Angelika Eggert
A5059343286- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer therapeutics and mechanisms
- Protein Degradation and Inhibitors
- Childhood Cancer Survivors' Quality of Life
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Lung Cancer Research Studies
- Signaling Pathways in Disease
- Advanced Breast Cancer Therapies
- Ubiquitin and proteasome pathways
- Genetics, Bioinformatics, and Biomedical Research
- CAR-T cell therapy research
- Neuroendocrine Tumor Research Advances
- Cancer Genomics and Diagnostics
- Glioma Diagnosis and Treatment
- Virus-based gene therapy research
- Ocular Oncology and Treatments
- Testicular diseases and treatments
- Sarcoma Diagnosis and Treatment
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Chromatin Remodeling and Cancer
- DNA Repair Mechanisms
- Histone Deacetylase Inhibitors Research
Charité - Universitätsmedizin Berlin
2016-2025
German Cancer Society
2015-2025
Deutschen Konsortium für Translationale Krebsforschung
2016-2025
German Cancer Research Center
2015-2025
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2016-2025
Heidelberg University
2016-2025
Humboldt-Universität zu Berlin
2017-2025
Freie Universität Berlin
2017-2025
Essen University Hospital
2007-2023
Orthopädische Universitätsklinik
2022-2023
Pan-cancer analyses that examine commonalities and differences among various cancer types have emerged as a powerful way to obtain novel insights into biology. Here we present comprehensive analysis of genetic alterations in pan-cancer cohort including 961 tumours from children, adolescents, young adults, comprising 24 distinct molecular cancer. Using standardized workflow, identified marked terms mutation frequency significantly mutated genes comparison previously analysed adult cancers....
Aberrant epigenetic changes in DNA methylation and histone acetylation are hallmarks of most cancers, whereas was previously considered to be irreversible less versatile. Recently, several demethylases were identified catalyzing the removal methyl groups from H3 lysine residues thereby influencing gene expression. Neuroblastomas continue remain a clinical challenge despite advances multimodal therapy. Here, we address functional significance chromatin-modifying enzyme lysine-specific...
Purpose For a comprehensive overview of the genetic alterations neuroblastoma, their association and clinical significance, we conducted whole-genome DNA copy number analysis. Patients Methods A series 493 neuroblastoma (NB) samples was investigated by array-based comparative genomic hybridization in two consecutive steps (224, then 269 patients). Results Genomic analysis identified several types profiles. Tumors presenting exclusively whole-chromosome variations were associated with...
A systematic look at a childhood tumor Neuroblastomas—the most common type in infants—develop from fetal nerve cells, and their clinical course is highly variable. Some neuroblastomas are fatal despite treatment, whereas others respond well to treatment some undergo spontaneous regression without treatment. Ackermann et al. sequenced more than 400 pretreatment identified molecular features that characterize the three distinct outcomes. Low-risk tumors lack telomere maintenance mechanisms,...
Abstract INFORM is a prospective, multinational registry gathering clinical and molecular data of relapsed, progressive, or high-risk pediatric patients with cancer. This report describes long-term follow-up 519 in whom alterations were evaluated according to predefined seven-scale target prioritization algorithm. Mean turnaround time from sample receipt was 25.4 days. The highest priority level observed 42 (8.1%). Of these, 20 received matched targeted treatment median progression-free...
Abstract MYCN amplification drives one in six cases of neuroblastoma. The supernumerary gene copies are commonly found on highly rearranged, extrachromosomal circular DNA (ecDNA). exact amplicon structure has not been described thus far and the functional relevance its rearrangements is unknown. Here, we analyze using short-read Nanopore sequencing chromatin landscape ChIP-seq, ATAC-seq Hi-C. This reveals two distinct classes amplicons which explain regulatory requirements for...
Abstract Extrachromosomal DNAs (ecDNAs) are common in cancer, but many questions about their origin, structural dynamics and impact on intratumor heterogeneity still unresolved. Here we describe single-cell extrachromosomal circular DNA transcriptome sequencing (scEC&T-seq), a method for parallel of full-length mRNA from single cells. By applying scEC&T-seq to cancer cells, intercellular differences ecDNA content while investigating transcriptional impact. Oncogene-containing ecDNAs...
MYCN amplification is a common feature of aggressive tumour biology in neuroblastoma. The transcription factor has been demonstrated to induce or repress expression numerous genes. MicroRNAs (miRNA) are recently discovered class short RNAs that translation and promote mRNA degradation by sequence-specific interaction with mRNA. Here, we sought analyse the role regulation miRNA expression. Using microarray containing 384 different miRNAs set 160 real-time PCR assays validate results, 7 were...
Small non-coding RNAs, in particular microRNAs(miRNAs), regulate fine-tuning of gene expression and can act as oncogenes or tumor suppressor genes. Differential miRNA has been reported to be functional relevance for biology. Using next-generation sequencing, the unbiased absolute quantification small RNA transcriptome is now feasible. Neuroblastoma(NB) an embryonal with highly variable clinical course. We analyzed transcriptomes five favorable unfavorable NBs using SOLiD generating a total...
ALK inhibitors induce complete tumor regression in a mouse model of ALK-driven neuroblastoma.
Targeting BET proteins was previously shown to have specific antitumoral efficacy against MYCN-amplified neuroblastoma. We here assess the therapeutic of inhibitor, OTX015, in preclinical neuroblastoma models and extend knowledge on role BRD4 MYCN-driven neuroblastoma.The OTX015 assessed vitro vivo human murine To study effects inhibition context high MYCN levels, ectopically expressed cells. The effect BRD4-regulated transcriptional pause release analyzed using H3K27Ac chromatin...