITR, CD45RO, CD62L...
Background. Immune mechanisms of extracorporeal photochemotherapy (ECP) in refractory/resistant graft-versus-host disease (GvHD) are complex. We have previously analyzed the role CD4+CD25+Foxp3+ regulatory T cells (T-regs). Methods. In current study, we enlarged size population (n=27; chronic GvHD=18, acute GvHD=9) for a median follow-up 24 months. T-regs were monitored CD4, CD25, glucocorticoid-induced tumor necrosis factor receptor (GITR), CD62L, CCR7, Foxp3, and STAT-5. analysis by...
// Gabriele Romano 1 , Ludovica Santi Maria Rosaria Bianco 2 Rita Giuffrè Mariateresa Pettinato Cristina Bugarin 3 Garanzini Leonilde Savarese 4 Silvia Leoni Grazia Cerrito Biagio Eugenio Leone Giuseppe Gaipa Emanuela Grassilli Michele Papa Marialuisa Lavitrano Roberto Giovannoni Department of Surgery and Translational Medicine, University Milano-Bicocca, 20900, Monza, Italy c/o Mental Physical Health Preventive Second Naples, 80138, M. Tettamanti Research Center, Pediatric Clinic, Milano...
To diagnose juvenile myelomonocytic leukemia (JMML) is sometimes challenging, because around 10% of patients lack molecular abnormalities affecting Ras-MAPK (mitogen-activated protein kinase) pathway and other diseases such as cytomegalovirus infection can mimic clinical signs JMML. In order to validate a phospho-specific flow cytometry assay assessing phospho-signal transducer activator transcription factor 5 (p-STAT5) new diagnostic tool for JMML, we examined 22 samples from children with...
Pediatric T-ALL patients have a worse outcome compared to BCP-ALL and they could benefit from new prognostic marker identification. Alteration of CRLF2 gene, hallmark correlated with poor in BCP-ALL, has not been reported T-ALL.We analyzed expression 212 pediatric enrolled AIEOP-BFM ALL2000 study Italian German centers.Seventeen out 120 (14.2%) presented mRNA 5 times higher than the median (CRLF2-high); had significantly inferior event-free survival (41.2%±11.9 vs. 68.9%±4.6, p=0.006)...
Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide toward the relevant panel and final diagnosis. In this study, we validated algorithm automated (database-supported) gating identification (AGI tool) cell subsets within samples stained with ALOT. A reference database normal peripheral blood (PB, n = 41) bone marrow (BM; 45) analyzed ALOT was constructed, served as a AGI tool automatically...
Triple Negative Breast Cancer (TNBC) is an aggressive neoplasia with median Overall Survival (OS) less than two years. Despite the availability of new drugs, chance survival these patients did not increase. The combination low doses drugs in a metronomic schedule showed efficacy clinical trials, exhibiting anti-proliferative and anti-tumour activity. In Victor-2 study we recently evaluated (mCHT) Capecitabine (CAPE) Vinorelbine (VNR) breast cancer showing disease control rate...
Shwachman–Diamond syndrome (SDS) is a rare inherited disorder characterized by bone marrow (BM) dysfunction and exocrine pancreatic insufficiency. SDS patients have an increased risk for myelodisplastic acute myeloid leukemia. Mesenchymal stem cells (MSCs) are the key component of hematopoietic microenvironment relevant in inducing genetic mutations leading to However, their role still unexplored. We demonstrated that morphology, growth kinetics expression surface markers MSCs from...
// Valeria Cazzaniga 1 , Cristina Bugarin Michela Bardini Marco Giordan 2 Geertruy te Kronnie Giuseppe Basso Andrea Biondi Grazia Fazio 1, * Giovanni Centro Ricerca Tettamanti, Clinica Pediatrica, Università di Milano-Bicocca, Ospedale San Gerardo/Fondazione MBBM, Monza 20900, Italy Laboratory of Oncohematology, Department Women’s and Children’s Health, University Padova, Padova 35128, These authors contributed equally to this work Correspondence to: Cazzaniga, e-mail:...
Ischemia-reperfusion injury (IRI) and oxidative stress still limit the survival of cells organs in xenotransplantation models. Ectonucleotidases play an important role inflammation IRI transplantation settings. We tested potential protective effects derived by co-expression two main vascular ectonucleotidases, ecto-5'-nucleotidase (E5NT) ecto nucleoside triphosphate diphosphohydrolase 1 (ENTPD1), vitro model H2O2-induced cytotoxicity. produced a dicistronic plasmid (named pCX-DI-2A) for...
Abstract Background The PI3K/Akt/mTOR (PI3K) signaling pathway has a crucial role in T‐cell acute lymphoblastic leukemias (T‐ALLs). Although loss‐of‐function of phosphatase and tensin homolog (PTEN) is common event pediatric T‐ALLs, the exact this tumor suppressor T‐ALL development yet to be defined. Methods Here, we report an optimized cytometric method for accurate proteomic profiling leukemic blasts at single‐cell level. We determined expression PI3K JAK–STAT components both primary...
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive cancer arising from lymphoblasts of origin. While TALL accounts for only 15% childhood and 25% adult ALL, 30% patients relapse with a poor outcome. Targeted therapy resistant high-risk pediatric T-ALL therefore urgently needed, together precision medicine tools allowing the testing efficacy in patient samples. Furthermore, leukemic cell heterogeneity requires drug response assessment at single-cell level. Here we used mass...
Shwachman-Diamond syndrome (SDS) is a rare multi-organ recessive disease mainly characterised by pancreatic insufficiency, skeletal defects, short stature and bone marrow failure (BMF). As in many other BMF syndromes, SDS patients are predisposed to develop number of haematopoietic malignancies, particularly myelodysplastic acute myeloid leukaemia. However, the mechanism cancer predisposition only partially understood. In light emerging role mesenchymal stromal cells (MSCs) regulation...
Several biomedical applications, such as xenotransplantation, require multiple genes simultaneously expressed in eukaryotic cells. Advances genetic engineering technologies have led to the development of efficient polycistronic vectors based on use 2A self-processing oligopeptide. The aim this work was evaluate protective effects simultaneous expression a novel combination anti-inflammatory human genes, ENTPD1, E5NT and HO-1, We produced an F2A system-based multicistronic construct express...
Children with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) overexpressing the CRLF2 gene (hiCRLF2) have poor prognosis. protein overexpression leads to activated JAK/STAT signaling and trials are underway using JAK inhibitors overcome treatment failure. Pre-clinical studies indicated limited efficacy of single inhibitors, thus additional pathways must be targeted in hiCRLF2 cells. To identify networks, we used single-cell mass cytometry examine 15 BCP-ALL primary patient samples....