Caroline Hutter
A5009057245- Histiocytic Disorders and Treatments
- Childhood Cancer Survivors' Quality of Life
- Radiation Therapy and Dosimetry
- Neuroblastoma Research and Treatments
- Testicular diseases and treatments
- Extracellular vesicles in disease
- RNA regulation and disease
- Glioma Diagnosis and Treatment
- Mast cells and histamine
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Viral-associated cancers and disorders
- Genetic and rare skin diseases.
- Cancer Genomics and Diagnostics
- Vascular Malformations and Hemangiomas
- Parvovirus B19 Infection Studies
- Phagocytosis and Immune Regulation
- Genital Health and Disease
- Polyomavirus and related diseases
- Cellular Mechanics and Interactions
- Vascular Tumors and Angiosarcomas
- DNA Repair Mechanisms
- Sarcoma Diagnosis and Treatment
- PARP inhibition in cancer therapy
- Skin and Cellular Biology Research
St Anna Children's Hospital
2016-2025
Medical University of Vienna
2016-2025
St. Anna Children's Cancer Research Institute
2012-2024
University of Vienna
1996-2023
Medical University of Graz
2022
Boston Children's Hospital
2010
Research Institute of Molecular Pathology
2002-2008
Vienna Biocenter
2004
Martin Luther University Halle-Wittenberg
2003
Cancer Research UK
2002
Abstract INFORM is a prospective, multinational registry gathering clinical and molecular data of relapsed, progressive, or high-risk pediatric patients with cancer. This report describes long-term follow-up 519 in whom alterations were evaluated according to predefined seven-scale target prioritization algorithm. Mean turnaround time from sample receipt was 25.4 days. The highest priority level observed 42 (8.1%). Of these, 20 received matched targeted treatment median progression-free...
PURPOSE Off-label use of vemurafenib (VMF) to treat BRAF V600E mutation–positive, refractory, childhood Langerhans cell histiocytosis (LCH) was evaluated. PATIENTS AND METHODS Fifty-four patients from 12 countries took VMF 20 mg/kg/d. They were classified according risk organ involvement: liver, spleen, and/or blood cytopenia. The main evaluation criteria adverse events (Common Terminology Criteria for Adverse Events [version 4.3]) and therapeutic responses Disease Activity Score. RESULTS...
Sequencing of cell-free DNA in the blood cancer patients (liquid biopsy) provides attractive opportunities for early diagnosis, assessment treatment response, and minimally invasive disease monitoring. To unlock liquid biopsy analysis pediatric tumors with few genetic aberrations, we introduce an integrated genetic/epigenetic method demonstrate its utility on 241 deep whole-genome sequencing profiles 95 Ewing sarcoma 31 other sarcomas. Our achieves sensitive detection classification...
Integrins are ubiquitous transmembrane receptors that play crucial roles in cell-cell and cell-matrix interactions. In this study, we have determined the effects of loss β1 integrins keratinocytes vitro during cutaneous wound repair. Flow cytometry cultured β1-deficient confirmed absence showed downregulation α6β4 but not αv integrins. β1-null were characterised by poor adhesion to various substrates, a reduced proliferation rate strongly impaired migratory capacity. vivo, caused severe...
Liquid biopsies as a minimally invasive approach have the potential to revolutionize molecular diagnostics. Yet, although protocols for sample handling and isolation of circulating tumor DNA (ctDNA) are numerous, comprehensive guidelines diagnostics research considering all aspects real-life multicenter clinical studies currently not available. These include limitations in volume, transport, blood collection tubes. We tested impact commonly used (EDTA heparin) specialized tubes storage...
Abstract Langerhans cell histiocytosis (LCH) is a rare neoplasm predominantly affecting children. It occupies hybrid position between cancers and inflammatory diseases, which makes it an attractive model for studying cancer development. To explore the molecular mechanisms underlying pathophysiology of LCH its characteristic clinical heterogeneity, we investigated transcriptomic epigenomic diversity in primary lesions. Using single-cell RNA sequencing, identified multiple recurrent types...
Abstract Langerhans cell histiocytosis (LCH) is a rare neoplastic disorder caused by somatic genetic alterations in hematopoietic precursor cells differentiating into CD1a+/CD207+ histiocytes. LCH clinical manifestation highly heterogeneous. BRAF and MAP2K1 mutations account for ∼80% of driver cells. However, their associations remain incompletely understood. Here, we present an international clinicogenomic study childhood LCH, investigating 377 patients genotyped at least BRAFV600E. MAPK...
Abstract Signaling through the Notch1 receptor is essential for T cell development in thymus. Stromal OP9 cells ectopically expressing Notch ligand Delta-like1 mimic thymic environment by inducing hemopoietic stem to undergo vitro development. also expressed on Pax5−/− pro-B cells, which are clonable lymphoid progenitors with a latent myeloid potential. In this study, we demonstrate that efficiently differentiate into CD4+CD8+ αβ and γδ upon coculture OP9-Delta-like1 cells. of strictly...
The international precision oncology program INFORM enrolls relapsed/refractory pediatric cancer patients for comprehensive molecular analysis. We report a two-year pilot study implementing ex vivo drug sensitivity profiling (DSP) using library of 75-78 clinically relevant drugs. included 132 viable tumor samples from 35 centers in seven countries. DSP was conducted on multicellular fresh tissue spheroid cultures 384-well plates with an overall mean processing time three weeks. In 89 cases...
INFORM is an international pediatric precision oncology registry, prospectively collecting molecular and clinical data of children with recurrent, progressive, or very high-risk malignancies. We have previously identified a subgroup patients improved outcomes on the basis profiling. The present analysis systematically investigates progression-free survival (PFS) overall (OS) receiving matching targeted treatment (MTT) most frequently applied drug classes its correlation underlying...
Single-agent vemurafenib leads to a rapid and sustained clinical response in severe multisystem LCH but does not eradicate the disease.Longitudinal assessment of BRAF V600E during treatment shows that remission can occur despite significant amounts mutated BRAF.
<div>AbstractPurpose:<p>Tropomyosin receptor kinase (TRK) fusions are detected in less than 2% of central nervous system tumors. There limited data on the clinical course affected patients.</p>Experimental Design:<p>We conducted an international retrospective cohort study patients with TRK fusion–driven tumors.</p>Results:<p>A total 119 were identified. The median age at time diagnosis was 4.5 years. majority reported to have a histology consistent...
<p>Supplementary Figure S1. Diagram of population patients.</p>
<p>Supplementary Figure S2. A) Hazard ratio for progression or death according to clinical characteristics. B) LGG: Low-grade glioma, HGG: High-grade glioma</p>
<p>Supplementary Data S1. Definition of response, Description response criteria used by sites to evaluate tumor extend resection, resection sites.</p>