A5040347320- Sarcoma Diagnosis and Treatment
- CAR-T cell therapy research
- Physics and Engineering Research Articles
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Protein Degradation and Inhibitors
- Immune Cell Function and Interaction
- Cancer, Lipids, and Metabolism
- Cancer-related gene regulation
- Virus-based gene therapy research
- History and Theory of Mathematics
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Linguistic research and analysis
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Linguistic Education and Pedagogy
- Bacterial Genetics and Biotechnology
- Cardiac tumors and thrombi
Deutschen Konsortium für Translationale Krebsforschung
2017-2022
Charité - Universitätsmedizin Berlin
2000-2022
German Cancer Society
2022
Children's Cancer Center
2021
German Marine Research Consortium
2020-2021
Cancer Research Center
2021
Technical University of Munich
2011-2020
Klinikum rechts der Isar
2011-2020
München Klinik Schwabing
2017-2020
German Cancer Research Center
2015-2020
Pan-cancer analyses that examine commonalities and differences among various cancer types have emerged as a powerful way to obtain novel insights into biology. Here we present comprehensive analysis of genetic alterations in pan-cancer cohort including 961 tumours from children, adolescents, young adults, comprising 24 distinct molecular cancer. Using standardized workflow, identified marked terms mutation frequency significantly mutated genes comparison previously analysed adult cancers....
Ewing tumors (ET) are highly malignant, localized in bone or soft tissue, and molecularly defined by ews/ets translocations. DNA microarray analysis revealed a relationship of ET to both endothelium fetal neural crest. We identified expression histone methyltransferase enhancer Zeste, Drosophila, Homolog 2 (EZH2) be increased ET. Suppressive activity EZH2 maintains stemness normal malignant cells. Here, we found EWS/FLI1 bound the promoter vivo, induced mesenchymal stem Down-regulation RNA...
Round cell sarcomas harboring CIC‐DUX4 fusions have recently been described as highly aggressive soft tissue tumors of children and young adults. Due to partial morphologic immunohistochemical overlap with Ewing sarcoma (ES), ‐positive generally classified ES‐like managed similarly; however, a systematic comparison at the molecular levels between these two groups has not yet conducted. Based on an initial observation that show nuclear immunoreactivity for WT1 ETS transcription factors, FLI1...
Abstract The inducible costimulator (ICOS) is the newest member of CD28/CD152 receptor family involved in regulating T cell activation. We constructed a soluble-Ig fusion protein extracellular domain human ICOS and used it as probe to characterize expression patterns ligand (ICOSL). ICOSIg did not bind CD80- or CD86-transfected Chinese hamster ovary lines, demonstrating that ICOSL distinct from those ligands identified for CD28/CD152. showed selective binding monocytic B whereas was...
Small blue round cell tumors (SBRCTs) are a heterogenous group of that difficult to diagnose because overlapping morphologic, immunohistochemical, and clinical features. About two-thirds EWSR1-negative SBRCTs associated with CIC-DUX4-related fusions, whereas another small subset shows BCOR-CCNB3 X-chromosomal paracentric inversion. Applying paired-end RNA sequencing an SBRCT index case 44-year-old man, we identified novel BCOR-MAML3 chimeric fusion, which was validated by reverse...
Ewing tumors comprise the second most common type of bone-associated cancer in children and are characterized by oncogenic EWS/FLI1 fusion proteins early metastasis. Compelling evidence suggests that elevated levels intracellular oxidative stress contribute to enhanced aggressiveness numerous cancers, possibly including tumors. Using comprehensive microarray analyses RNA interference, we identified six-transmembrane epithelial antigen prostate 1 (STEAP1)-a membrane-bound mesenchymal stem...
Abstract Interleukin (IL)‐4‐transgenic mice were used as a model system to study the consequences of low levels IL‐4 expression for other cytokines examined by quantitative polymerase chain reaction (PCR). For this purpose, plasmid was constructed which contains, in tandem array, 5′ and 3′ primer sequences specific cytokine genes IL‐1 IL‐6, tumor necrosis factor (TNF), lymphotoxin (LT), interferon (IFN)‐γ β‐actin. During co‐amplification, target control DNA complete primers amount PCR...
Abstract Purpose: Non–small cell lung cancer (NSCLC) is a very common and aggressive malignancy. Survival after resection of tumor especially determined by the occurrence distant metastasis. Matrix metalloproteinases (MMP) support this metastatic process degradation extracellular matrix. Experimental Design: We used DNA microarray technology to examine expression 22 MMPs in 89 surgically treated NSCLC patients. Validation results was done using reverse transcription-PCR immunohistology....
