A5055109962- Immunotherapy and Immune Responses
- Chromosomal and Genetic Variations
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- Viral-associated cancers and disorders
- CAR-T cell therapy research
- Virus-based gene therapy research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- T-cell and Retrovirus Studies
- Sarcoma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Viral Infections and Immunology Research
- Neuroblastoma Research and Treatments
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Multiple Sclerosis Research Studies
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Pancreatic function and diabetes
Martin Luther University Halle-Wittenberg
2015-2024
Wittenberg University
2022
Luther University
2003-2022
University Hospital in Halle
2019
Universitätskinderklinik
2015
Klinik und Poliklinik für Kinder- und Jugendmedizin
2007-2012
Zentrum für Kinderheilkunde
2005
Johannes Gutenberg University Mainz
1993-2003
Heinrich Heine University Düsseldorf
2003
Cancer Research Center
2003
Naturally occurring regulatory T cells (T reg cells) are a thymus-derived subset of cells, which crucial for the maintenance peripheral tolerance by controlling potentially autoreactive cells. However, underlying molecular mechanisms this strictly cell contact–dependent process still elusive. Here we show that naturally harbor high levels cyclic adenosine monophosphate (cAMP). This second messenger is known to be potent inhibitor proliferation and interleukin 2 synthesis in Upon coactivation...
Ewing tumors (ET) are highly malignant, localized in bone or soft tissue, and molecularly defined by ews/ets translocations. DNA microarray analysis revealed a relationship of ET to both endothelium fetal neural crest. We identified expression histone methyltransferase enhancer Zeste, Drosophila, Homolog 2 (EZH2) be increased ET. Suppressive activity EZH2 maintains stemness normal malignant cells. Here, we found EWS/FLI1 bound the promoter vivo, induced mesenchymal stem Down-regulation RNA...
Abstract Ewing family tumors (EFTs) are small round blue cell that show features of neuroectodermal differentiation. However, the histogenetic origin EFTs is still a matter debate. We used high-density DNA microarrays for identification EFT-specific gene expression profiles in comparison with normal tissues diverse origin. identified 37 genes up-regulated compared and validated these by both conventional quantitative reverse transcription-polymerase chain reaction. The pattern EFT-associated...
The product of the proto-oncogene c-myc (myc) is a potent activator cell proliferation. In Burkitt lymphoma (BL), human B-cell tumor, myc consistently found to be transcriptionally activated by chromosomal translocation. mechanisms which promotes cycle progression in B-cells not known. As model for activation BL cells, we have established EBV-EBNA1 positive line, P493-6, expressed under control tetracycline regulated promoter. If expression switched off, P493-6 cells arrest G0/G1 presence...
Chromatin modifications are increasingly recognized as a key mechanism in cancer. The histone methyl-transferase Enhancer of Zeste, Drosophila, Homolog 2 (EZH2), the enzymatic subunit polycomb PRC2 complex methylates H3K27, thereby, mediating gene silencing. EZH2 is over-expressed variety tumor tissue including breast and prostate. Ewing Tumors (ET), alias Peripheral NeuroEctodermal (PNET), highly malignant tumors molecularly defined by ews/ets translocations. We found EWS-FLI1 bound to...
Histone variants play an important role in shaping the mammalian epigenome and their aberrant expression is frequently observed several types of cancer. However, mechanisms that mediate function composition variant-containing chromatin are still largely unknown. A proteomic interrogation containing different H2A macroH2A.1.2, H2A.Bbd revealed a strikingly protein composition. Gene ontology analysis reveals strong enrichment splicing factors as well components replisome H2A.Bbd-containing...
Metastatic spread in Ewing sarcomas (ES) is frequent and haematogenous. G-protein coupled receptor 64 (GPR64), an orphan with normal expression restricted to human epididymis specifically over-expressed ES among sarcoma, but also up-regulated a number of carcinomas derived from prostate, kidney or lung. Inhibition GPR64 by RNA interference impaired colony formation vitro suppressed local tumour growth metastasis Rag2(-/-) γC (-/-) mice. Microarray analysis after knock down revealed...
Lymphoblastoid cell lines, generated by immortalization of normal B cells Epstein–Barr virus (EBV) in vitro , have strong antigen-presenting capacity, are sensitive to EBV-specific cytotoxic T cells, and highly allostimulatory mixed lymphocyte culture. By contrast, EBV-positive Burkitt lymphoma (BL) poor antigen presenters, not recognized poorly allostimulatory, which raises the question whether immunological pressure exerted during BL pathogenesis vivo has selected for a ‘nonimmunogenic’...
The immune pathogenesis of multiple sclerosis (MS) is thought to be triggered by environmental factors in individuals with an unfavorable genetic predisposition. Epstein-Barr virus (EBV) infection a major risk factor for subsequent development MS. Human endogenous retroviruses (HERVs) can activated EBV, and might missing link between initial EBV the later onset In this study, we investigated differential gene expression patterns EBV-immortalized lymphoblastoid B cell lines (LCL) from...
Abstract Background Cancer/testis antigens (CTA) represent a heterogeneous group of expressed nearly exclusively in tumour cells and testis. Recently, we identified phospholipase A1 beta (a CTA also known as lipase member I, LIPI) gene with high expression Ewing family tumours (EFT). In the present paper analyzed LIPI panel normal tissues samples. Procedure The EFT was by using DNA microarray datasets. Expression EFT, other samples, RT‐PCR quantitative RT‐PCR. Results samples but not...
The European Network for Cancer Research in Children and Adolescents (ENCCA) provides an interaction platform stakeholders research care of children with cancer. Among ENCCA objectives is the establishment biology-based prioritization mechanisms selection innovative targets, drugs, prognostic markers validation clinical trials. Specifically sarcomas, there a burning need novel treatment options, since current chemotherapeutic protocols have met their limits. This most obvious metastatic...
Human endogenous retroviruses (ERV) are an integral part of our genome. Expression ERV is usually switched off but reactivation has been observed in varying human diseases including cancer. Recently, associated promoters Hodgkin's lymphoma (HL) cells described. Despite relatively good prognosis, not all patients with HL can be cured the established therapy and this severe late side effects. Therefore, new targets required for development future treatment strategies. Reactivated might...
Abstract Document recommendation systems for locating relevant literature have mostly relied on methods developed a decade ago. This is largely due to the lack of large offline gold-standard benchmark documents that cover variety research fields such newly search techniques can be compared, improved and translated into practice. To overcome this bottleneck, we established RElevant LIterature SearcH consortium consisting more than 1500 scientists from 84 countries, who collectively annotated...
Epstein-Barr virus (EBV) immortalized cells and Burkitt lymphoma have a completely different growth pattern phenotype. EBV express set of 11 viral genes to accommodate B cell activation proliferation, whereas EBV-positive highly the c-myc oncogene that is activated through translocation into 1 immunoglobulin loci EBNA1 as only protein. We developed primary human line conditionally by in which gene program responsible for induction proliferation can be switched on off addition or withdrawal...