Mihaela Zlei
A5078540293- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Multiple Myeloma Research and Treatments
- SARS-CoV-2 and COVID-19 Research
- Acute Lymphoblastic Leukemia research
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Chronic Myeloid Leukemia Treatments
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Listeria monocytogenes in Food Safety
- Immunodeficiency and Autoimmune Disorders
- Oral microbiology and periodontitis research
- Glycosylation and Glycoproteins Research
- Autoimmune Bullous Skin Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Ovarian function and disorders
- Immunotherapy and Immune Responses
- Reproductive Biology and Fertility
- Hematopoietic Stem Cell Transplantation
- Oral Health Pathology and Treatment
Leiden University
2021-2024
Institutul Regional de Oncologie
2015-2024
Leiden University Medical Center
2020-2024
Grigore T. Popa University of Medicine and Pharmacy
2015-2020
Centre Hospitalier Universitaire de Saint-Étienne
2020
Spitalul Clinic Judeţean de Urgenţe "Sf. Spiridon" Iaşi
2004-2007
University Medical Center Freiburg
2006-2007
Virus-specific cellular and humoral responses are major determinants for protection from critical illness after SARS-CoV-2 infection. However, the magnitude of contribution each components to viral clearance remains unclear. Here, we studied timing in relation 122 immune parameters 102 hospitalised patients with moderate severe COVID-19 a longitudinal design. Delayed was associated more disease higher levels SARS-CoV-2-specific (neutralising) antibodies over time, increased numbers...
Abstract The diagnostic spectrum for AML patients is increasingly based on genetic abnormalities due to their prognostic and predictive value. However, information the blast phenotype regarding maturational arrest has started regain importance its power drug responses. Here, we deconvolute 1350 bulk RNA-seq samples from five independent cohorts a single-cell healthy BM reference demonstrate that morphological differentiation stages (FAB) could be faithfully reconstituted using estimated cell...
Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide toward the relevant panel and final diagnosis. In this study, we validated algorithm automated (database-supported) gating identification (AGI tool) cell subsets within samples stained with ALOT. A reference database normal peripheral blood (PB, n = 41) bone marrow (BM; 45) analyzed ALOT was constructed, served as a AGI tool automatically...
Diagnostic services for tuberculosis (TB) are not sufficiently accessible in low-resource settings, where most cases occur, which was aggravated by the COVID-19 pandemic. Early diagnosis of pulmonary TB can reduce transmission. Current TB-diagnostics rely on detection Mycobacterium (Mtb) sputum requiring costly, time-consuming methods, and trained staff. In this study, quantitative lateral flow (LF) assays were used to measure levels seven host proteins sera from pre-COVID-19 patients...
Acute myeloid leukemia (AML) is generally considered a poorly immunogenic malignancy, displaying "non-inflamed" microenvironment, leading to T cell tolerance. However, the immune landscape of AML much more heterogeneous. Since B7 expression regarded as consequence an interferon-mediated "inflammatory" phenotype, we have investigated by flow cytometry checkpoint ligands B7.1, B7.2, PD-L1, PD-L2, ICOS-L, B7-H3, B7-H4 on blasts 30 newly diagnosed patients, and their corresponding receptors...
Abstract SARS-CoV2 infection results in a range of disease severities, but the underlying differential pathogenesis is still not completely understood. At presentation it remains difficult to estimate and predict severity, particular, identify individuals at greatest risk progression towards most severe disease-states. Here we used advanced models with circulating serum analytes as variables combination daily assessment severity using SCODA-score, only single time points also during course...
Abstract Introduction. Several alternative methods to peripheral blood DNA extraction have been implemented so far. Saliva seems represent a very advantageous type of sample, easy harvest and able generate yields comparable those extracted from mononuclear cells. Material methods. 8 patients suspected ankylosing spondylitis, 9 with various hematological malignancies, displaying post-chemotherapy leucopenia 30 healthy volunteers were included in our study. was commercially available kits used...
Wide regional differences in the age-related Anti Mullerian hormone (AMH) regression patterns or age at onset of natural menopause have been reported, possibly reflecting genetic, socioeconomic, environmental, racial ethnic peculiarities. Moreover, adaptation AMH levels from different assays using functions may lack accuracy and externally defined references for not fully comply with a specific geographical area. The current study aimed to establish an accurate mathematical relationship...
Background: Belimumab (BLM), a recombinant human monoclonal antibody directed against B-cell activating factor (BAFF), is the first approved biological agent for patients with active severe systemic lupus erythematosus (SLE) and nephritis (LN). There clinical evidence that combining BLM B cell depleting therapy can ameliorate disease activity in severe, refractory SLE 1 . Although has been shown to significantly decrease total cells, flow cytometry observations suggest rapid increase of...
Objective We aimed to evaluate the prognostic value of circulating tumor cells (CTCs) and impact intraoperative manipulation on CTCs in colorectal cancer (CRC) patients. Methods performed a prospective study 40 patients with CRC stages I IV who received curative surgery using no-touch technique. Flow cytometry was used identify peripheral blood samples (4 mL/sample) collected at two surgical moments: skin incision (T1) after resection (T2). A threshold ≥4 CTCs/4 mL established for...
We report a successful ongoing pregnancy obtained in case of total globozoospermia after intracytoplasmic morphologically selected sperm injection (IMSI) with oocyte activation. The first semen analysis on investigation showed partial globozoospermia. However, under high magnification assessment at retrieval only round headed were observed. Considering the risk complete failure to fertilize from IMSI couple gave written informed consent use activation media post IMSI. One embryo fertilized,...
A primigravid woman with Covid-19 related respiratory insufficiency was admitted into a tertiary Intensive Care Unit at 23 3/7 weeks' gestation. Highly sensitive flow cytometry of peripheral leukocytes indicated significantly suppressed naïve T- and B-cell compartments. The immune cell responses led us keep the initially started administration corticosteroids for fetal maternal indication low dose. After three weeks her response peaked, SARS-CoV-2 cleared clinical improvement ensued week...
Abstract Background: Acute basophilic leukemia is a rare subtype of acute myeloid leukemia, as categorized by the 2008 World Health Organization classification neoplasms. diagnosis requires thorough morphological, cytochemical, immunophenotypic, molecular, and cytogenetic studies exclusion other hematological neoplasms associating basophilia. The disease course defined histamine driven, occasionally life-threatening respiratory, cardiovascular, cutaneous or digestive complications, well...
Abstract Introduction. Metabolic syndrome (MS) is a cluster of distinct metabolic alterations with an increased cardiovascular risk. Peroxisome Proliferator-Activated Receptor - Alpha (PPARα), member the nuclear receptor superfamily transcription factors, critically involved in management lipid metabolism during homeostasis or inflammatory stresses various cell types and represents one therapeutic targets MS. We analysed PPARα expression leukocytes pacients MS, order to address involvement...
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