A5064348968- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Molecular Biology Techniques and Applications
- Sphingolipid Metabolism and Signaling
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- CAR-T cell therapy research
- Viral-associated cancers and disorders
- Ovarian cancer diagnosis and treatment
- Acute Lymphoblastic Leukemia research
- Renal and related cancers
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Cell Image Analysis Techniques
- Advanced Biosensing Techniques and Applications
- Hematological disorders and diagnostics
University of Milano-Bicocca
2015-2024
Azienda Ospedaliera San Gerardo
1987-2023
Columbia University
2000-2023
Cancer Genetics (United States)
2001-2023
Herbert Irving Comprehensive Cancer Center
2023
Agostino Gemelli University Polyclinic
2023
Azienda Socio Sanitaria Territoriale Lariana
2016-2018
KU Leuven
2016-2017
European Institute of Oncology
1997-2017
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2000-2015
Malignant cells often display defects in autophagy, an evolutionarily conserved pathway for degrading long-lived proteins and cytoplasmic organelles. However, as yet, there is no genetic evidence a role of autophagy genes tumor suppression. The beclin 1 gene monoallelically deleted 40–75% cases human sporadic breast, ovarian, prostate cancer. Therefore, we used targeted mutant mouse model to test the hypothesis that monoallelic deletion promotes tumorigenesis. Here show heterozygous...
Abstract The monoclonal antibody Ki‐67 reacts with a human nuclear cell proliferation‐associated antigen that is expressed in all active parts of the cycle. Recently we have raised antibodies, MIB 1–3, against recombinant antigen. These antibodies are true equivalents, as demonstrated by immunostaining fresh specimens, biochemistry, and molecular biological techniques. Formalin‐fixed, paraffin‐embedded sections routinely processed for immunohistochemistry failed to stain 2. Antibodies 1 3...
B cell-derived chronic lymphocytic leukemia (B-CLL) represents a common malignancy whose cell derivation and pathogenesis are unknown. Recent studies have shown that >50% of CLLs display hypermutated immunoglobulin variable region (IgV) sequences more favorable prognosis, suggesting they may represent distinct subset which transited through germinal centers (GCs), the physiologic site IgV hypermutation. To further investigate phenotype CLLs, their cellular relationship to normal cells, we...
Abstract Enzymatic and non‐enzymatic treatments for antigen unmasking on formalin‐fixed, paraffin‐embedded, dewaxed sections were optimized compared by the use of a panel antibodies diagnostic relevance (anti‐cytokeratins, vimentin. S‐100. T‐ B‐cell receptors, Ki‐67/MIB 1, muscle actin). Non‐enzymatic was obtained boiling slides in microwave oven 0.01 M salt solution (pH 6) or 6 urea. Trypsin pronase digestion used comparison found to be necessary some reagents. The investigation then...
Abstract Immunohistochemical evaluation of 200 primary breast cancers with the anti‐p53 mouse monoclonal antibody (MAb) PAb421 showed positivity in nuclei malignant cells 31 cases (15.5%). + were significantly more frequently epidermal growth factor receptor (EGF‐R)‐positive (67.7%; p < 0.001) and estrogen (ER)‐negative (73.3%; 0.001); they displayed surface histocompatibility class‐1 (80.6%; 0.01) II (74.2%; 0.05) antigens. Low values for progesterone (mean 67.20 ± 25.2 fmol/mg; a high...
Germ line mutations in DNA mismatch repair genes including MLH1 cause hereditary nonpolyposis colon cancer. To understand the role of normal growth and development, we generated mice that have a null mutation this gene. Mice homozygous for show replication error phenotype, extracts these cells are deficient activity. Homozygous mutant males mating behavior but no detectable mature sperm. Examination meiosis reveals enter meiotic prophase arrest at pachytene. females estrous cycles...
The germinal center (GC) reaction is crucial for T cell-dependent immune responses and targeted by B cell lymphomagenesis. Here we analyzed the transcriptional changes that occur in cells during GC transit (naïve → centroblasts centrocytes memory cells) gene expression profiling. Naïve cells, characterized of cycle-inhibitory antiapoptotic genes, become inducing an atypical proliferation program lacking c-Myc expression, switching to a proapoptotic program, down-regulating cytokine,...
Acute promyelocytic leukemia (APL) is associated with reciprocal chromosomal translocations involving the retinoic acid receptor α (RARα) locus on chromosome 17. In majority of cases, RARα translocates and fuses (PML) gene located 15. The resulting fusion genes encode two structurally unique PML/RARα RARα/PML proteins as well aberrant PML products, respective pathogenetic roles which have not been elucidated. We generated transgenic mice in protein specifically expressed...
B lymphocyte-induced maturation protein-1 (Blimp-1) is a transcriptional repressor that sufficient to trigger terminal differentiation in the cell lymphoma BCL-1. In this study, we have determined expression pattern of Blimp-1 vivo primary and secondary lymphoid organs humans immunized mice. expressed plasma cells derived from either T-independent or T-dependent response undergone isotype switching those resulting immunization. also present long-lived residing bone marrow. However, was not...
A new human leukaemic cell line (M-O7) with the phenotypic characteristics of CFU-mega is described. Its cells are positive for T200 leucocyte common antigen (LCA) and negative MAbs recognizing T B mature myelomonocytic antigens. In contrast, they react antigenic determinants to multi-lineage (CD13, CD33, CD34) bipotent erythromegakaryoblastic (CD36, H25) haemopoietic precursors, specific platelet glycoproteins (CD41w, CD42w). small proportion (10%) were large multinucleated, on...
Hodgkin lymphoma (HL) is a malignancy of unknown pathogenesis. The malignant and Reed/Sternberg (HRS) cells derive from germinal center B (or rarely, T cells) but have heterogeneous largely uncharacterized phenotype. Using microarrays, we compared the gene expression profile four HL cell lines with profiles main subsets non-HLs to find out whether HRS cells, despite their described heterogeneity, show distinct expression, study relationship other normal identify genes aberrantly or...
Germinal centers (GCs) represent the main sites for generation of high-affinity, class-switched antibodies during T cell-dependent antibody responses. To study gene function specifically in GC B cells, we generated Cγ1-cre mice which expression Cre recombinase is induced by transcription Ig γ1 constant region segment (Cγ1). In these mice, Cre-mediated recombination at fas , β, IgH and Rosa26 loci occurred cells as early 4 days after immunization with antigens involved >85% peak reaction....
Hematopoietic stem cells in the bone marrow give rise to lymphoid progenitors, which subsequently differentiate into B and T lymphocytes. Here we show that proto-oncogene LRF plays an essential role versus cell-fate decision. We demonstrate is key for instructing early progenitors mice develop lineage by repressing cell-instructive signals produced signal protein, Notch. propose a new model commitment, acts as master regulator of cell's determination lineage.
Hodgkin lymphoma (HL) is a malignancy of unknown pathogenesis. The malignant and Reed/Sternberg (HRS) cells derive from germinal center B (or rarely, T cells) but have heterogeneous largely uncharacterized phenotype. Using microarrays, we compared the gene expression profile four HL cell lines with profiles main subsets non-HLs to find out whether HRS cells, despite their described heterogeneity, show distinct expression, study relationship other normal identify genes aberrantly or...