A5048008553- Sarcoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Breast Cancer Treatment Studies
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- Cancer and Skin Lesions
- MicroRNA in disease regulation
- Breast Lesions and Carcinomas
- RNA Research and Splicing
- CAR-T cell therapy research
- Genomic variations and chromosomal abnormalities
- HER2/EGFR in Cancer Research
- Lymphoma Diagnosis and Treatment
- Cancer Cells and Metastasis
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Fibroblast Growth Factor Research
- Cell Adhesion Molecules Research
- AI in cancer detection
- Circular RNAs in diseases
- Medical Imaging and Pathology Studies
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
University of Münster
2014-2025
University Hospital Münster
2005-2024
Helmholtz Zentrum München
2010-2014
Technical University of Munich
2014
Klinikum rechts der Isar
2014
National Scientific Center "M.D. Strazhesko Institute of Cardiology"
2010
Nuffield Orthopaedic Centre
2010
University of Oxford
2010
Düsseldorf University Hospital
2010
Olgahospital
2010
Vimentin expression is a rather rare finding in invasive breast cancer, and associated with high tumour invasiveness chemoresistance. It currently explained by two different biological theories: direct histogenetic derivation from myoepithelial cells, epithelial-mesenchymal transition (EMT) reflecting the end-stage of cancer dedifferentiation. In this study we aimed to obtain further insights into hallmarks these vimentin-expressing cancers. We applied immunohistochemistry for vimentin 15...
Early detection of cancers improves patients' survival and decreases the treatment cost. Unfortunately, current methods for diagnosis bladder prostate cancers, two most common urothelial malignancies, suffer from a low sensitivity specificity. MicroRNAs, as group endogenously produced non-coding RNAs, regulate gene expression their is observed to be altered in many cancer progression phenomena. The remarkable stability microRNAs biofluids unique pattern different pathological conditions make...
Abstract Purpose: Osteosarcoma, the most common primary malignant tumor of bone, is characterized by complex karyotypes with numerous structural and numerical alterations. Despite attempts to establish molecular prognostic markers at time diagnosis, accepted predictive factor remains histologic evaluation necrosis after neoadjuvant chemotherapy. The present approach was carried out search for genome-wide recurrent loss heterozygosity copy number variations that could have therapeutic impact...
Hypoxia and the hypoxia-inducible factor (HIF) transcription regulate angiogenic-osteogenic coupling osteoclast-mediated bone resorption. To determine how HIF might coordinate osteoclast osteoblast function, we studied angiopoietin-like 4 (ANGPTL4), top target gene in an Illumina HumanWG-6 v3.0 48k array of normoxic vs. hypoxic osteoclasts differentiated from human CD14(+) monocytes (14.3-fold induction, P<0.0004). ANGPTL4 mRNA protein were induced by 24 h at 2% O(2) primary osteoclasts,...
A low mutation rate seems to be a general feature of pediatric cancers, in particular oncofusion gene-driven tumors. Genetically, Ewing sarcoma is defined by balanced chromosomal EWS/ETS translocations, which give rise oncogenic chimeric proteins (EWS-ETS). Other contributing somatic mutations involved disease development have only been observed at frequency.Tumor samples 116 patients were analyzed here. Whole-genome sequencing was performed on two with normal, primary, and relapsed tissue....
Osteosarcomas (OS) are complex bone tumors with various genomic alterations. These alterations affect the expression and function of several genes due to drastic changes in underlying gene regulatory network. However, we know little about critical regulators their functional consequences on pathogenesis OS. Therefore, aimed determine microRNA transcription factor (TF) co-regulatory networks OS cell proliferation. Cell proliferation is an essential part deeper understanding its regulation...
DNA and RNA nucleotide sequences are an ubiquitous element of all biological cells serving on one hand as a comprehensive library capabilities for the other impressive regulatory system to control cellular function. The presented multi-alignment framework (MAF) should give researchers simple sequence alignment platform functional template, flexible enough adjust steps but also join many different tools custom parameter combinations if in-depth analysis is necessary or advised in e.g. low...
To evaluate the relevance of calcifications for invasive breast cancer detection in population-based digital mammographic screening.This study was approved by an independent ethics committee, and no additional informed consent required. Prospectively documented radiologic features were correlated with pathologic characteristics 241 malignancies diagnosed 24067 participating women aged 50-69 years (part German Screening Program; initial screening rate, 92%; rate [DR], 1.0%; recall [RR],...
