A5056807229- Multiple Myeloma Research and Treatments
- Chemokine receptors and signaling
- Extracellular vesicles in disease
- Vascular Malformations Diagnosis and Treatment
- Protein Degradation and Inhibitors
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Infectious Diseases and Tuberculosis
- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Head and Neck Surgical Oncology
- Hepatitis B Virus Studies
- Helicobacter pylori-related gastroenterology studies
- Hematological disorders and diagnostics
- Cancer Genomics and Diagnostics
- Blood transfusion and management
- Single-cell and spatial transcriptomics
- Sarcoidosis and Beryllium Toxicity Research
- Systemic Sclerosis and Related Diseases
- Digital Imaging for Blood Diseases
- Renal Transplantation Outcomes and Treatments
- Nutrition and Health in Aging
- Healthcare Systems and Reforms
- Childhood Cancer Survivors' Quality of Life
- IgG4-Related and Inflammatory Diseases
- Viral-associated cancers and disorders
Champalimaud Foundation
2020-2024
Hospital Prof. Dr. Fernando Fonseca
2024
Universidade Nova de Lisboa
1990-2024
Nova Medical (United States)
2022
University of Coimbra
2021
Instituto Português de Oncologia Francisco Gentil
2014-2020
University of Geneva
2013
Instituto Português de Oncologia de Coimbra Francisco Gentil
2008
Flow cytometry has become a highly valuable method to monitor minimal residual disease (MRD) and evaluate the depth of complete response (CR) in bone marrow (BM) multiple myeloma (MM) after therapy. However, current flow-MRD lower sensitivity than molecular methods lacks standardization. Here we report on novel next generation flow (NGF) approach for sensitive standardized MRD detection MM. An optimized 2-tube 8-color antibody panel was constructed five cycles design-evaluation-redesign. In...
Flow cytometric immunophenotyping has become essential for accurate diagnosis, classification, and disease monitoring in hemato-oncology. The EuroFlow Consortium established a fully standardized "all-in-one" pipeline consisting of instrument settings, reagent panels, sample preparation protocols software data analysis classification. For its reproducible implementation, parallel development quality assurance (QA) program was required. Here, we report on the results four consecutive annual...
Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide toward the relevant panel and final diagnosis. In this study, we validated algorithm automated (database-supported) gating identification (AGI tool) cell subsets within samples stained with ALOT. A reference database normal peripheral blood (PB, n = 41) bone marrow (BM; 45) analyzed ALOT was constructed, served as a AGI tool automatically...
Abstract Reproducible expert-independent flow-cytometric criteria for the differential diagnoses between mature B-cell neoplasms are lacking. We developed an algorithm-driven classification these lymphomas by flow cytometry and compared it to WHO gold standard diagnosis. Overall, 662 samples from patients representing 9 disease categories were analyzed at laboratories using previously published EuroFlow 5-tube-8-color chronic lymphoproliferative antibody panel. Expression levels of all 26...
ABSTRACT CD8 T cells play a major role in antiviral immune responses. Their importance for progression to chronic hepatitis C and response treatment are still unclear. To address these issues, virus (HCV)-specific T-cell responses were monitored, at the single-cell level, using HLA class I pentamers specific HCV core NS3 epitopes, 23 chronically infected patients during with pegylated alpha interferon ribavirin. Patients who presented sustained-response therapy had stronger HCV-specific all...
Multiple myeloma (MM), the third most frequent hematological cancer worldwide, is characterized by proliferation of neoplastic plasma cells in bone marrow (BM). One hallmarks MM a permissive BM microenvironment. Increasing evidence suggests that cell-to-cell communication between and immune via tumor cell-derived extracellular vesicles (EV) plays key role pathogenesis MM. Hence, we aimed to explore alterations induced MM-derived EV. For this, inoculated immunocompetent BALB/cByJ mice with...
Abstract The synergy resulting from the high conductivity of graphene and catalytic properties metal nanoparticle has been a resource to improve activity functionality electrochemical sensors. This work focuses on simultaneous synthesis copper nanoparticles (CuNPs) laser‐induced (LIG) derived paper, through one‐step laser processing approach. A chromatography paper substrate with drop‐casted sulfate is used for fabrication this hybrid material, characterized in terms its morphological,...
Multiple myeloma (MM) is a hematological malignancy of clonal antibody-secreting plasma cells (PCs). MM diagnosis and risk stratification rely on bone marrow (BM) biopsy, an invasive procedure prone to sample bias. Liquid biopsies, such as extracellular vesicles (EV) in peripheral blood (PB), hold promise new minimally tools. Real-world studies analyzing patient-derived EV proteome are rare. Here, we characterized small protein content from PB BM samples cohort 102 monoclonal gammopathies...
Objective interpretation of FC results may still be hampered by limited technical standardization. The EuroFlow consortium conducted a series experiments to determine the impact different variables on relative distribution and median fluorescence intensity (MFI) markers stained cell populations, from both healthy donors patients' samples with distinct hematological malignancies. use anticoagulants; time interval between sample collection, preparation, acquisition; pH washing buffers; surface...
The introduction of new and more effective therapeutic options for Multiple Myeloma (MM) has significantly deepened prolonged patients’ remission. As currently used treatment protocols induce high rates complete responses, Measurable Residual Disease (MRD) assessment become essential to enhance the evaluation efficacy. Detection MRD improved with development highly sensitive standardized techniques such as Next Generation Flow or Sequencing, complemented by functional imaging techniques....
<h3>Background:</h3> Nailfold capillaroscopy (PC) is fundamental in the evaluation of RP, helping to differentiate between primary and secondary Raynaud's phenomenon (RP). However, usefulness this technique patients with symptoms suggestive a connective tissue disease (CTD) or positive antibodies, but without has not been established. <h3>Objectives:</h3> To evaluate utility NVC for diagnosis CTD RP. <h3>Methods:</h3> Retrospective review performed RP suspicion CTD, 2010-2022. from an age...
Background Multiple myeloma (MM) is an incurable hematologic malignancy, and even though the complete response (CR) rate has been growing, a high percentage of patients continues to relapse. Recent research showed that most relapses may be related persistence measurable residual disease (MRD). In this study, we intended evaluate MRD status in MM who reached CR their second or third lines treatment. Methods This was cross-sectional, multicentre, non-interventional study describe with relapsed...
Topic: 13. Myeloma and other monoclonal gammopathies - Biology & Translational Research Background: Multiple (MM) is characterised by the accumulation of plasma cells in a dynamic permissive bone marrow (BM) microenvironment. Most immune express neuronal receptors neurotransmitters, allowing crosstalk between these multicellular networks. Interactions neurons have been reported to regulate organ physiology contribute several cancers. NK are important players MM, adrenergic signals were shown...