A low mutation rate seems to be a general feature of pediatric cancers, in particular oncofusion gene-driven tumors. Genetically, Ewing sarcoma is defined by balanced chromosomal EWS/ETS translocations, which give rise oncogenic chimeric proteins (EWS-ETS). Other contributing somatic mutations involved disease development have only been observed at frequency.Tumor samples 116 patients were analyzed here. Whole-genome sequencing was performed on two with normal, primary, and relapsed tissue....
Abstract The newly recognized regulators of G protein signaling (RGS) attenuate heterotrimeric pathways. We have cloned an IL-2-induced gene from human T cells, cytokine-responsive 1, which encodes a member the RGS family, RGS16. RGS16 binds Giα and Gqα proteins present in inhibits Gi- Gq-mediated By comparison, mitogen-induced RGS2 Gq but not Gi signaling. Moreover, two genes exhibit marked differences expression patterns. cells is suppressed by elevated cAMP, whereas shows reciprocal...
Exosomes are small RNA- and protein-containing extracellular vesicles (EVs) that thought to mediate hetero- homotypic intercellular communication between normal malignant cells.Tumour-derived exosomes believed promote re-programming of the tumour-associated stroma favour tumour growth metastasis. Currently, have been intensively studied in carcinomas. However, little is known about their existence possible role sarcomas. Here, we report on identification with exosomal features derived from...
Ewing sarcoma, an osteolytic malignancy that mainly affects children and young adults, is characterized by early metastasis to lung bone. In this study, we identified the pro-metastatic gene DKK2 as a highly overexpressed in sarcoma compared with corresponding normal tissues. Using RNA interference, showed was critical for malignant cell outgrowth vitro orthotopic xenograft mouse model vivo. Analysis of invasion potential both settings revealed strong correlation expression invasiveness may...
// Tim Hensel 1,2* , Chiara Giorgi 3,* Oxana Schmidt 1,2 Julia Calzada-Wack 4 Frauke Neff Thorsten Buch 5,6 Felix K. Niggli 3 Beat W. Schäfer Stefan Burdach and Günther H.S. Richter 1 Laboratory for Functional Genomics Transplantation Biology, Children’s Cancer Research Centre Department of Pediatrics, Klinikum rechts der Isar, Technische Universität München, Munich, Germany 2 Comprehensive Center Munich (CCCM), Oncology Center, University Hospital, Zurich,...
Abstract Background Histone acetylation and deacetylation seem processes involved in the pathogenesis of Ewing sarcoma (EwS). Here histone deacetylases (HDAC) class I were investigated. Methods Their role was determined using different inhibitors including TSA, Romidepsin, Entinostat PCI-34051 as well CRISPR/Cas9 HDAC knockouts RNAi. To analyze resulting changes microarray analysis, qRT-PCR, western blotting, Co-IP, proliferation, apoptosis, differentiation, invasion assays xenograft-mouse...
Metastatic spread in Ewing sarcomas (ES) is frequent and haematogenous. G-protein coupled receptor 64 (GPR64), an orphan with normal expression restricted to human epididymis specifically over-expressed ES among sarcoma, but also up-regulated a number of carcinomas derived from prostate, kidney or lung. Inhibition GPR64 by RNA interference impaired colony formation vitro suppressed local tumour growth metastasis Rag2(-/-) γC (-/-) mice. Microarray analysis after knock down revealed...
Pregnancy-associated plasma protein-A (PAPPA), also known as pappalysin, is a member of the insulin-like growth factor (IGF) family. PAPPA acts protease, cleaving IGF inhibitors, i.e., binding proteins (IGFBPs), thereby setting free IGFs. The insulin/IGF-axis involved in cancer general and Ewing sarcoma (ES) particular. ES highly malignant bone tumor characterized by early metastatic spread. associated with various cancers. It overexpressed required for proliferation ES. stimulates normal...
In this study we report the functional comparison of T cell receptor (TCR)-engineered major histocompatibility complex (MHC) class I-restricted CD4+ versus CD8+ cells targeting a peptide from six transmembrane epithelial antigen prostate 1 (STEAP1) in context HLA-A*02:01. STEAP1 is tumor-associated antigen, which overexpressed many cancers, including Ewing sarcoma (EwS). Based on previous observations, postulated strong antitumor potential tumor-redirected transduced with an HLA TCR against...