A5036901558- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Advanced Sensor and Energy Harvesting Materials
- Mesenchymal stem cell research
- Gas Sensing Nanomaterials and Sensors
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- ZnO doping and properties
- Knee injuries and reconstruction techniques
- Transition Metal Oxide Nanomaterials
- Supercapacitor Materials and Fabrication
- Advanced Memory and Neural Computing
- Nanomaterials and Printing Technologies
- Pluripotent Stem Cells Research
- Electrohydrodynamics and Fluid Dynamics
- Analytical Chemistry and Sensors
- Bone Tissue Engineering Materials
- Electrochemical sensors and biosensors
- Fuel Cells and Related Materials
- Quantum Dots Synthesis And Properties
- Polymer composites and self-healing
- Chalcogenide Semiconductor Thin Films
- Flame retardant materials and properties
- 2D Materials and Applications
Universidade Nova de Lisboa
2020-2024
Uninova
2024
Trinity College Dublin
2014-2022
Instituto de Biologia Experimental e Tecnológica
2020-2022
Instituto Politécnico de Leiria
2020
GenIbet (Portugal)
2020
Instituto Português Da Qualidade
2020
University of Coimbra
2017
Direct Laser Writing (DLW) has been increasingly selected as a microfabrication route for efficient, cost-effective, high-resolution material synthesis and conversion. Concurrently, lasers participate in the patterning assembly of functional geometries several fields application, which electronics stand out. In this review, recent advances strategies based on DLW are surveyed outlined, laser growth strategies. First, main parameters influencing transformation mechanisms summarized, aimed at...
The use of engineered cardiac tissue for high-throughput drug screening/toxicology assessment remains largely unexplored. Here we propose a scaffold that mimics aspects extracellular matrix while preserving the contractility cardiomyocytes. is based on poly(caprolactone) (PCL) nanofilm with magnetic properties (MNF, standing nanofilm) coated layer piezoelectric (PIEZO) microfibers poly(vinylidene fluoride–trifluoroethylene) (MNF+PIEZO). creates flexible support cell contraction and aligned...
An alternative strategy to the use of in vitro expanded cells regenerative medicine is freshly isolated stromal cells, where a bioactive scaffold used provide an environment conducive proliferation and tissue-specific differentiation vivo. The objective this study develop cartilage extracellular matrix (ECM)-derived that could facilitate rapid chondrogenic cells. By freeze-drying cryomilled ECM differing concentrations, it possible produce scaffolds with range pore sizes. migration,...
Regenerating articular cartilage and fibrocartilaginous tissue such as the meniscus is still a challenge in orthopedic medicine. While range of different scaffolds have been developed for joint repair, none facilitated development that mimics complexity soft tissues cartilage. Furthermore, many these are not designed to function mechanically challenging environments. The overall goal this study was develop porous, biomimetic, shape-memory alginate scaffold directing regeneration. To end,...
A therapy for regenerating large cartilaginous lesions within the articular surface of osteoarthritic joints remains elusive. While tissue engineering strategies such as matrix-assisted autologous chondrocyte implantation can be used in repair focal cartilage defects, extending approaches to treatment osteoarthritis will require a number scientific and technical challenges overcome. These include identification an abundant source chondroprogenitor cells that maintain their chondrogenic...
Abstract The extracellular matrix (ECM) of engineered human cardiac tissues corresponds to simplistic biomaterials that allow tissue assembly, or animal derived off-the-shelf non-cardiac specific matrices. Decellularized ECM from could provide a means improve the mimicry tissues. Decellularization samples using immersion-based methods can produce acceptable scaffolds; however, these protocols are mostly described for preparations. We have tested four decellularize and evaluated their...
Human induced pluripotent stem cells (hiPSC) possess significant therapeutic potential due to their high self-renewal capability and differentiate into specialized such as cardiomyocytes. However, generated hiPSC-derived cardiomyocytes (hiPSC-CM) are still immature, with phenotypic functional features resembling the fetal rather than adult counterparts, which limits application in cell-based therapies, vitro cardiac disease modeling, drug cardiotoxicity screening. Recent discoveries have...
Successful repair of osteochondral defects is challenging, due in part to their complex gradient nature. Tissue engineering approaches have shown promise with the development layered scaffolds that aim promote cartilage and bone regeneration within defect. The clinical potential implanting these cell-free has been demonstrated, whereby cells from host marrow MSCs infiltrate required regions However, seeding layer scaffold a chondrogenic cell population prior implantation may enhance tissue...
Heart failure with preserved ejection fraction (HFpEF) is a highly prevalent but still poorly understood clinical entity. Its current pathophysiological understanding supports critical role of comorbidities and their chronic effect on cardiac function structure. Importantly, despite the replication some HFpEF phenotypic features, to this day, experimental models have failed bring new effective therapies setting. Thus, direct investigation human myocardial samples may unveil key, possibly...
Numerous growth factor delivery systems have been developed for tissue engineering. However, little is known about how the dose of a specific protein will influence regeneration, or different patients respond to altered levels presentation. The objective present study was assess stem cell chondrogenesis within extracellular-matrix (ECM)-derived scaffolds loaded with escalating transforming (TGF)-β3. It also sought determine if cells display donor-dependent response doses TGF-β3, from low (5...
Osteoarthritis is a leading cause of disability affecting an increasing number individuals. However, cartilage replacement therapies are inadequate, and better regeneration products must be developed. In this work, we describe human mesenchymal stem cell (hMSC)-based approach for fabricating extracellular matrix (ECM) scaffolds from tissue-engineered sheets then inducing chondrogenesis reseeded hMSCs within the ECM scaffolds. Two types were fabricated: one high-density hMSC cultured with...
ECM-derived scaffolds have previously been developed from devitalized native cartilage and successfully used in tissue engineering. Such ECM-based biomaterials are commonly derived animal tissue, which may not represent the ideal source for applications human. Native human ECM can be as an alternative to xenogeneic tissue; however, its supply limited, leading need a more readily available of such biomaterials. The objective this study was compare engineered cartilaginous chondro-permissive...
The combination of cardiomyocytes and non-myocyte cardiac populations, such as endothelial cells mesenchymal cells, has been shown to be critical for recapitulation the human heart tissue in vitro cell-based modeling. However, most current engineered microtissues still relying on either: (i) murine/human limited primary cell sources; (ii) animal-derived undefined hydrogels/matrices with batch-to-batch variability; or (iii) culture systems low compliance pharmacological high throughput...
Abstract The synergy resulting from the high conductivity of graphene and catalytic properties metal nanoparticle has been a resource to improve activity functionality electrochemical sensors. This work focuses on simultaneous synthesis copper nanoparticles (CuNPs) laser‐induced (LIG) derived paper, through one‐step laser processing approach. A chromatography paper substrate with drop‐casted sulfate is used for fabrication this hybrid material, characterized in terms its morphological,...
Nowadays, the standard media used in clinical-scale mesenchymal stem cell (MSC) production to supply hundreds of clinical trials uses animal-derived components as supplements, which raises several health concerns. Consequently, development xeno-free supplements has emerged. In current study, effect pulse electric field (PEF) application platelet concentrates (PC) with no therapeutic value for producing releasates (PR) able sustain ability bone marrow-MSCs (BM-MSCs) self-renew and...
Cartilage has poor regenerative capacity and thus damage to the joint surfaces presents a major clinical challenge. Recent research focussed on development of tissue-engineered cell-based approaches for treatment cartilage osteochondral injuries, with current clinically available including autologous chondrocyte implantation matrix-assisted implantation. However, these have significant disadvantages due requirement two-stage surgical procedure an in vitro expansion phase which increases...