Squamous cell carcinoma (SCC) of the oral cavity is a morphological heterogeneous disease. Various cytokeratin (CK) expression patterns with different prognostic values have been described, but little known concerning underlying biological mechanisms. Therefore, present study investigated 193 cases SCCs using immunohistochemistry for α/β/γ‑catenin, glucose transporter 1, caspase‑3, X‑linked inhibitor apoptosis protein, hypoxia inducible factor‑1α, carbonic anhydrase 9, heat shock protein...
Abstract Clear cell sarcoma of soft tissue (CCSST), also known as malignant melanoma parts, represents a rare lesion the musculoskeletal system usually affecting adolescents and young adults. CCSST is typified by chromosomal t(12;22)(q13;q12) translocation resulting in fusion between Ewing gene (EWSR1) activating transcription factor 1 (ATF1), which activity nontransformed cells regulated cyclic AMP. Our aim was to identify critical differentially expressed genes tumor comparison with other...
We identified patterns of differentially-expressed genes in cell lines derived from several pediatric solid tumors. Affymetrix Human Cancer G110 Arrays, carrying 1,700 cancer-associated genes, were applied to a panel 11 originating Ewing tumors (ETs), neuroblastomas, and malignant melanoma soft parts. Hierarchical clustering clearly differentiated these 3 entities revealed groups 75, 102, 36 gene probe-sets exhibiting tumor-type specific up-regulation lines, respectively. Whereas ET...
Gains of chromosomes 7p and 8q are associated with poor prognosis among oestrogen receptor-positive (ER+) stage I/II breast cancer. To identify transcriptional changes this cancer subtype, we applied suppression subtractive hybridisation method to analyse differentially expressed genes six tumours without chromosomal gains. Identified mRNAs were validated by real-time RT-PCR in tissue samples obtained from 186 patients Advanced statistical methods associations mRNA expression distant...
In a prospective observational pilot study on patients undergoing elective cardiac surgery with cardiopulmonary bypass, we evaluated label-free quantitative phase imaging (QPI) digital holographic microscopy (DHM) to describe perioperative inflammation by changes in biophysical cell properties of lymphocytes and monocytes. Blood samples from 25 were investigated prior postoperatively at day 1, 3 6. Biophysical morphological parameters accessible DHM, such as volume, refractive index, dry...
Osteosarcoma (OS) is the most common primary bone cancer exhibiting high genomic instability. This instability affects multiple genes and microRNAs to a varying extent depending on patient tumor subtype. Massive research ongoing identify including their gene products that correlate with disease progression might be used as biomarkers for OS. However, complexity hampers identification of reliable biomarkers. Up now, clinico-pathological factors are key determinants guide prognosis therapeutic...
Osteosarcoma (OS), a bone tumor, exhibit complex karyotype. On the genomic level highly variable degree of alterations in nearly all chromosomal regions and between individual tumors is observable. This hampers identification common drivers OS biology. To identify molecular mechanisms involved maintenance OS, we follow hypothesis that copy number-associated differences patients are intercepted on functional modules. The implementation based network approach utilizing number associated genes...
Hypoxia and the hypoxia-inducible factor (HIF) transcription regulate angiogenic-os-teogenic coupling osteoclast-mediated bone resorption. To determine how HIF might coordinate osteoclast osteoblast function, we studied angiopoi-etin-like 4 (ANGPTL4), top target gene in an Illumina HumanWG-6 v3.0 48k array of normoxic vs. hypoxic osteoclasts differentiated from human CD14+ monocytes (14.3-fold induction, P<0.0004). ANGPTL4 mRNA protein were induced by 24 h at 2% O2 primary osteoclasts,...
A whole chromosome arm loss of 16q belongs to the most frequent and earliest chromosomal alterations in invasive situ breast cancers all common subtypes. Besides E-cadherin, several putative tumour suppressor genes residing on cancer have been investigated. However, significance these findings has remained unclear. Thus, other mechanisms leading gene function (eg haploinsufficiency, or distortion multiple regulative subnetworks) remain be tested as a hypothesis. To define effect expression...
Squamous cell carcinomas (SCCs) are the most prevalent malignant tumours within head and neck. Evidence exists that distinct genes differentially regulated in SCCs of oral cavity compared to other neck regions. Given this background, aim study was investigate whether such tumour site-specific gene expression can also be observed different localizations cavity.Using tissue microarrays (TMAs), we investigated 76 floor mouth, 49 tongue 68 anatomic regions cavity. The 17 involved cycle growth...